Restriction map for
pFN21AE6343
(Circular) (Six-base) MAPSORT of: pFN21AE6343.seq Check: 2224 from: 1 to: 5638
pFN21AE6343 5638 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2670 2959 2670
Size: 289 5349
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2404 2566 3701 5085 5353 1632
Size: 772 162 1135 1384 268 1917
Fragments arranged by size:
1917 1384 1135 772 268 162
AfeI AGC'GCT
Cuts at: 2104 2104
Size: 5638
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2345 1166
Size: 1179 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2032 2589 2630 3381 4953 2032
Size: 557 41 751 1572 2717
Fragments arranged by size:
2717 1572 751 557 41
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 2875 2963 1870
Size: 73 273 659 88 4545
Fragments arranged by size:
4545 659 273 88 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2372 2647 2670 2959
Size: 325 217 627 279 306 275 23 289
Cuts at: 2959 4414 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 306 289 279 275
217 23
BanII G_rGCy'C
Cuts at: 1391 2901 2957 1391
Size: 1510 56 4072
Fragments arranged by size:
4072 1510 56
BbeI G_GCGC'C
Cuts at: 2070 2070
Size: 5638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2123 2729
Size: 107 122 71 631 605 337 114 606
Cuts at: 2729 4249 136
Size: 1520 1525
Fragments arranged by size:
1525 1520 631 606 605 337 122 114
107 71
BglII A'GATC_T
Cuts at: 2691 5633 2691
Size: 2942 2696
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 2923 1318
Size: 1605 4033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 2235 2646
Size: 416 53 83 399 24 762 56 411
Cuts at: 2646 3369 4327 442
Size: 723 958 1753
Fragments arranged by size:
1753 958 762 723 416 411 399 83
56 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 1239
Size: 3059 2579
Bpu10I CC'TnA_GC
Cuts at: 2895 2895
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2459 3763 4628 4926 2282
Size: 177 1304 865 298 2994
Fragments arranged by size:
2994 1304 865 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2343 2347 3429 493
Size: 711 1139 4 1082 2702
Fragments arranged by size:
2702 1139 1082 711 4
BsaBI GATnn'nnATC
Cuts at: 2696 5632 2696
Size: 2936 2702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2188 3445 3827
Size: 53 83 186 936 534 121 1257 382
Cuts at: 3827 4462 275
Size: 635 1451
Fragments arranged by size:
1451 1257 936 635 534 382 186 121
83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5413
Fragments arranged by size:
5413 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2785 1186
Size: 1599 4039
BseYI C'CCAG_C
Cuts at: 2062 4841 5337 2062
Size: 2779 496 2363
Fragments arranged by size:
2779 2363 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2726 1163
Size: 573 990 4075
Fragments arranged by size:
4075 990 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsiWI C'GTAC_G
Cuts at: 2343 2343
Size: 5638
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
BsmBI CGTCTCn'nnnn_
Cuts at: 2477 2477
Size: 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2901 2957 3699 3784 4855 1391
Size: 399 1111 56 742 85 1071 2174
Fragments arranged by size:
2174 1111 1071 742 399 85 56
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2311 3525 1394
Size: 429 488 1214 3507
Fragments arranged by size:
3507 1214 488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2193 4133 4307 65
Size: 1183 945 1940 174 1396
Fragments arranged by size:
1940 1396 1183 945 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 2055 1925
Size: 130 5508
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 2677 1184
Size: 1493 4145
BstYI r'GATC_y
Cuts at: 1972 2691 2968 3335 3720 3737 5189 5275 5287
Size: 719 277 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 719 385 367 346 277 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 2536 3154 3948 3968 2159
Size: 377 618 794 20 3829
Fragments arranged by size:
3829 794 618 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2389 2654 2389
Size: 265 5373
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603
Size: 54 1425 50 275 18 1255 889 1629
Cuts at: 5603 8
Size: 43
Fragments arranged by size:
1629 1425 1255 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 2576 2708 2768 2789 2795 3568 1353
Size: 1070 153 132 60 21 6 773 3423
Fragments arranged by size:
3423 1070 773 153 132 60 21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2404 2566 3701 4298 5085 5353 1239
Size: 393 772 162 1135 597 787 268 1524
Fragments arranged by size:
1524 1135 787 772 597 393 268 162
EcoO109I rG'GnC_Cy
Cuts at: 2627 2627
Size: 5638
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2070 2106 2761 2828 4785 1678
Size: 392 36 655 67 1957 2531
Fragments arranged by size:
2531 1957 655 392 67 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2404 2982 3228 669
Size: 988 281 466 578 246 3079
Fragments arranged by size:
3079 988 578 466 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KasI G'GCGC_C
Cuts at: 2066 2066
Size: 5638
KpnI G_GTAC'C
Cuts at: 2674 2963 2674
Size: 289 5349
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2345 3596 3955 4114 518
Size: 648 1032 147 1251 359 159 2042
Fragments arranged by size:
2042 1251 1032 648 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2062 2586 3054 3731 4879 5124 1606
Size: 456 524 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 468 456 245
NarI GG'CG_CC
Cuts at: 2067 2067
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2545 2996 4541 1132
Size: 97 1316 451 1545 2229
Fragments arranged by size:
2229 1545 1316 451 97
PasI CC'CwG_GG
Cuts at: 1348 2901 1348
Size: 1553 4085
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 1108
Size: 1281 4357
PfoI T'CCnGG_A
Cuts at: 2766 2766
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2082 2166 2990 830
Size: 1252 84 824 3478
Fragments arranged by size:
3478 1252 824 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SapI GCTCTTCn'nnn_
Cuts at: 2423 2423
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2082 2990 2082
Size: 908 4730
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SexAI A'CCwGG_T
Cuts at: 2385 2673 2385
Size: 288 5350
SfcI C'TryA_G
Cuts at: 826 1045 2078 2162 2986 4121 4802 4993 826
Size: 219 1033 84 824 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 824 681 219 191 84
SfoI GGC'GCC
Cuts at: 2068 2068
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2877 2965 1872
Size: 1005 88 4545
Fragments arranged by size:
4545 1005 88
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2297 2474 3742 4643 4905
Size: 197 926 273 81 177 1268 901 262
Cuts at: 4905 820
Size: 1553
Fragments arranged by size:
1553 1268 926 901 273 262 197 177
81
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 3077 513
Size: 552 939 1073 3074
Fragments arranged by size:
3074 1073 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 2381 2665 3665 3682 3835 4020
Size: 1255 780 21 284 1000 17 153 185
Cuts at: 4020 4465 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1000 780 445 284 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 2661 3884
Size: 274 80 33 51 1443 600 84 1223
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1223 600 274 138 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 4555
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2383 2200
Size: 183 5455
XhoI C'TCGA_G
Cuts at: 1943 2216 1943
Size: 273 5365
XmaI C'CCGG_G
Cuts at: 1870 2875 2963 1870
Size: 1005 88 4545
Fragments arranged by size:
4545 1005 88
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI