Restriction map for
pFN21AE6380
(Circular) (Six-base) MAPSORT of: pFN21AE6380.seq Check: 4114 from: 1 to: 5656
pFN21AE6380 5656 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2035 2977 2035
Size: 942 4714
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2061 2640 3719 5103 5371 1632
Size: 429 579 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1079 579 429 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 2395 4385 2395
Size: 1990 3666
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2138 2655 1184
Size: 954 517 4185
Fragments arranged by size:
4185 954 517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 2040
Size: 32 5624
AlwNI CAG_nnn'CTG
Cuts at: 2408 3399 4971 2408
Size: 991 1572 3093
Fragments arranged by size:
3093 1572 991
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2359 2965 3191 3343 5452 1493
Size: 866 606 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2230 2981 1870
Size: 73 235 52 751 4545
Fragments arranged by size:
4545 751 235 73 52
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2977 4432 618
Size: 325 217 627 248 942 1455 1842
Fragments arranged by size:
1842 1455 942 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2058 2385 2975 1391
Size: 667 327 590 4072
Fragments arranged by size:
4072 667 590 327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2529 2659 5651 2529
Size: 130 2992 2534
Fragments arranged by size:
2992 2534 130
BlpI GC'TnA_GC
Cuts at: 2910 3073 2910
Size: 163 5493
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2065 2879 3717 4873 1790
Size: 234 41 814 838 1156 2573
Fragments arranged by size:
2573 1156 838 814 234 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2576 2588 2669 4316 1239
Size: 851 486 12 81 1647 2579
Fragments arranged by size:
2579 1647 851 486 81 12
Bpu10I CC'TnA_GC
Cuts at: 2379 2379
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2813 3781 4646 4944 2813
Size: 968 865 298 3525
Fragments arranged by size:
3525 968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 4319 882
Size: 617 669 2151 2219
Fragments arranged by size:
2219 2151 669 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2649 4089 4487 4761 4908 5319 2649
Size: 1440 398 274 147 411 2986
Fragments arranged by size:
2986 1440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2040 2070 1178
Size: 30 165 30 637 30 4764
Fragments arranged by size:
4764 637 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2198 2252 1186
Size: 1000 12 54 4590
Fragments arranged by size:
4590 1000 54 12
BseYI C'CCAG_C
Cuts at: 2698 4859 5355 2698
Size: 2161 496 2999
Fragments arranged by size:
2999 2161 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 2234 2401 1163
Size: 573 398 100 167 4418
Fragments arranged by size:
4418 573 398 167 100
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2385 3717 3802 4873 2058
Size: 327 1332 85 1071 2841
Fragments arranged by size:
2841 1332 1071 327 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2058 2065 2385 2879 2975 3717
Size: 399 234 34 7 320 494 96 742
Cuts at: 3717 3802 4873 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 320 234 96
85 34 7
BspEI T'CCGG_A
Cuts at: 2649 5319 2649
Size: 2670 2986
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2529 2659 2986 3353 3738 3755 5207 5293
Size: 557 130 327 367 385 17 1452 86
Cuts at: 5293 5305 5651 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 557 385 367 346 327 130
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2092 2253 2735 2809 513
Size: 552 1027 161 482 74 3360
Fragments arranged by size:
3360 1027 552 482 161 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2196 3172 3966 3986 2196
Size: 976 794 20 3866
Fragments arranged by size:
3866 976 794 20
ClaI AT'CG_AT
Cuts at: 1554 2844 1554
Size: 1290 4366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2467 3103
Size: 54 1425 50 275 18 420 217 636
Cuts at: 3103 3992 5621 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 636 420 275 217 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2489 2774 3586 1353
Size: 684 452 285 812 3423
Fragments arranged by size:
3423 812 684 452 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2273 4243 4617 4763 387
Size: 1064 138 684 1970 374 146 1280
Fragments arranged by size:
1970 1280 1064 684 374 146 138
EcoICRI GAG'CTC
Cuts at: 2056 2383 2056
Size: 327 5329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2090 2576 2588 2640 2669 3719
Size: 393 429 29 486 12 52 29 1050
Cuts at: 3719 4316 5103 5371 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1050 787 597 486 429 393 268
52 29 29 12
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 5656
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2644 2676 4297
Size: 32 135 32 809 32 231 32 1621
Cuts at: 4297 4329 4371 4403 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1621 809 231 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2039 2981 2039
Size: 942 4714
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2814 3614 3973 4132 518
Size: 648 1648 800 359 159 2042
Fragments arranged by size:
2042 1648 800 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2283 2415 3072 3749 4897 5142 1606
Size: 516 161 132 657 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 516 245 161 132
NcoI C'CATG_G
Cuts at: 513 1065 2092 2735 513
Size: 552 1027 643 3434
Fragments arranged by size:
3434 1027 643 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2683 3014 4559 1132
Size: 97 1454 331 1545 2229
Fragments arranged by size:
2229 1545 1454 331 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PfoI T'CCnGG_A
Cuts at: 2553 2685 2553
Size: 132 5524
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2040 2072 3725 3757 5238 5270 2040
Size: 32 1653 32 1481 32 2426
Fragments arranged by size:
2426 1653 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2205 2373 3008 830
Size: 1375 168 635 3478
Fragments arranged by size:
3478 1375 635 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2122 2122
Size: 5656
SacI G_AGCT'C
Cuts at: 2058 2385 2058
Size: 327 5329
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SapI GCTCTTCn'nnn_
Cuts at: 2489 2774 2489
Size: 285 5371
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2201 2369 3004 4139 4820 5011 826
Size: 219 1156 168 635 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 681 635 219 191 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2180 2983 1872
Size: 308 803 4545
Fragments arranged by size:
4545 803 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2792 3760 4661 4923 820
Size: 197 926 849 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 849 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2085 2092 2148 2735 3095 513
Size: 552 1020 7 56 587 360 3074
Fragments arranged by size:
3074 1020 587 552 360 56 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2178 2981 1870
Size: 308 803 4545
Fragments arranged by size:
4545 803 308
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI