Restriction map for
pFN21AE6354
(Circular) (Six-base) MAPSORT of: pFN21AE6354.seq Check: 8074 from: 1 to: 5860
pFN21AE6354 5860 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2054 3670 278
Size: 53 83 186 936 518 1616 2468
Fragments arranged by size:
2468 1616 936 518 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2298 3203 1937
Size: 361 905 4594
Fragments arranged by size:
4594 905 361
Acc65I G'GTAC_C
Cuts at: 2577 2970 3181 2577
Size: 393 211 5256
Fragments arranged by size:
5256 393 211
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 3923 5307 5575 1632
Size: 675 1616 1384 268 1917
Fragments arranged by size:
1917 1616 1384 675 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 2415 1166
Size: 1249 4611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2684 1184
Size: 1500 4360
AlwNI CAG_nnn'CTG
Cuts at: 2248 2285 3088 3603 5175 2248
Size: 37 803 515 1572 2933
Fragments arranged by size:
2933 1572 803 515 37
ApaI G_GGCC'C
Cuts at: 2587 2587
Size: 5860
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 3185 1870
Size: 73 191 1051 4545
Fragments arranged by size:
4545 1051 191 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2072 2423 2577 2970
Size: 325 217 627 229 56 351 154 393
Cuts at: 2970 3181 4636 618
Size: 211 1455 1842
Fragments arranged by size:
1842 1455 627 393 351 325 229 217
211 154 56
BanII G_rGCy'C
Cuts at: 1391 2587 2943 3179 1391
Size: 1196 356 236 4072
Fragments arranged by size:
4072 1196 356 236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BbvCI CC'TCA_GC
Cuts at: 2099 3140 2099
Size: 1041 4819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BfrBI ATG'CAT
Cuts at: 2569 2569
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 2688 4471
Size: 107 122 71 631 605 466 550 1783
Cuts at: 4471 136
Size: 1525
Fragments arranged by size:
1783 1525 631 605 550 466 122 107
71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2587 2966 3921 5077 1790
Size: 287 510 379 955 1156 2573
Fragments arranged by size:
2573 1156 955 510 379 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 2434 3591
Size: 416 53 83 399 24 856 161 1157
Cuts at: 3591 4549 442
Size: 958 1753
Fragments arranged by size:
1753 1157 958 856 416 399 161 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 2955 4520 1239
Size: 1301 415 1565 2579
Fragments arranged by size:
2579 1565 1301 415
Bpu10I CC'TnA_GC
Cuts at: 2099 3140 2099
Size: 1041 4819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 2624 2636 3985 4850 5148 2618
Size: 6 12 1349 865 298 3330
Fragments arranged by size:
3330 1349 865 298 12 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2261 3046 4523 882
Size: 617 762 785 1477 2219
Fragments arranged by size:
2219 1477 785 762 617
BsaAI yAC'GTr
Cuts at: 493 1204 2668 2967 3651 493
Size: 711 1464 299 684 2702
Fragments arranged by size:
2702 1464 711 684 299
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 3667 4049 4684
Size: 53 83 186 936 518 1616 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1616 1451 936 635 518 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2775 4293 4691 4965 5112 5523 2775
Size: 1518 398 274 147 411 3112
Fragments arranged by size:
3112 1518 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2981 1178
Size: 30 165 30 1548 30 4057
Fragments arranged by size:
4057 1548 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 3034 1186
Size: 1435 413 4012
Fragments arranged by size:
4012 1435 413
BseYI C'CCAG_C
Cuts at: 2115 2586 2736 2991 5063 5559 2115
Size: 471 150 255 2072 496 2416
Fragments arranged by size:
2416 2072 496 471 255 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2707 3018 1163
Size: 573 971 311 4005
Fragments arranged by size:
4005 971 573 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2556 3921 4006 5077 2556
Size: 1365 85 1071 3339
Fragments arranged by size:
3339 1365 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2556 2587 2943 2966 3179 3921
Size: 399 287 479 31 356 23 213 742
Cuts at: 3921 4006 5077 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 479 399 356 287 213
85 31 23
BspEI T'CCGG_A
Cuts at: 2775 5523 2775
Size: 2748 3112
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2565 3215 844
Size: 1255 466 650 3489
Fragments arranged by size:
3489 1255 650 466
BsrBI CCG'CTC
Cuts at: 1394 1823 2310 3747 1394
Size: 429 487 1437 3507
Fragments arranged by size:
3507 1437 487 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 4355 4529 65
Size: 1183 1182 1925 174 1396
Fragments arranged by size:
1925 1396 1183 1182 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 2208 2420 4504 4691 1700
Size: 110 259 139 212 2084 187 2869
Fragments arranged by size:
2869 2084 259 212 187 139 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 5755
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 3060 3190 3557 3942 3959 5411 5497 5509
Size: 1088 130 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1088 385 367 346 130 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2345 2345
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 2361 2510 2955 513
Size: 552 939 258 99 149 445 3418
Fragments arranged by size:
3418 939 552 445 258 149 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2246 2461 3131 3376 4170 4190 2148
Size: 98 215 670 245 794 20 3818
Fragments arranged by size:
3818 794 670 245 215 98 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2171 4867 809
Size: 507 855 2696 1802
Fragments arranged by size:
2696 1802 855 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825
Size: 54 1425 50 275 18 1477 889 1629
Cuts at: 5825 8
Size: 43
Fragments arranged by size:
1629 1477 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3005 3790 1353
Size: 1652 785 3423
Fragments arranged by size:
3423 1652 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2540 2955 3923 4520 5307 5575
Size: 393 675 233 415 968 597 787 268
Cuts at: 5575 1239
Size: 1524
Fragments arranged by size:
1524 968 787 675 597 415 393 268
233
EcoO109I rG'GnC_Cy
Cuts at: 2335 2350 3019 3085 3111 2335
Size: 15 669 66 26 5084
Fragments arranged by size:
5084 669 66 26 15
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2044 2144 5007 1678
Size: 366 100 2863 2531
Fragments arranged by size:
2863 2531 366 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373
Size: 32 135 32 2929 32 42 32 2626
Fragments arranged by size:
2929 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2299 3204 3450 669
Size: 988 281 361 905 246 3079
Fragments arranged by size:
3079 988 905 361 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 2581 2974 3185 2581
Size: 393 211 5256
Fragments arranged by size:
5256 393 211
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4038
Fragments arranged by size:
4038 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2415 3818 4177 4336 518
Size: 648 1249 1403 359 159 2042
Fragments arranged by size:
2042 1403 1249 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2590 2721 3276 3953 5101 5346 1606
Size: 984 131 555 677 1148 245 2120
Fragments arranged by size:
2120 1148 984 677 555 245 131
NaeI GCC'GGC
Cuts at: 2210 2422 2210
Size: 212 5648
NcoI C'CATG_G
Cuts at: 513 1065 2004 2262 2955 513
Size: 552 939 258 693 3418
Fragments arranged by size:
3418 939 693 552 258
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2208 2420 2208
Size: 212 5648
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NsiI A_TGCA'T
Cuts at: 2571 2571
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2764 3218 4763 1132
Size: 97 1535 454 1545 2229
Fragments arranged by size:
2229 1545 1535 454 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 3107 3107
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PmlI CAC'GTG
Cuts at: 2967 2967
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2350 3111 2335
Size: 15 761 5084
Fragments arranged by size:
5084 761 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2583 2583
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2110 3212 830
Size: 1280 1102 3478
Fragments arranged by size:
3478 1280 1102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 2297 3202 1936
Size: 361 905 4594
Fragments arranged by size:
4594 905 361
SanDI GG'GwC_CC
Cuts at: 2350 2350
Size: 5860
SapI GCTCTTCn'nnn_
Cuts at: 3005 3005
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SexAI A'CCwGG_T
Cuts at: 2973 2973
Size: 5860
SfcI C'TryA_G
Cuts at: 826 1045 2106 3035 3208 4343 5024 5215 826
Size: 219 1061 929 173 1135 681 191 1471
Fragments arranged by size:
1471 1135 1061 929 681 219 191 173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 2069 1700
Size: 369 5491
SmaI CCC'GGG
Cuts at: 1872 2136 3187 1872
Size: 264 1051 4545
Fragments arranged by size:
4545 1051 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 2639 2651 3964 4865 5127
Size: 197 926 690 6 12 1313 901 262
Cuts at: 5127 820
Size: 1553
Fragments arranged by size:
1553 1313 926 901 690 262 197 12
6
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2262 2748 2943 2955 3299
Size: 552 939 183 75 486 195 12 344
Cuts at: 3299 513
Size: 3074
Fragments arranged by size:
3074 939 552 486 344 195 183 75
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2434 2593 2918 3040 3887 3904
Size: 1255 675 179 159 325 122 847 17
Cuts at: 3904 4057 4242 4687 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 847 675 445 325 185 179
159 153 122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 4106 5818
Size: 274 80 33 51 1443 678 1451 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1451 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2140 2172 2951 2983 5820 5852 2140
Size: 32 779 32 2837 32 2148
Fragments arranged by size:
2837 2148 779 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 2134 3185 1870
Size: 264 1051 4545
Fragments arranged by size:
4545 1051 264
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2052 3668 276
Size: 53 83 186 936 518 1616 2468
Fragments arranged by size:
2468 1616 936 518 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI PacI PspXI
PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI
SwaI XcmI