Restriction map for
pFN21AE6342
(Circular) (Six-base) MAPSORT of: pFN21AE6342.seq Check: 1605 from: 1 to: 5857
pFN21AE6342 5857 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2911 2911
Size: 5857
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2826 3667 278
Size: 53 83 186 936 1290 841 2468
Fragments arranged by size:
2468 1290 936 841 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 3600 5172 3600
Size: 1572 4285
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 2081 2882 3166 3392 3544 5653 1493
Size: 588 801 284 226 152 2109 1697
Fragments arranged by size:
2109 1697 801 588 284 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3182 1870
Size: 73 67 1172 4545
Fragments arranged by size:
4545 1172 73 67
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2897 3178 4633 618
Size: 325 217 627 1110 281 1455 1842
Fragments arranged by size:
1842 1455 1110 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 3176 1391
Size: 1785 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 2484 2955 1548
Size: 936 471 4450
Fragments arranged by size:
4450 936 471
BfrBI ATG'CAT
Cuts at: 2040 2040
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3044 4468 136
Size: 107 122 71 631 605 1372 1424 1525
Fragments arranged by size:
1525 1424 1372 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2539 2927 5852 2539
Size: 388 2925 2544
Fragments arranged by size:
2925 2544 388
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 3918 5074 1790
Size: 2128 1156 2573
Fragments arranged by size:
2573 2128 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2695 3588 4546
Size: 416 53 83 399 24 1278 893 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1278 958 893 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 2930 4517 1239
Size: 1517 174 1587 2579
Fragments arranged by size:
2579 1587 1517 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 3982 4847 5145 2603
Size: 1379 865 298 3315
Fragments arranged by size:
3315 1379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2706 2823 3664 4046
Size: 53 83 186 936 1173 117 841 382
Cuts at: 4046 4681 275
Size: 635 1451
Fragments arranged by size:
1451 1173 936 841 635 382 186 117
83 53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5632
Fragments arranged by size:
5632 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3095 1186
Size: 1909 3948
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2223 1163
Size: 573 487 4797
Fragments arranged by size:
4797 573 487
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3918 4003 5074 3918
Size: 85 1071 4701
Fragments arranged by size:
4701 1071 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3176 3918 4003 5074 1391
Size: 399 1386 742 85 1071 2174
Fragments arranged by size:
2174 1386 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2911 3212 844
Size: 2067 301 3489
Fragments arranged by size:
3489 2067 301
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2517 96
Size: 2421 3436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 2738 1184
Size: 1554 4303
BstXI CCAn_nnnn'nTGG
Cuts at: 2908 2908
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2539 2927 3187 3554 3939 3956 5408 5494
Size: 567 388 260 367 385 17 1452 86
Cuts at: 5494 5506 5852 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 567 388 385 367 346 260
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2756 3304
Size: 54 1425 50 275 18 388 538 548
Cuts at: 3304 4193 5822 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 548 538 388 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2964 3072 3081 3787 1353
Size: 1611 108 9 706 3423
Fragments arranged by size:
3423 1611 706 108 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2756 2930 3920 4517 5304 5572 1239
Size: 393 1124 174 990 597 787 268 1524
Fragments arranged by size:
1524 1124 990 787 597 393 268 174
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
EcoRV GAT'ATC
Cuts at: 2425 2425
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2057 2765 5004 1678
Size: 379 708 2239 2531
Fragments arranged by size:
2531 2239 708 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 2555 2587 3080
Size: 32 135 32 837 32 114 32 493
Cuts at: 3080 3112 4498 4530 4572 4604 1373
Size: 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 837 493 135 114 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 2574 3456 2574
Size: 882 4975
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2758 10
Size: 54 1425 343 926 3109
Fragments arranged by size:
3109 1425 926 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 3815 4174 4333 518
Size: 648 873 1776 359 159 2042
Fragments arranged by size:
2042 1776 873 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3273 3950 5098 5343 1606
Size: 1667 677 1148 245 2120
Fragments arranged by size:
2120 1667 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 2036 387
Size: 1649 4208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NsiI A_TGCA'T
Cuts at: 2042 2042
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2044 3215 4760 1132
Size: 97 815 1171 1545 2229
Fragments arranged by size:
2229 1545 1171 815 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2158 1613
Size: 545 5312
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2350 3209 830
Size: 1520 859 3478
Fragments arranged by size:
3478 1520 859
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SapI GCTCTTCn'nnn_
Cuts at: 3081 3081
Size: 5857
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2346 3205 4340 5021 5212 826
Size: 219 1301 859 1135 681 191 1471
Fragments arranged by size:
1471 1301 1135 859 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2012 3184 1872
Size: 140 1172 4545
Fragments arranged by size:
4545 1172 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3961 4862 5124 820
Size: 197 926 675 1343 901 262 1553
Fragments arranged by size:
1553 1343 926 901 675 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 2044 3215 1132
Size: 912 1171 3774
Fragments arranged by size:
3774 1171 912
SrfI GCCC'GGGC
Cuts at: 2012 2012
Size: 5857
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2004 3012 3048 3296 513
Size: 552 939 1008 36 248 3074
Fragments arranged by size:
3074 1008 939 552 248 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 3901 4054 4239 4684 325
Size: 1255 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 2517 4103
Size: 274 80 33 51 1443 309 231 1586
Cuts at: 4103 5815 96
Size: 1712 138
Fragments arranged by size:
1712 1586 1443 309 274 231 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 2086 3193 2086
Size: 1107 4750
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2010 3182 1870
Size: 140 1172 4545
Fragments arranged by size:
4545 1172 140
XmnI GAAnn'nnTTC
Cuts at: 2811 3986 2811
Size: 1175 4682
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2824 3665 276
Size: 53 83 186 936 1290 841 2468
Fragments arranged by size:
2468 1290 936 841 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SwaI XcmI