Restriction map for
pFN21AE6370
(Circular) (Six-base) MAPSORT of: pFN21AE6370.seq Check: 4544 from: 1 to: 5683
pFN21AE6370 5683 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2658 2658
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2492 3746 5130 5398 1632
Size: 860 1254 1384 268 1917
Fragments arranged by size:
1917 1384 1254 860 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2340 2664 4582 5559 1203
Size: 1137 324 1918 977 1327
Fragments arranged by size:
1918 1327 1137 977 324
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2792 1184
Size: 1608 4075
AlwNI CAG_nnn'CTG
Cuts at: 2912 3426 4998 2912
Size: 514 1572 3597
Fragments arranged by size:
3597 1572 514
ApaI G_GGCC'C
Cuts at: 2033 2043 2747 2937 2033
Size: 10 704 190 4779
Fragments arranged by size:
4779 704 190 10
ApaLI G'TGCA_C
Cuts at: 2234 3740 4896 2234
Size: 1506 1156 3021
Fragments arranged by size:
3021 1506 1156
ApoI r'AATT_y
Cuts at: 1493 2759 2992 3218 3370 5479 1493
Size: 1266 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1266 233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2088 2204 3008 1870
Size: 73 93 52 116 804 4545
Fragments arranged by size:
4545 804 116 93 73 52
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2643 3004 4459 618
Size: 325 217 627 856 361 1455 1842
Fragments arranged by size:
1842 1455 856 627 361 325 217
BanII G_rGCy'C
Cuts at: 1391 2033 2043 2640 2747 2937 3002 1391
Size: 642 10 597 107 190 65 4072
Fragments arranged by size:
4072 642 597 190 107 65 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2480 928
Size: 971 581 4131
Fragments arranged by size:
4131 971 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2963 4294 136
Size: 107 122 71 631 605 1291 1331 1525
Fragments arranged by size:
1525 1331 1291 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 2901 3100 2901
Size: 199 5484
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2043 2238 2747 2937 3744 4900 1790
Size: 243 10 195 509 190 807 1156 2573
Fragments arranged by size:
2573 1156 807 509 243 195 190 10
BmgBI CAC'GTC
Cuts at: 1318 2254 2455 1318
Size: 936 201 4546
Fragments arranged by size:
4546 936 201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 2380 3414
Size: 416 53 83 399 24 876 87 1034
Cuts at: 3414 4372 442
Size: 958 1753
Fragments arranged by size:
1753 1034 958 876 416 399 87 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2508 2654 2850 2907 4343 1239
Size: 1269 146 196 57 1436 2579
Fragments arranged by size:
2579 1436 1269 196 146 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 4346 882
Size: 617 1086 1761 2219
Fragments arranged by size:
2219 1761 1086 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 3474 493
Size: 711 1143 1127 2702
Fragments arranged by size:
2702 1143 1127 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 3490 3872 4507
Size: 53 83 186 936 660 1297 382 635
Cuts at: 4507 275
Size: 1451
Fragments arranged by size:
1451 1297 936 660 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2439 2469 2627 2657 2878
Size: 30 165 30 1036 30 158 30 221
Cuts at: 2878 2908 1178
Size: 30 3953
Fragments arranged by size:
3953 1036 221 165 158 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5683
BseYI C'CCAG_C
Cuts at: 2165 2553 2696 2796 4886 5382 2165
Size: 388 143 100 2090 496 2466
Fragments arranged by size:
2466 2090 496 388 143 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 2486 2636 1163
Size: 573 663 87 150 4210
Fragments arranged by size:
4210 663 573 150 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2505 3744 3829 4900 2238
Size: 267 1239 85 1071 3021
Fragments arranged by size:
3021 1239 1071 267 85
BsmI GAATG_Cn'
Cuts at: 2716 3194 3287 2716
Size: 478 93 5112
Fragments arranged by size:
5112 478 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2043 2238 2505 2640 2747 2937
Size: 399 243 10 195 267 135 107 190
Cuts at: 2937 3002 3744 3829 4900 1391
Size: 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 243 195 190
135 107 85 65 10
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2658 3038 844
Size: 1371 443 380 3489
Fragments arranged by size:
3489 1371 443 380
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 794
Size: 1820 3863
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678
Size: 1041 367 385 17 1452 86 12 346
Cuts at: 5678 1972
Size: 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2363 2363
Size: 5683
BtgI C'CryG_G
Cuts at: 513 1065 2004 2219 513
Size: 552 939 215 3977
Fragments arranged by size:
3977 939 552 215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2302 3199 3993 4013 2302
Size: 897 794 20 3972
Fragments arranged by size:
3972 897 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DraIII CAC_nnn'GTG
Cuts at: 2388 2388
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2216 2955 3130
Size: 54 1425 50 275 18 386 739 175
Cuts at: 3130 4019 5648 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 739 386 275 175 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3613 1353
Size: 2260 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2508 2654 2850 2907 3746 4343
Size: 393 860 16 146 196 57 839 597
Cuts at: 4343 5130 5398 1239
Size: 787 268 1524
Fragments arranged by size:
1524 860 839 787 597 393 268 196
146 57 16
EcoO109I rG'GnC_Cy
Cuts at: 2040 2743 2933 2040
Size: 703 190 4790
Fragments arranged by size:
4790 703 190
EcoRI G'AATT_C
Cuts at: 2759 2992 3370 2759
Size: 233 378 5072
Fragments arranged by size:
5072 378 233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2552 2552
Size: 5683
FspI TGC'GCA
Cuts at: 2301 4189 2301
Size: 1888 3795
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373
Size: 32 135 32 2752 32 42 32 2626
Fragments arranged by size:
2752 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 2548 3282 2548
Size: 734 4949
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2838 4797 4981 1305
Size: 1533 1959 184 2007
Fragments arranged by size:
2007 1959 1533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2957 10
Size: 54 1425 343 1125 2736
Fragments arranged by size:
2736 1425 1125 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2297 2470 2626 2772 3099 3776 4924 5169
Size: 691 173 156 146 327 677 1148 245
Cuts at: 5169 1606
Size: 2120
Fragments arranged by size:
2120 1148 691 677 327 245 173 156
146
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 2425 387
Size: 2038 3645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2344 2668 2689 3041 4586 1132
Size: 97 1115 324 21 352 1545 2229
Fragments arranged by size:
2229 1545 1115 352 324 97 21
PasI CC'CwG_GG
Cuts at: 1348 2050 1348
Size: 702 4981
PciI A'CATG_T
Cuts at: 2340 2664 4582 2340
Size: 324 1918 3441
Fragments arranged by size:
3441 1918 324
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2553 1108
Size: 1445 4238
PfoI T'CCnGG_A
Cuts at: 2631 2631
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2665 1613
Size: 1052 4631
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2029 2039 2743 2933 2029
Size: 10 704 190 4779
Fragments arranged by size:
4779 704 190 10
PstI C_TGCA'G
Cuts at: 830 2610 3035 830
Size: 1780 425 3478
Fragments arranged by size:
3478 1780 425
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2297 2470 2772 2297
Size: 173 302 5208
Fragments arranged by size:
5208 302 173
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 2282 3931 5643 2282
Size: 1649 1712 2322
Fragments arranged by size:
2322 1712 1649
SfcI C'TryA_G
Cuts at: 826 1045 2606 3031 4166 4847 5038 826
Size: 219 1561 425 1135 681 191 1471
Fragments arranged by size:
1561 1471 1135 681 425 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2090 3010 1872
Size: 218 920 4545
Fragments arranged by size:
4545 920 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2347 3474 493
Size: 1854 1127 2702
Fragments arranged by size:
2702 1854 1127
SpeI A'CTAG_T
Cuts at: 152 2572 152
Size: 2420 3263
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2247 2352 1517
Size: 730 105 4848
Fragments arranged by size:
4848 730 105
StyI C'CwwG_G
Cuts at: 513 1065 2004 3122 513
Size: 552 939 1118 3074
Fragments arranged by size:
3074 1118 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2275 2399 3710 3727 3880 4065 4510
Size: 1255 695 124 1311 17 153 185 445
Cuts at: 4510 325
Size: 1498
Fragments arranged by size:
1498 1311 1255 695 445 185 153 124
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 3929 5641
Size: 274 80 33 51 1443 303 1649 1712
Cuts at: 5641 96
Size: 138
Fragments arranged by size:
1712 1649 1443 303 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 2627 2659 2878 2910 5643 5675 2439
Size: 32 156 32 219 32 2733 32 2447
Fragments arranged by size:
2733 2447 219 156 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2088 3008 1870
Size: 218 920 4545
Fragments arranged by size:
4545 920 218
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI