Restriction map for
pFN21AE6344
(Circular) (Six-base) MAPSORT of: pFN21AE6344.seq Check: 7931 from: 1 to: 5770
pFN21AE6344 5770 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2892 2892
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2039 3580 278
Size: 53 83 186 936 503 1541 2468
Fragments arranged by size:
2468 1541 936 503 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2286 2479 2852 3086 3833 5217 5485
Size: 410 244 193 373 234 747 1384 268
Cuts at: 5485 1632
Size: 1917
Fragments arranged by size:
1917 1384 747 410 373 268 244 234
193
AfeI AGC'GCT
Cuts at: 2486 2486
Size: 5770
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 3041 1166
Size: 1875 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2438 1184
Size: 1254 4516
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2085 2351 3079 3305 3457 5566 1493
Size: 592 266 728 226 152 2109 1697
Fragments arranged by size:
2109 1697 728 592 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 3095 1870
Size: 73 106 1046 4545
Fragments arranged by size:
4545 1046 106 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 3091 4546 618
Size: 325 217 627 279 1025 1455 1842
Fragments arranged by size:
1842 1455 1025 627 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2968 3089 1391
Size: 1577 121 4072
Fragments arranged by size:
4072 1577 121
BbeI G_GCGC'C
Cuts at: 2070 2070
Size: 5770
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2849 2883 3961 3995 1903
Size: 34 912 34 1078 34 3678
Fragments arranged by size:
3678 1078 912 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2634 3831 4987 1790
Size: 844 1197 1156 2573
Fragments arranged by size:
2573 1197 1156 844
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 4430 1239
Size: 1295 1896 2579
Fragments arranged by size:
2579 1896 1295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2778 2840 3895 4760 5058 2778
Size: 62 1055 865 298 3490
Fragments arranged by size:
3490 1055 865 298 62
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 2621 5764 2621
Size: 3143 2627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 2067 3577 3959
Size: 53 83 186 936 503 31 1510 382
Cuts at: 3959 4594 275
Size: 635 1451
Fragments arranged by size:
1510 1451 936 635 503 382 186 83
53 31
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4411
Fragments arranged by size:
4411 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5770
BseYI C'CCAG_C
Cuts at: 2070 4973 5469 2070
Size: 2903 496 2371
Fragments arranged by size:
2903 2371 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 1163
Size: 573 384 4813
Fragments arranged by size:
4813 573 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 2968 3831 3916 4987 2451
Size: 517 863 85 1071 3234
Fragments arranged by size:
3234 1071 863 517 85
BsmI GAATG_Cn'
Cuts at: 2725 2931 3281 3374 2725
Size: 206 350 93 5121
Fragments arranged by size:
5121 350 206 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2634 2968 3089 3831 3916 4987
Size: 399 661 183 334 121 742 85 1071
Cuts at: 4987 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 661 399 334 183 121
85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 2892 3125 844
Size: 1763 285 233 3489
Fragments arranged by size:
3489 1763 285 233
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2380 4265 4439 65
Size: 1183 1132 1885 174 1396
Fragments arranged by size:
1885 1396 1183 1132 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 2409 2523 96
Size: 2313 114 3343
Fragments arranged by size:
3343 2313 114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2671 3057 3100 3467 3852 3869 5321 5407
Size: 699 386 43 367 385 17 1452 86
Cuts at: 5407 5419 5765 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 699 386 385 367 346 86
43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2849 513
Size: 552 939 845 3434
Fragments arranged by size:
3434 939 845 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2534 487
Size: 683 56 1308 3723
Fragments arranged by size:
3723 1308 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 2108 2555 3286 4080 4100 2101
Size: 7 447 731 794 20 3771
Fragments arranged by size:
3771 794 731 447 20 7
ClaI AT'CG_AT
Cuts at: 1554 2625 1554
Size: 1071 4699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2790 2825 529
Size: 35 2226 35 3474
Fragments arranged by size:
3474 2226 35 35
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DraIII CAC_nnn'GTG
Cuts at: 2614 2614
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2041 4777 809
Size: 507 725 2736 1802
Fragments arranged by size:
2736 1802 725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735
Size: 54 1425 50 275 18 1387 889 1629
Cuts at: 5735 8
Size: 43
Fragments arranged by size:
1629 1425 1387 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2570 3700 1353
Size: 1217 1130 3423
Fragments arranged by size:
3423 1217 1130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2035 2668 4357 4731 4877 387
Size: 1064 138 446 633 1689 374 146 1280
Fragments arranged by size:
1689 1280 1064 633 446 374 146 138
EcoICRI GAG'CTC
Cuts at: 2966 2966
Size: 5770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2286 2479 2534 2852 3086 3833
Size: 393 410 244 193 55 318 234 747
Cuts at: 3833 4430 5217 5485 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 747 597 410 393 318 268
244 234 193 55
EcoRI G'AATT_C
Cuts at: 2085 3079 3457 2085
Size: 994 378 4398
Fragments arranged by size:
4398 994 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2237 2752 2237
Size: 515 5255
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 2070 2488 4917 1678
Size: 392 418 2429 2531
Fragments arranged by size:
2531 2429 418 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2266 2298 4411 4443 4485
Size: 32 135 32 694 32 2113 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 2113 694 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KasI G'GCGC_C
Cuts at: 2066 2066
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 4884 5068 1305
Size: 1155 2424 184 2007
Fragments arranged by size:
2424 2007 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 2627 3041 3728 4087 4246 518
Size: 648 928 533 414 687 359 159 2042
Fragments arranged by size:
2042 928 687 648 533 414 359 159
MspA1I CmG'CkG
Cuts at: 1606 3186 3863 5011 5256 1606
Size: 1580 677 1148 245 2120
Fragments arranged by size:
2120 1580 1148 677 245
NarI GG'CG_CC
Cuts at: 2067 2067
Size: 5770
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 3128 4673 1132
Size: 97 1899 1545 2229
Fragments arranged by size:
2229 1899 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3026 1348
Size: 1678 4092
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PfoI T'CCnGG_A
Cuts at: 2511 2946 2511
Size: 435 5335
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2400 2430 3122 830
Size: 1570 30 692 3478
Fragments arranged by size:
3478 1570 692 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
SacI G_AGCT'C
Cuts at: 2968 2968
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 2570 2570
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2432 4018 5730 2432
Size: 1586 1712 2472
Fragments arranged by size:
2472 1712 1586
SexAI A'CCwGG_T
Cuts at: 2610 2610
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 2187 2338 2396 2426 2705 3118 4253
Size: 219 1142 151 58 30 279 413 1135
Cuts at: 4253 4934 5125 826
Size: 681 191 1471
Fragments arranged by size:
1471 1142 1135 681 413 279 219 191
151 58 30
SfoI GGC'GCC
Cuts at: 2068 2068
Size: 5770
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 2051 3097 1872
Size: 179 1046 4545
Fragments arranged by size:
4545 1046 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2793 2855 3874 4775 5037 820
Size: 197 926 850 62 1019 901 262 1553
Fragments arranged by size:
1553 1019 926 901 850 262 197 62
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 3209 513
Size: 552 939 438 767 3074
Fragments arranged by size:
3074 939 767 552 438
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2204 2409 2430
Size: 274 80 33 51 1443 227 205 21
Cuts at: 2430 2523 3018 4016 5728 96
Size: 93 495 998 1712 138
Fragments arranged by size:
1712 1443 998 495 274 227 205 138
93 80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2917 2949 5730 5762 2917
Size: 32 2781 32 2925
Fragments arranged by size:
2925 2781 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2981 1316
Size: 1665 4105
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 2049 3095 1870
Size: 179 1046 4545
Fragments arranged by size:
4545 1046 179
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2037 3578 276
Size: 53 83 186 936 503 1541 2468
Fragments arranged by size:
2468 1541 936 503 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SfiI SrfI SwaI XcmI