Restriction map for
pFN21AE6357
(Circular) (Six-base) MAPSORT of: pFN21AE6357.seq Check: 3146 from: 1 to: 5680
pFN21AE6357 5680 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2535 2608 2806 2535
Size: 73 198 5409
Fragments arranged by size:
5409 198 73
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2961 3023 1937
Size: 1024 62 4594
Fragments arranged by size:
4594 1024 62
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 3743 5127 5395 1632
Size: 1172 939 1384 268 1917
Fragments arranged by size:
1917 1384 1172 939 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 4297
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 2806 3001 4456 618
Size: 325 217 627 515 504 195 1455 1842
Fragments arranged by size:
1842 1455 627 515 504 325 217 195
BanII G_rGCy'C
Cuts at: 1391 2089 2888 2926 2999 1391
Size: 698 799 38 73 4072
Fragments arranged by size:
4072 799 698 73 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2357 928
Size: 971 458 4251
Fragments arranged by size:
4251 971 458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 2751 1548
Size: 1203 4477
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2307 2556 2809 2869 3741 4897 1790
Size: 350 167 249 253 60 872 1156 2573
Fragments arranged by size:
2573 1156 872 350 253 249 167 60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2502 2786 4340 1239
Size: 1263 284 1554 2579
Fragments arranged by size:
2579 1554 1263 284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2254 4113 4511 4785 4932 5343 2254
Size: 1859 398 274 147 411 2591
Fragments arranged by size:
2591 1859 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 2058 2452 2800 2945 4883 5379 2058
Size: 394 348 145 1938 496 2359
Fragments arranged by size:
2359 1938 496 394 348 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 1163
Size: 573 450 4657
Fragments arranged by size:
4657 573 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2888 2926 3741 3826 4897 2888
Size: 38 815 85 1071 3671
Fragments arranged by size:
3671 1071 815 85 38
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2140 2307 2556 2809 2869 2888
Size: 399 299 51 167 249 253 60 19
Cuts at: 2888 2926 2999 3741 3826 4897 1391
Size: 38 73 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 299 253 249 167
85 73 60 51 38 19
BspEI T'CCGG_A
Cuts at: 2254 5343 2254
Size: 3089 2591
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 2608 2636 2806 3035 844
Size: 1691 73 28 170 229 3489
Fragments arranged by size:
3489 1691 229 170 73 28
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 4324 4511 1700
Size: 110 370 2144 187 2869
Fragments arranged by size:
2869 2144 370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 2188 1184
Size: 1004 4676
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2500
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2928 3010 3377 3762 3779 5231 5317 5329
Size: 956 82 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 956 385 367 346 86 82
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 2493 2733 513
Size: 552 939 357 132 240 3460
Fragments arranged by size:
3460 939 552 357 240 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2381 2428 2571 3196 3990 4010 2381
Size: 47 143 625 794 20 4051
Fragments arranged by size:
4051 794 625 143 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2404 2439 529
Size: 35 1840 35 3770
Fragments arranged by size:
3770 1840 35 35
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2284 2490 2736
Size: 54 1425 50 275 18 454 206 246
Cuts at: 2736 3127 4016 5645 8
Size: 391 889 1629 43
Fragments arranged by size:
1629 1425 889 454 391 275 246 206
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2876 3610 1353
Size: 1523 734 3423
Fragments arranged by size:
3423 1523 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2233 4267 4641 4787 387
Size: 1064 138 644 2034 374 146 1280
Fragments arranged by size:
2034 1280 1064 644 374 146 138
EcoICRI GAG'CTC
Cuts at: 2886 2924 2886
Size: 38 5642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 2786 2804 3743 4340 5127 5395
Size: 393 870 284 18 939 597 787 268
Cuts at: 5395 1239
Size: 1524
Fragments arranged by size:
1524 939 870 787 597 393 284 268
18
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 5680
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2791 4827 1678
Size: 1113 2036 2531
Fragments arranged by size:
2531 2036 1113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2913 2945 4321 4353 4395
Size: 32 135 32 1341 32 1376 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1376 1341 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2407 3024 3270 669
Size: 988 281 469 617 246 3079
Fragments arranged by size:
3079 988 617 469 281 246
HindIII A'AGCT_T
Cuts at: 748 2148 748
Size: 1400 4280
HpaI GTT'AAC
Cuts at: 2407 3270 2407
Size: 863 4817
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 4794 4978 1305
Size: 1134 2355 184 2007
Fragments arranged by size:
2355 2007 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 2738 10
Size: 54 1425 343 660 246 2952
Fragments arranged by size:
2952 1425 660 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2441 2630 3638 3997 4156 518
Size: 648 1146 129 189 1008 359 159 2042
Fragments arranged by size:
2042 1146 1008 648 359 189 159 129
MspA1I CmG'CkG
Cuts at: 1606 2143 2215 2452 2573 3096 3773 4921 5166
Size: 537 72 237 121 523 677 1148 245
Cuts at: 5166 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 537 523 245 237 121
72
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 5680
NcoI C'CATG_G
Cuts at: 513 1065 2004 2733 513
Size: 552 939 729 3460
Fragments arranged by size:
3460 939 729 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2293 2335 3038 4583 1132
Size: 97 1064 42 703 1545 2229
Fragments arranged by size:
2229 1545 1064 703 97 42
PasI CC'CwG_GG
Cuts at: 1348 2195 2500 1348
Size: 847 305 4528
Fragments arranged by size:
4528 847 305
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2619 2677 2817 3032 830
Size: 1789 58 140 215 3478
Fragments arranged by size:
3478 1789 215 140 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2143 2215 2452 2573 2143
Size: 72 237 121 5250
Fragments arranged by size:
5250 237 121 72
SacI G_AGCT'C
Cuts at: 2888 2926 2888
Size: 38 5642
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2876 2876
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 2817 3032 2817
Size: 215 5465
ScaI AGT'ACT
Cuts at: 2071 3928 5640 2071
Size: 1857 1712 2111
Fragments arranged by size:
2111 1857 1712
SfcI C'TryA_G
Cuts at: 826 1045 2173 2344 2615 2673 2813 3028 4163
Size: 219 1128 171 271 58 140 215 1135
Cuts at: 4163 4844 5035 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 271 219 215 191
171 140 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 2335 3038 1132
Size: 1203 703 3774
Fragments arranged by size:
3774 1203 703
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2535 2733 3119 513
Size: 552 939 531 198 386 3074
Fragments arranged by size:
3074 939 552 531 386 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2009 2368 3707 3724 3877 4062 4507
Size: 1255 429 359 1339 17 153 185 445
Cuts at: 4507 325
Size: 1498
Fragments arranged by size:
1498 1339 1255 445 429 359 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 3926 5638
Size: 274 80 33 51 1443 92 1857 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1857 1712 1443 274 138 92 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 5640 5672 2201
Size: 32 3407 32 2209
Fragments arranged by size:
3407 2209 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2953 2485
Size: 468 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3809 2777
Size: 1032 4648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI