Restriction map for
pFN21AE6347
(Circular) (Six-base) MAPSORT of: pFN21AE6347.seq Check: 4868 from: 1 to: 5686
pFN21AE6347 5686 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2130 3496 278
Size: 53 83 186 936 594 1366 2468
Fragments arranged by size:
2468 1366 936 594 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 2480 2649 2714 2758 2903 3749 5133
Size: 509 339 169 65 44 145 846 1384
Cuts at: 5133 5401 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 846 509 339 268 169 145
65 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 2065 2641 3429 5001 2065
Size: 576 788 1572 2750
Fragments arranged by size:
2750 1572 788 576
ApaI G_GGCC'C
Cuts at: 2596 2596
Size: 5686
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 2188 2820 3011 1870
Size: 73 158 87 632 191 4545
Fragments arranged by size:
4545 632 191 158 87 73
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 2937 3007 4462 618
Size: 325 217 627 371 779 70 1455 1842
Fragments arranged by size:
1842 1455 779 627 371 325 217 70
BanII G_rGCy'C
Cuts at: 1391 2596 2842 3005 1391
Size: 1205 246 163 4072
Fragments arranged by size:
4072 1205 246 163
BbeI G_GCGC'C
Cuts at: 2162 2162
Size: 5686
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2786 928
Size: 971 887 3828
Fragments arranged by size:
3828 971 887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2186 3578 4788 2186
Size: 1392 1210 3084
Fragments arranged by size:
3084 1392 1210
BclI T'GATC_A
Cuts at: 1548 2742 1548
Size: 1194 4492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 2472 3103 2472
Size: 631 5055
Bme1580I G_kGCm'C
Cuts at: 1790 2596 3747 4903 1790
Size: 806 1151 1156 2573
Fragments arranged by size:
2573 1156 1151 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2898 1056
Size: 1842 3844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 4346 1239
Size: 927 2180 2579
Fragments arranged by size:
2579 2180 927
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2142 4349 882
Size: 617 643 2207 2219
Fragments arranged by size:
2219 2207 643 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2127 2159 3493
Size: 53 83 186 936 516 78 32 1334
Cuts at: 3493 3875 4510 275
Size: 382 635 1451
Fragments arranged by size:
1451 1334 936 635 516 382 186 83
78 53 32
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2139 2169 2970 3000 1178
Size: 30 165 30 736 30 801 30 3864
Fragments arranged by size:
3864 801 736 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2235 2331 1186
Size: 1049 96 4541
Fragments arranged by size:
4541 1049 96
BseYI C'CCAG_C
Cuts at: 2293 2365 4889 5385 2293
Size: 72 2524 496 2594
Fragments arranged by size:
2594 2524 496 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2464 3747 3832 4903 2464
Size: 1283 85 1071 3247
Fragments arranged by size:
3247 1283 1071 85
BsmI GAATG_Cn'
Cuts at: 2764 3197 3290 2764
Size: 433 93 5160
Fragments arranged by size:
5160 433 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 2596 2842 3005 3747 3832 4903
Size: 399 674 132 246 163 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 674 399 246 163 132
85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 2863 3575 5305 2863
Size: 712 1730 3244
Fragments arranged by size:
3244 1730 712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 3573 1394
Size: 429 251 1499 3507
Fragments arranged by size:
3507 1499 429 251
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 4330 4517 1700
Size: 110 800 1720 187 2869
Fragments arranged by size:
2869 1720 800 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 3016 3383 3768 3785 5237 5323 5335 5681
Size: 1044 367 385 17 1452 86 12 346
Cuts at: 5681 1972
Size: 1977
Fragments arranged by size:
1977 1452 1044 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4195
Fragments arranged by size:
4195 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2976 487
Size: 683 56 1750 3197
Fragments arranged by size:
3197 1750 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3996 4016 3202
Size: 794 20 4872
Fragments arranged by size:
4872 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DraIII CAC_nnn'GTG
Cuts at: 2644 2644
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2790 4693 809
Size: 507 1474 1903 1802
Fragments arranged by size:
1903 1802 1474 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2325 3133
Size: 54 1425 50 275 18 226 269 808
Cuts at: 3133 4022 5651 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 808 275 269 226 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 2777 3616 1353
Size: 1272 152 839 3423
Fragments arranged by size:
3423 1272 839 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2540 4273 4647 4793 387
Size: 1064 138 951 1733 374 146 1280
Fragments arranged by size:
1733 1280 1064 951 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2166 2480 2649 2714 2758 2903
Size: 393 509 25 314 169 65 44 145
Cuts at: 2903 3749 4346 5133 5401 1239
Size: 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 597 509 393 314 268
169 145 65 44 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2645 2645
Size: 5686
EcoO109I rG'GnC_Cy
Cuts at: 2503 2592 2593 2767 2503
Size: 89 1 174 5422
Fragments arranged by size:
5422 174 89 1
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 5686
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2162 4833 1678
Size: 484 2671 2531
Fragments arranged by size:
2671 2531 484
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2794 2826 4327 4359 4401
Size: 32 135 32 1222 32 1501 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 1501 1222 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2657 748
Size: 1909 3777
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KasI G'GCGC_C
Cuts at: 2158 2158
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 10
Size: 54 1425 343 495 3369
Fragments arranged by size:
3369 1425 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 4003 4162 518
Size: 648 2478 359 159 2042
Fragments arranged by size:
2478 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2471 3102 3779 4927 5172 1606
Size: 865 631 677 1148 245 2120
Fragments arranged by size:
2120 1148 865 677 631 245
NaeI GCC'GGC
Cuts at: 2612 2612
Size: 5686
NarI GG'CG_CC
Cuts at: 2159 2159
Size: 5686
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4195
Fragments arranged by size:
4195 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NgoMIV G'CCGG_C
Cuts at: 2610 2610
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 2894 1052
Size: 1842 3844
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2311 2947 3044 4589 1132
Size: 97 1082 636 97 1545 2229
Fragments arranged by size:
2229 1545 1082 636 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PfoI T'CCnGG_A
Cuts at: 2034 2430 2034
Size: 396 5290
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2503
Size: 5686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspOMI G'GGCC_C
Cuts at: 2592 2592
Size: 5686
PstI C_TGCA'G
Cuts at: 830 3038 830
Size: 2208 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2471 2471
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SapI GCTCTTCn'nnn_
Cuts at: 2625 2625
Size: 5686
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SexAI A'CCwGG_T
Cuts at: 2640 2640
Size: 5686
SfcI C'TryA_G
Cuts at: 826 1045 2970 3034 4169 4850 5041 826
Size: 219 1925 64 1135 681 191 1471
Fragments arranged by size:
1925 1471 1135 681 219 191 64
SfoI GGC'GCC
Cuts at: 2160 2160
Size: 5686
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 2610 1700
Size: 910 4776
SmaI CCC'GGG
Cuts at: 1872 2822 3013 1872
Size: 950 191 4545
Fragments arranged by size:
4545 950 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 2311 3044 1132
Size: 1179 733 3774
Fragments arranged by size:
3774 1179 733
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2004 2763 3125 513
Size: 552 939 759 362 3074
Fragments arranged by size:
3074 939 759 552 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2718 3713 3730 3883 4068 4513 325
Size: 1255 1138 995 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1138 995 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 5646 5678 2139
Size: 32 3475 32 2147
Fragments arranged by size:
3475 2147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 2820 3011 1870
Size: 950 191 4545
Fragments arranged by size:
4545 950 191
XmnI GAAnn'nnTTC
Cuts at: 2561 3815 2561
Size: 1254 4432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2128 3494 276
Size: 53 83 186 936 594 1366 2468
Fragments arranged by size:
2468 1366 936 594 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI
XcmI