Restriction map for
pFN21AE6368
(Circular) (Six-base) MAPSORT of: pFN21AE6368.seq Check: 1914 from: 1 to: 5788
pFN21AE6368 5788 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2055 2055
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 2352 3109 2352
Size: 757 5031
AclI AA'CG_TT
Cuts at: 1563 2771 3915 4288 1563
Size: 1208 1144 373 3063
Fragments arranged by size:
3063 1208 1144 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3003 3851 5235 5503 1632
Size: 650 721 848 1384 268 1917
Fragments arranged by size:
1917 1384 848 721 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 2355 4619 2355
Size: 2264 3524
AhdI GACnn_n'nnGTC
Cuts at: 2408 4517 2408
Size: 2109 3679
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2149 2286 3016 3097 3323 3475 5584 1493
Size: 656 137 730 81 226 152 2109 1697
Fragments arranged by size:
2109 1697 730 656 226 152 137 81
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2239 3113 1870
Size: 73 296 874 4545
Fragments arranged by size:
4545 874 296 73
BamHI G'GATC_C
Cuts at: 2243 3118 3485 2243
Size: 875 367 4546
Fragments arranged by size:
4546 875 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2180 2352 2705 3109
Size: 325 217 627 249 144 172 353 404
Cuts at: 3109 4564 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 404 353 325 249 217
172 144
BanII G_rGCy'C
Cuts at: 1391 2761 3107 1391
Size: 1370 346 4072
Fragments arranged by size:
4072 1370 346
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 4693
Fragments arranged by size:
4693 971 124
BbvCI CC'TCA_GC
Cuts at: 2104 2104
Size: 5788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2039 3849 5005 1790
Size: 249 1810 1156 2573
Fragments arranged by size:
2573 1810 1156 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 3519 4477
Size: 416 53 83 399 24 900 1202 958
Cuts at: 4477 442
Size: 1753
Fragments arranged by size:
1753 1202 958 900 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 2715 4448 1239
Size: 1328 148 1733 2579
Fragments arranged by size:
2579 1733 1328 148
Bpu10I CC'TnA_GC
Cuts at: 2104 2104
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2353 2616 2840 3913 4778 5076 2353
Size: 263 224 1073 865 298 3065
Fragments arranged by size:
3065 1073 865 298 263 224
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2631 2655 4451 882
Size: 617 1132 24 1796 2219
Fragments arranged by size:
2219 1796 1132 617 24
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2355 4221 4619 4893 5040 5451 2355
Size: 1866 398 274 147 411 2692
Fragments arranged by size:
2692 1866 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 2864 2894 1178
Size: 30 165 30 1011 30 420 30 4072
Fragments arranged by size:
4072 1011 420 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2619 1186
Size: 946 487 4355
Fragments arranged by size:
4355 946 487
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2761 3849 3934 5005 2761
Size: 1088 85 1071 3544
Fragments arranged by size:
3544 1088 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
BsmBI CGTCTCn'nnnn_
Cuts at: 2976 2976
Size: 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2761 3107 3849 3934 5005 1391
Size: 399 249 722 346 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 399 346 249 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 2839 3677 5407 2839
Size: 838 1730 3220
Fragments arranged by size:
3220 1730 838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 3143 844
Size: 1211 1088 3489
Fragments arranged by size:
3489 1211 1088
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2219 2505 4283 4457 65
Size: 1183 971 286 1778 174 1396
Fragments arranged by size:
1778 1396 1183 971 286 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2022 2355 4432 4619 1700
Size: 110 212 333 2077 187 2869
Fragments arranged by size:
2869 2077 333 212 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 2517 3848 4860 2517
Size: 1331 1012 3445
Fragments arranged by size:
3445 1331 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2278 794
Size: 1484 4304
BstBI TT'CG_AA
Cuts at: 2808 4592 2808
Size: 1784 4004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2720 2720
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2243 3118 3485 3870 3887 5339 5425 5437
Size: 271 875 367 385 17 1452 86 12
Cuts at: 5437 5783 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 875 385 367 346 271 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3304
Fragments arranged by size:
3304 993 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 3304 4098 4118 2391
Size: 913 794 20 4061
Fragments arranged by size:
4061 913 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2111 3235
Size: 54 1425 50 275 18 231 50 1124
Cuts at: 3235 4124 5753 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1124 889 275 231 54 50
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 2601 2935 3718 1353
Size: 813 435 334 783 3423
Fragments arranged by size:
3423 813 783 435 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2028 4375 4749 4895 387
Size: 1064 138 439 2347 374 146 1280
Fragments arranged by size:
2347 1280 1064 439 374 146 138
EcoICRI GAG'CTC
Cuts at: 2759 2759
Size: 5788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2567 2715 3003 3851 4448 5235
Size: 393 650 285 148 288 848 597 787
Cuts at: 5235 5503 1239
Size: 268 1524
Fragments arranged by size:
1524 848 787 650 597 393 288 285
268 148
EcoRI G'AATT_C
Cuts at: 2149 2286 3097 3475 2149
Size: 137 811 378 4462
Fragments arranged by size:
4462 811 378 137
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2394 2426 2743 2775 2956
Size: 32 135 32 822 32 317 32 181
Cuts at: 2956 2988 4429 4461 4503 4535 1373
Size: 32 1441 32 42 32 2626
Fragments arranged by size:
2626 1441 822 317 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 2356 3113 2356
Size: 757 5031
MfeI C'AATT_G
Cuts at: 2823 3387 2823
Size: 564 5224
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 3051 4902 5086 1305
Size: 1182 564 1851 184 2007
Fragments arranged by size:
2007 1851 1182 564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2113 10
Size: 54 1425 343 281 3685
Fragments arranged by size:
3685 1425 343 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3746 4105 4264 518
Size: 648 2580 359 159 2042
Fragments arranged by size:
2580 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2587 3204 3881 5029 5274 1606
Size: 981 617 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 677 617 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3304
Fragments arranged by size:
3304 993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2073 1348
Size: 725 5063
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2587 2587
Size: 5788
SacI G_AGCT'C
Cuts at: 2761 2761
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SapI GCTCTTCn'nnn_
Cuts at: 2166 2166
Size: 5788
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 3136 4271 4952 5143 826
Size: 219 2091 1135 681 191 1471
Fragments arranged by size:
2091 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 2022 1700
Size: 322 5466
SmaI CCC'GGG
Cuts at: 1872 2241 3115 1872
Size: 369 874 4545
Fragments arranged by size:
4545 874 369
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2332 2631 2855 3892 4793 5055
Size: 197 926 389 299 224 1037 901 262
Cuts at: 5055 820
Size: 1553
Fragments arranged by size:
1553 1037 926 901 389 299 262 224
197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2004 2997 3227 513
Size: 552 939 993 230 3074
Fragments arranged by size:
3074 993 939 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2703 3815 3832 3985 4170 4615 325
Size: 1255 1123 1112 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1123 1112 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2371 2403 2414 2446 5748 5780 2371
Size: 32 11 32 3302 32 2379
Fragments arranged by size:
3302 2379 32 32 32 11
Tth111I GACn'n_nGTC
Cuts at: 1316 2309 1316
Size: 993 4795
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2239 3113 1870
Size: 369 874 4545
Fragments arranged by size:
4545 874 369
XmnI GAAnn'nnTTC
Cuts at: 2814 3078 3917 2814
Size: 264 839 4685
Fragments arranged by size:
4685 839 264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI