Restriction map for
pFN21AE6352
(Circular) (Six-base) MAPSORT of: pFN21AE6352.seq Check: 128 from: 1 to: 5815
pFN21AE6352 5815 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2011 2011
Size: 5815
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3878 5262 5530 1632
Size: 2246 1384 268 1917
Fragments arranged by size:
2246 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 2448 2532 4714 5691 1203
Size: 1245 84 2182 977 1327
Fragments arranged by size:
2182 1327 1245 977 84
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 2630 1166
Size: 1464 4351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 3073 3558 5130 3073
Size: 485 1572 3758
Fragments arranged by size:
3758 1572 485
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 2403 3092 3124 3350 3502 5611 1493
Size: 910 689 32 226 152 2109 1697
Fragments arranged by size:
2109 1697 910 689 226 152 32
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 2792 3136 4591 618
Size: 325 217 627 807 198 344 1455 1842
Fragments arranged by size:
1842 1455 807 627 344 325 217 198
BanII G_rGCy'C
Cuts at: 1391 2889 3134 1391
Size: 1498 245 4072
Fragments arranged by size:
4072 1498 245
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3066 928
Size: 971 1167 3677
Fragments arranged by size:
3677 1167 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BfrBI ATG'CAT
Cuts at: 3106 3106
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 2813 3232 2813
Size: 419 5396
Bme1580I G_kGCm'C
Cuts at: 1790 2599 2678 3876 5032 1790
Size: 809 79 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 809 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2198 1056
Size: 1142 4673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2789 4475 1239
Size: 1550 1686 2579
Fragments arranged by size:
2579 1686 1550
Bpu10I CC'TnA_GC
Cuts at: 2331 2331
Size: 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2844 3940 4805 5103 2844
Size: 1096 865 298 3556
Fragments arranged by size:
3556 1096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2449 3606 493
Size: 711 1245 1157 2702
Fragments arranged by size:
2702 1245 1157 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 3622 4004 4639
Size: 53 83 186 936 477 1612 382 635
Cuts at: 4639 275
Size: 1451
Fragments arranged by size:
1612 1451 936 635 477 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5815
BseYI C'CCAG_C
Cuts at: 2618 2653 5018 5514 2618
Size: 35 2365 496 2919
Fragments arranged by size:
2919 2365 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 1163
Size: 573 984 4258
Fragments arranged by size:
4258 984 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3876 3961 5032 3876
Size: 85 1071 4659
Fragments arranged by size:
4659 1071 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
BsmBI CGTCTCn'nnnn_
Cuts at: 2462 2462
Size: 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2599 2678 2889 3134 3876 3961 5032
Size: 399 809 79 211 245 742 85 1071
Cuts at: 5032 1391
Size: 2174
Fragments arranged by size:
2174 1071 809 742 399 245 211 85
79
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3084 3704 5434 3084
Size: 620 1730 3465
Fragments arranged by size:
3465 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2011 2658 3170 844
Size: 1167 647 512 3489
Fragments arranged by size:
3489 1167 647 512
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2935 4310 4484 65
Size: 1183 1687 1375 174 1396
Fragments arranged by size:
1687 1396 1375 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 2721 96
Size: 2625 3190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 2623 1184
Size: 1439 4376
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2421 2560 2023
Size: 398 139 5278
Fragments arranged by size:
5278 398 139
BstYI r'GATC_y
Cuts at: 1972 2410 3145 3512 3897 3914 5366 5452 5464
Size: 438 735 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 735 438 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2628 2796 2802 513
Size: 552 939 624 168 6 3526
Fragments arranged by size:
3526 939 624 552 168 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 4125 4145 3331
Size: 794 20 5001
Fragments arranged by size:
5001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DraIII CAC_nnn'GTG
Cuts at: 2065 2065
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 2799 3075
Size: 54 1425 50 275 18 415 554 276
Cuts at: 3075 3262 4151 5780 8
Size: 187 889 1629 43
Fragments arranged by size:
1629 1425 889 554 415 276 275 187
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3745 1353
Size: 2392 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2789 3878 4475 5262 5530 1239
Size: 393 1157 1089 597 787 268 1524
Fragments arranged by size:
1524 1157 1089 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FspI TGC'GCA
Cuts at: 2461 4321 2461
Size: 1860 3955
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 4456 4488 4530
Size: 32 135 32 950 32 1902 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 1902 950 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 3159 3405 669
Size: 988 281 91 1130 246 3079
Fragments arranged by size:
3079 1130 988 281 246 91
HindIII A'AGCT_T
Cuts at: 748 2810 748
Size: 2062 3753
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3054 3414 3054
Size: 360 5455
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4929 5113 1305
Size: 3624 184 2007
Fragments arranged by size:
3624 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3773 4132 4291 518
Size: 648 1464 1143 359 159 2042
Fragments arranged by size:
2042 1464 1143 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2798 3231 3908 5056 5301 1606
Size: 1192 433 677 1148 245 2120
Fragments arranged by size:
2120 1192 1148 677 433 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2802 513
Size: 552 939 798 3526
Fragments arranged by size:
3526 939 798 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 2194 1052
Size: 1142 4673
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NsiI A_TGCA'T
Cuts at: 3108 3108
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2536 3173 4718 1132
Size: 97 1307 637 1545 2229
Fragments arranged by size:
2229 1545 1307 637 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 2532 4714 2532
Size: 2182 3633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PfoI T'CCnGG_A
Cuts at: 2685 2685
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
SacII CC_GC'GG
Cuts at: 2799 2799
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 2520 4063 5775 2520
Size: 1543 1712 2560
Fragments arranged by size:
2560 1712 1543
SfcI C'TryA_G
Cuts at: 826 1045 3163 4298 4979 5170 826
Size: 219 2118 1135 681 191 1471
Fragments arranged by size:
2118 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2859 3919 4820 5082 820
Size: 197 926 916 1060 901 262 1553
Fragments arranged by size:
1553 1060 926 916 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2911 3739 5
Size: 47 2859 828 2081
Fragments arranged by size:
2859 2081 828 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 2802 3254 513
Size: 552 939 798 452 3074
Fragments arranged by size:
3074 939 798 552 452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2229 2671 3842 3859 4012 4197 4642
Size: 1255 649 442 1171 17 153 185 445
Cuts at: 4642 325
Size: 1498
Fragments arranged by size:
1498 1255 1171 649 445 442 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2518 2721 4061
Size: 274 80 33 51 1443 541 203 1340
Cuts at: 4061 5773 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1340 541 274 203 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2606 2606
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3063 3944 3063
Size: 881 4934
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI