Restriction map for
pFN21AE6361
(Circular) (Six-base) MAPSORT of: pFN21AE6361.seq Check: 3606 from: 1 to: 5749
pFN21AE6361 5749 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2756 2756
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 5196 5464 1632
Size: 2180 1384 268 1917
Fragments arranged by size:
2180 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2210 820
Size: 197 1193 4359
Fragments arranged by size:
4359 1193 197
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 3492 5064 3492
Size: 1572 4177
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2819 4303 160
Size: 872 1787 1484 1606
Fragments arranged by size:
1787 1606 1484 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2703 3074 1870
Size: 73 760 371 4545
Fragments arranged by size:
4545 760 371 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 3070 4525 618
Size: 325 217 627 459 824 1455 1842
Fragments arranged by size:
1842 1455 824 627 459 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 2051 3068 1391
Size: 628 32 1017 4072
Fragments arranged by size:
4072 1017 628 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BbvCI CC'TCA_GC
Cuts at: 2136 2136
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2213 2247 3940 3974 1903
Size: 34 50 34 192 34 1693 34 3678
Fragments arranged by size:
3678 1693 192 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2249 3810 4966 1790
Size: 459 1561 1156 2573
Fragments arranged by size:
2573 1561 1156 459
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2388 1056
Size: 1332 4417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4409 1239
Size: 3170 2579
Bpu10I CC'TnA_GC
Cuts at: 2021 2136 2021
Size: 115 5634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2955 3874 4739 5037 2955
Size: 919 865 298 3667
Fragments arranged by size:
3667 919 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2235 4412 882
Size: 617 736 2177 2219
Fragments arranged by size:
2219 2177 736 617
BsaAI yAC'GTr
Cuts at: 493 1204 2999 3540 493
Size: 711 1795 541 2702
Fragments arranged by size:
2702 1795 711 541
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2740 1186
Size: 1554 4195
BseYI C'CCAG_C
Cuts at: 2011 2127 2634 4952 5448 2011
Size: 116 507 2318 496 2312
Fragments arranged by size:
2318 2312 507 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 2784 1163
Size: 573 620 428 4128
Fragments arranged by size:
4128 620 573 428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2051 3810 3895 4966 2051
Size: 1759 85 1071 2834
Fragments arranged by size:
2834 1759 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2051 2249 3068 3810 3895 4966
Size: 399 229 32 198 819 742 85 1071
Cuts at: 4966 1391
Size: 2174
Fragments arranged by size:
2174 1071 819 742 399 229 198 85
32
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 3104 844
Size: 1912 348 3489
Fragments arranged by size:
3489 1912 348
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2113 4393 4580 1700
Size: 110 303 2280 187 2869
Fragments arranged by size:
2869 2280 303 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 3006 3079 3446 3831 3848 5300 5386 5398
Size: 1034 73 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1034 385 367 346 86 73
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2273 513
Size: 552 1208 3989
Fragments arranged by size:
3989 1208 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2368 2550 3265 4059 4079 2368
Size: 182 715 794 20 4038
Fragments arranged by size:
4038 794 715 182 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DraIII CAC_nnn'GTG
Cuts at: 2619 2619
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2270 2302 3001
Size: 54 1425 50 275 18 440 32 699
Cuts at: 3001 3196 4085 5714 8
Size: 195 889 1629 43
Fragments arranged by size:
1629 1425 889 699 440 275 195 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2887 3679 1353
Size: 1534 792 3423
Fragments arranged by size:
3423 1534 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2179 4336 4710 4856 387
Size: 1064 138 590 2157 374 146 1280
Fragments arranged by size:
2157 1280 1064 590 374 146 138
EcoICRI GAG'CTC
Cuts at: 2049 2049
Size: 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3812 4409 5196 5464 1239
Size: 393 2180 597 787 268 1524
Fragments arranged by size:
2180 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2083 2083
Size: 5749
EcoO109I rG'GnC_Cy
Cuts at: 2353 2353
Size: 5749
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2996 2996
Size: 5749
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2033 4896 1678
Size: 355 2863 2531
Fragments arranged by size:
2863 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 4390 4422 4464
Size: 32 135 32 485 32 2301 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 2301 485 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3003 10
Size: 54 1425 343 1171 2756
Fragments arranged by size:
2756 1425 1171 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2272 2322 2821 2883 3707 4066 4225
Size: 648 1106 50 499 62 824 359 159
Cuts at: 4225 518
Size: 2042
Fragments arranged by size:
2042 1106 824 648 499 359 159 62
50
MspA1I CmG'CkG
Cuts at: 1606 2363 2408 3165 3842 4990 5235 1606
Size: 757 45 757 677 1148 245 2120
Fragments arranged by size:
2120 1148 757 757 677 245 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 2384 1052
Size: 1332 4417
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2186 3107 4652 1132
Size: 97 957 921 1545 2229
Fragments arranged by size:
2229 1545 957 921 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 1108
Size: 1494 4255
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 2353
Size: 5749
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 2627 3319 2627
Size: 692 5057
PstI C_TGCA'G
Cuts at: 830 2292 3101 830
Size: 1462 809 3478
Fragments arranged by size:
3478 1462 809
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2363 2408 2363
Size: 45 5704
SacI G_AGCT'C
Cuts at: 2051 2051
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2288 3097 4232 4913 5104 826
Size: 219 1243 809 1135 681 191 1471
Fragments arranged by size:
1471 1243 1135 809 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 2703 2970 3853 4754 5016
Size: 197 926 267 493 267 883 901 262
Cuts at: 5016 820
Size: 1553
Fragments arranged by size:
1553 926 901 883 493 267 267 262
197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 2186 3107 1132
Size: 1054 921 3774
Fragments arranged by size:
3774 1054 921
SspI AAT'ATT
Cuts at: 5 52 2671 2992 3673 5
Size: 47 2619 321 681 2081
Fragments arranged by size:
2619 2081 681 321 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 3188 513
Size: 552 2123 3074
Fragments arranged by size:
3074 2123 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 3776 3793 3946 4131 4576 325
Size: 1255 686 1510 17 153 185 445 1498
Fragments arranged by size:
1510 1498 1255 686 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 5709 5741 2322
Size: 32 3355 32 2330
Fragments arranged by size:
3355 2330 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 2703 1943
Size: 760 4989
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI