Restriction map for
pFN21AE6358
(Circular) (Six-base) MAPSORT of: pFN21AE6358.seq Check: 4729 from: 1 to: 5683
pFN21AE6358 5683 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2421 4582 5559 1203
Size: 1218 2161 977 1327
Fragments arranged by size:
2161 1327 1218 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 2191 4412 2191
Size: 2221 3462
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2560 3426 4998 2560
Size: 866 1572 3245
Fragments arranged by size:
3245 1572 866
ApaLI G'TGCA_C
Cuts at: 2540 3740 4896 2540
Size: 1200 1156 3327
Fragments arranged by size:
3327 1200 1156
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 4459 618
Size: 325 217 627 1217 1455 1842
Fragments arranged by size:
1842 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2323 2394 3002 1391
Size: 932 71 608 4072
Fragments arranged by size:
4072 932 608 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2662 5678 2662
Size: 3016 2667
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2544 2594 3744 4900 1790
Size: 754 50 1150 1156 2573
Fragments arranged by size:
2573 1156 1150 754 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2932 3414 4372
Size: 416 53 83 399 24 1515 482 958
Cuts at: 4372 442
Size: 1753
Fragments arranged by size:
1753 1515 958 482 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 2309 2378 4343 1239
Size: 777 293 69 1965 2579
Fragments arranged by size:
2579 1965 777 293 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3808 4673 4971 2996
Size: 812 865 298 3708
Fragments arranged by size:
3708 865 812 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2813 4346 882
Size: 617 1314 1533 2219
Fragments arranged by size:
2219 1533 1314 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2170 1186
Size: 984 4699
BseYI C'CCAG_C
Cuts at: 2693 2966 4886 5382 2693
Size: 273 1920 496 2994
Fragments arranged by size:
2994 1920 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2192 2718 1163
Size: 573 345 111 526 4128
Fragments arranged by size:
4128 573 526 345 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2544 3744 3829 4900 2544
Size: 1200 85 1071 3327
Fragments arranged by size:
3327 1200 1071 85
BsmI GAATG_Cn'
Cuts at: 2287 2362 3194 3287 2287
Size: 75 832 93 4683
Fragments arranged by size:
4683 832 93 75
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 2394 2544 2594 3002 3744 3829
Size: 399 533 71 150 50 408 742 85
Cuts at: 3829 4900 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 533 408 399 150 85
71 50
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 2058 3572 5302 2058
Size: 1514 1730 2439
Fragments arranged by size:
2439 1730 1514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2752 3038 844
Size: 1908 286 3489
Fragments arranged by size:
3489 1908 286
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 3938
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 2665 2260
Size: 405 5278
BstYI r'GATC_y
Cuts at: 1972 2662 2956 3013 3380 3765 3782 5234 5320
Size: 690 294 57 367 385 17 1452 86
Cuts at: 5320 5332 5678 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 690 385 367 346 294 86
57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2688 513
Size: 552 1623 3508
Fragments arranged by size:
3508 1623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2219 3199 3993 4013 2219
Size: 980 794 20 3889
Fragments arranged by size:
3889 980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DraIII CAC_nnn'GTG
Cuts at: 2104 2104
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648
Size: 54 1425 50 275 18 1300 889 1629
Cuts at: 5648 8
Size: 43
Fragments arranged by size:
1629 1425 1300 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3613 1353
Size: 2260 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2309 2378 3746 4343 5130 5398
Size: 393 384 293 69 1368 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 1368 787 597 393 384 293 268
69
EcoNI CCTnn'n_nnAGG
Cuts at: 2627 2627
Size: 5683
EcoO109I rG'GnC_Cy
Cuts at: 2368 2708 2368
Size: 340 5343
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2497 4830 1678
Size: 819 2333 2531
Fragments arranged by size:
2531 2333 819
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 4324 4356 4398
Size: 32 135 32 605 32 2115 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 2115 605 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2455 2557 3099 3776 4924 5169 1606
Size: 849 102 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 849 677 542 245 102
NcoI C'CATG_G
Cuts at: 513 1065 2688 513
Size: 552 1623 3508
Fragments arranged by size:
3508 1623 552
NdeI CA'TA_TG
Cuts at: 387 2119 387
Size: 1732 3951
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2041 3041 4586 1132
Size: 97 812 1000 1545 2229
Fragments arranged by size:
2229 1545 1000 812 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2368 2368
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 2135 1613
Size: 522 5161
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2454 3035 830
Size: 1624 581 3478
Fragments arranged by size:
3478 1624 581
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2455 2557 2455
Size: 102 5581
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2324 2330 2450 3031 4166 4847 5038
Size: 219 1279 6 120 581 1135 681 191
Cuts at: 5038 826
Size: 1471
Fragments arranged by size:
1471 1279 1135 681 581 219 191 120
6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2975 3787 4688 4950 820
Size: 197 926 1032 812 901 262 1553
Fragments arranged by size:
1553 1032 926 901 812 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2160 2688 2826 3122 513
Size: 552 1095 528 138 296 3074
Fragments arranged by size:
3074 1095 552 528 296 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2226 3710 3727 3880 4065 4510 325
Size: 1255 646 1484 17 153 185 445 1498
Fragments arranged by size:
1498 1484 1255 646 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI