Restriction map for
pFN21AE6381
(Circular) (Six-base) MAPSORT of: pFN21AE6381.seq Check: 9292 from: 1 to: 5662
pFN21AE6381 5662 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2892 3725 5109 5377 1632
Size: 398 862 833 1384 268 1917
Fragments arranged by size:
1917 1384 862 833 398 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 2547 4391 2547
Size: 1844 3818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 2319 3405 4977 2319
Size: 1086 1572 3004
Fragments arranged by size:
3004 1572 1086
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2501 2758 2971 3197 3349 5458 1493
Size: 1008 257 213 226 152 2109 1697
Fragments arranged by size:
2109 1697 1008 257 226 213 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
BamHI G'GATC_C
Cuts at: 2192 2992 3359 2192
Size: 800 367 4495
Fragments arranged by size:
4495 800 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2488 2983 4438 618
Size: 325 217 627 378 323 495 1455 1842
Fragments arranged by size:
1842 1455 627 495 378 325 323 217
BanII G_rGCy'C
Cuts at: 1391 2981 1391
Size: 1590 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3853 3887 1903
Size: 34 50 34 1832 34 3678
Fragments arranged by size:
3678 1832 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2543 3554 4764 2543
Size: 1011 1210 3441
Fragments arranged by size:
3441 1210 1011
BclI T'GATC_A
Cuts at: 1548 2310 2575 1548
Size: 762 265 4635
Fragments arranged by size:
4635 762 265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2512 5657 2512
Size: 3145 2517
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4879 1790
Size: 1933 1156 2573
Fragments arranged by size:
2573 1933 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4322 1239
Size: 3083 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 3787 4652 4950 2558
Size: 1229 865 298 3270
Fragments arranged by size:
3270 1229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 1186
Size: 1705 3957
BseYI C'CCAG_C
Cuts at: 2319 2801 4865 5361 2319
Size: 482 2064 496 2620
Fragments arranged by size:
2620 2064 496 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2241 1163
Size: 573 505 4584
Fragments arranged by size:
4584 573 505
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3723 3808 4879 3723
Size: 85 1071 4506
Fragments arranged by size:
4506 1071 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
BsmBI CGTCTCn'nnnn_
Cuts at: 2534 2534
Size: 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2981 3723 3808 4879 1391
Size: 399 1191 742 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2213 2649 3017 844
Size: 1369 436 368 3489
Fragments arranged by size:
3489 1369 436 368
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 2714 1184
Size: 1530 4132
BstYI r'GATC_y
Cuts at: 1972 2192 2512 2992 3359 3744 3761 5213 5299
Size: 220 320 480 367 385 17 1452 86
Cuts at: 5299 5311 5657 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 480 385 367 346 320 220
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2934 3109 3998
Size: 54 1425 50 275 18 1104 175 889
Cuts at: 3998 5627 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1104 889 275 175 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2148 2214 2911 3592 1353
Size: 653 142 66 697 681 3423
Fragments arranged by size:
3423 697 681 653 142 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2892 3725 4322 5109 5377 1239
Size: 393 398 862 833 597 787 268 1524
Fragments arranged by size:
1524 862 833 787 597 398 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2832 2832
Size: 5662
EcoO109I rG'GnC_Cy
Cuts at: 2225 2225
Size: 5662
EcoRI G'AATT_C
Cuts at: 2758 2971 3349 2758
Size: 213 378 5071
Fragments arranged by size:
5071 378 213
EcoRV GAT'ATC
Cuts at: 2306 2306
Size: 5662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2352 2352
Size: 5662
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 2533 2565 4303
Size: 32 135 32 723 32 206 32 1738
Cuts at: 4303 4335 4377 4409 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1738 723 206 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2053 2165 4776 4960 1305
Size: 748 112 2611 184 2007
Fragments arranged by size:
2611 2007 748 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3840
Fragments arranged by size:
3840 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3620 3979 4138 518
Size: 648 2454 359 159 2042
Fragments arranged by size:
2454 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2319 3078 3755 4903 5148 1606
Size: 713 759 677 1148 245 2120
Fragments arranged by size:
2120 1148 759 713 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5110
NdeI CA'TA_TG
Cuts at: 387 2891 387
Size: 2504 3158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2225 2225
Size: 5662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2319 2319
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2445 2763 2829 3010 4145 4826 5017
Size: 219 1400 318 66 181 1135 681 191
Cuts at: 5017 826
Size: 1471
Fragments arranged by size:
1471 1400 1135 681 318 219 191 181
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2537 3766 4667 4929 820
Size: 197 926 594 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 594 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 3101 513
Size: 552 2036 3074
Fragments arranged by size:
3074 2036 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3689 3706 3859 4044 4489 325
Size: 1255 2109 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2737 3908 5620
Size: 274 80 33 51 1443 760 1171 1712
Cuts at: 5620 96
Size: 138
Fragments arranged by size:
1712 1443 1171 760 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2317 2349 2650 2682 5622 5654 2317
Size: 32 301 32 2940 32 2325
Fragments arranged by size:
2940 2325 301 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 2864
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI