Restriction map for
pFN21AE6348
(Circular) (Six-base) MAPSORT of: pFN21AE6348.seq Check: 2236 from: 1 to: 5698
pFN21AE6348 5698 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2527 2527
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2297 3508 278
Size: 53 83 186 936 761 1211 2468
Fragments arranged by size:
2468 1211 936 761 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2505 3041 1937
Size: 568 536 4594
Fragments arranged by size:
4594 568 536
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 2321 3761 5145 5413 1632
Size: 650 39 1440 1384 268 1917
Fragments arranged by size:
1917 1440 1384 650 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 4501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2985 1184
Size: 1801 3897
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 5666
AlwNI CAG_nnn'CTG
Cuts at: 2211 3441 5013 2211
Size: 1230 1572 2896
Fragments arranged by size:
2896 1572 1230
ApaI G_GGCC'C
Cuts at: 2029 2922 2029
Size: 893 4805
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 3023 1870
Size: 73 376 704 4545
Fragments arranged by size:
4545 704 376 73
BamHI G'GATC_C
Cuts at: 2490 3028 3395 2490
Size: 538 367 4793
Fragments arranged by size:
4793 538 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2064 2774 2840 3019
Size: 325 217 627 261 16 710 66 179
Cuts at: 3019 4474 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 710 627 325 261 217 179
66 16
BanII G_rGCy'C
Cuts at: 1391 2029 2193 2553 2922 3017 1391
Size: 638 164 360 369 95 4072
Fragments arranged by size:
4072 638 369 360 164 95
BbeI G_GCGC'C
Cuts at: 2052 2068 2778 2052
Size: 16 710 4972
Fragments arranged by size:
4972 710 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2762 4309 136
Size: 107 122 71 631 605 1090 1547 1525
Fragments arranged by size:
1547 1525 1090 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2460 5693 2460
Size: 3233 2465
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2845 2922 3759 4915 1790
Size: 239 816 77 837 1156 2573
Fragments arranged by size:
2573 1156 837 816 239 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2309 2900 2909 2945 4358 1239
Size: 893 177 591 9 36 1413 2579
Fragments arranged by size:
2579 1413 893 591 177 36 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 4361 882
Size: 617 663 2199 2219
Fragments arranged by size:
2219 2199 663 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2065 2294 2775
Size: 53 83 186 936 516 16 229 481
Cuts at: 2775 2943 3505 3887 4522 275
Size: 168 562 382 635 1451
Fragments arranged by size:
1451 936 635 562 516 481 382 229
186 168 83 53 16
BsaWI w'CCGG_w
Cuts at: 2277 2808 4131 4529 4803 4950 5361 2277
Size: 531 1323 398 274 147 411 2614
Fragments arranged by size:
2614 1323 531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4450
Fragments arranged by size:
4450 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5698
BseYI C'CCAG_C
Cuts at: 2044 2197 2606 2786 4901 5397 2044
Size: 153 409 180 2115 496 2345
Fragments arranged by size:
2345 2115 496 409 180 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 4480
Fragments arranged by size:
4480 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 2724 3759 3844 4915 2553
Size: 171 1035 85 1071 3336
Fragments arranged by size:
3336 1071 1035 171 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 2285
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2193 2553 2724 2845 2922 3017
Size: 399 239 164 360 171 121 77 95
Cuts at: 3017 3759 3844 4915 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 239 171 164
121 95 85 77
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2527 3053 844
Size: 1502 181 526 3489
Fragments arranged by size:
3489 1502 526 181
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2736 4193 4367 65
Size: 1183 1488 1457 174 1396
Fragments arranged by size:
1488 1457 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2621 2929 4342 4529 1700
Size: 110 811 308 1413 187 2869
Fragments arranged by size:
2869 1413 811 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 2148 2517 2697 96
Size: 2052 369 180 3097
Fragments arranged by size:
3097 2052 369 180
BssHII G'CGCG_C
Cuts at: 1925 2101 1925
Size: 176 5522
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2211 794
Size: 1417 4281
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2059 2044
Size: 15 5683
BstYI r'GATC_y
Cuts at: 1972 2460 2490 3028 3395 3780 3797 5249 5335
Size: 488 30 538 367 385 17 1452 86
Cuts at: 5335 5347 5693 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 538 488 385 367 346 86
30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2564 2564
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 2037 2052 2661 2845 513
Size: 552 972 15 609 184 3366
Fragments arranged by size:
3366 972 609 552 184 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2318 2375 3214 4008 4028 2318
Size: 57 839 794 20 3988
Fragments arranged by size:
3988 839 794 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DraIII CAC_nnn'GTG
Cuts at: 2381 2381
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 2119 2454
Size: 54 1425 50 275 18 200 89 335
Cuts at: 2454 2799 3145 4034 5663 8
Size: 345 346 889 1629 43
Fragments arranged by size:
1629 1425 889 346 345 335 275 200
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 2364 2558 3628 1353
Size: 983 28 194 1070 3423
Fragments arranged by size:
3423 1070 983 194 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2551
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2282 2309 2321 2900 2909 2945
Size: 393 500 150 27 12 579 9 36
Cuts at: 2945 3761 4358 5145 5413 1239
Size: 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 579 500 393 268
150 36 27 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2563 2563
Size: 5698
EcoO109I rG'GnC_Cy
Cuts at: 2611 2755 2918 2919 2611
Size: 144 163 1 5390
Fragments arranged by size:
5390 163 144 1
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2052 2068 2091 2778 2784 4845 1678
Size: 374 16 23 687 6 2061 2531
Fragments arranged by size:
2531 2061 687 374 23 16 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 4339 4371 4413
Size: 32 135 32 575 32 2160 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 2160 575 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KasI G'GCGC_C
Cuts at: 2048 2064 2774 2048
Size: 16 710 4972
Fragments arranged by size:
4972 710 16
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 4812 4996 1305
Size: 1152 2355 184 2007
Fragments arranged by size:
2355 2007 1152 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2363 3656 4015 4174 518
Size: 648 891 306 1293 359 159 2042
Fragments arranged by size:
2042 1293 891 648 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2287 2395 2833 2887 2906 3114 3791
Size: 411 270 108 438 54 19 208 677
Cuts at: 3791 4939 5184 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 438 411 270 245 208
108 54 19
NaeI GCC'GGC
Cuts at: 2931 2931
Size: 5698
NarI GG'CG_CC
Cuts at: 2049 2065 2775 2049
Size: 16 710 4972
Fragments arranged by size:
4972 710 16
NcoI C'CATG_G
Cuts at: 513 1065 2052 513
Size: 552 987 4159
Fragments arranged by size:
4159 987 552
NdeI CA'TA_TG
Cuts at: 387 2343 387
Size: 1956 3742
NgoMIV G'CCGG_C
Cuts at: 2929 2929
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2518 2872 3056 4601 1132
Size: 97 1289 354 184 1545 2229
Fragments arranged by size:
2229 1545 1289 354 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2249 1108
Size: 1141 4557
PfoI T'CCnGG_A
Cuts at: 2109 2109
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2428 3767 3799 5280 5312 2396
Size: 32 1339 32 1481 32 2782
Fragments arranged by size:
2782 1481 1339 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2025 2918 2025
Size: 893 4805
PstI C_TGCA'G
Cuts at: 830 2216 2313 2763 2838 3050 830
Size: 1386 97 450 75 212 3478
Fragments arranged by size:
3478 1386 450 212 97 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2017 2287 2395 2833 2887 2017
Size: 270 108 438 54 4828
Fragments arranged by size:
4828 438 270 108 54
SacI G_AGCT'C
Cuts at: 2553 2553
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2558 2558
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 2763 3050 2763
Size: 287 5411
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2212 2309 2759 2834 3046 4181 4862
Size: 219 1167 97 450 75 212 1135 681
Cuts at: 4862 5053 826
Size: 191 1471
Fragments arranged by size:
1471 1167 1135 681 450 219 212 191
97 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 2762 2762
Size: 5698
SfoI GGC'GCC
Cuts at: 2050 2066 2776 2050
Size: 16 710 4972
Fragments arranged by size:
4972 710 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2052 2176 2232 3137 513
Size: 552 987 124 56 905 3074
Fragments arranged by size:
3074 987 905 552 124 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2517 2628
Size: 274 80 33 51 1443 171 369 111
Cuts at: 2628 2697 3944 5656 96
Size: 69 1247 1712 138
Fragments arranged by size:
1712 1443 1247 369 274 171 138 111
80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 2855 1316
Size: 1539 4159
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2295 3506 276
Size: 53 83 186 936 761 1211 2468
Fragments arranged by size:
2468 1211 936 761 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SrfI SwaI XcmI