Restriction map for
pFN21AE6341
(Circular) (Six-base) MAPSORT of: pFN21AE6341.seq Check: 904 from: 1 to: 5977
pFN21AE6341 5977 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2116 2116
Size: 5977
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 2874 3298 2874
Size: 424 5553
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4040 5424 5692 1632
Size: 2408 1384 268 1917
Fragments arranged by size:
2408 1917 1384 268
AfeI AGC'GCT
Cuts at: 2698 2698
Size: 5977
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2544 4876 5853 1203
Size: 1341 2332 977 1327
Fragments arranged by size:
2332 1341 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2279 2947 3015 3720 5292 2279
Size: 668 68 705 1572 2964
Fragments arranged by size:
2964 1572 705 668 68
ApaI G_GGCC'C
Cuts at: 2254 2254
Size: 5977
ApaLI G'TGCA_C
Cuts at: 2957 4034 5190 2957
Size: 1077 1156 3744
Fragments arranged by size:
3744 1156 1077
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2254 2420 2892 2930 3302 1870
Size: 73 289 22 166 472 38 372 4545
Fragments arranged by size:
4545 472 372 289 166 73 38 22
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2593 2874 3298 4753
Size: 325 217 627 341 465 281 424 1455
Cuts at: 4753 618
Size: 1842
Fragments arranged by size:
1842 1455 627 465 424 341 325 281
217
BanII G_rGCy'C
Cuts at: 1391 2115 2254 2784 3296 1391
Size: 724 139 530 512 4072
Fragments arranged by size:
4072 724 530 512 139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2842 928
Size: 971 943 4063
Fragments arranged by size:
4063 971 943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2648 2867 4588
Size: 107 122 71 631 605 976 219 1721
Cuts at: 4588 136
Size: 1525
Fragments arranged by size:
1721 1525 976 631 605 219 122 107
71
BglII A'GATC_T
Cuts at: 3268 5972 3268
Size: 2704 3273
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2254 2598 2961 4038 5194 1790
Size: 464 344 363 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 464 363 344
BmgBI CAC'GTC
Cuts at: 1318 2956 1318
Size: 1638 4339
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2995 3708 4666
Size: 416 53 83 399 24 1578 713 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1578 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 2247
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4637 1239
Size: 3398 2579
Bpu10I CC'TnA_GC
Cuts at: 3080 3080
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 4102 4967 5265 2687
Size: 1415 865 298 3399
Fragments arranged by size:
3399 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2545 3768 493
Size: 711 1341 1223 2702
Fragments arranged by size:
2702 1341 1223 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2454 4410 4808 5082 5229 5640 2454
Size: 1956 398 274 147 411 2791
Fragments arranged by size:
2791 1956 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2072 1186
Size: 883 3 5091
Fragments arranged by size:
5091 883 3
BseYI C'CCAG_C
Cuts at: 2121 2439 5180 5676 2121
Size: 318 2741 496 2422
Fragments arranged by size:
2741 2422 496 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 1163
Size: 573 699 4705
Fragments arranged by size:
4705 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2414 2725 3427 4188 5216 1540
Size: 275 186 413 311 702 761 1028 2301
Fragments arranged by size:
2301 1028 761 702 413 311 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2583 2631 2826 2961 4038 4123 5194 2115
Size: 468 48 195 135 1077 85 1071 2898
Fragments arranged by size:
2898 1077 1071 468 195 135 85 48
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
BsmBI CGTCTCn'nnnn_
Cuts at: 2750 2750
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2254 2583 2598 2631 2784 2826
Size: 399 325 139 329 15 33 153 42
Cuts at: 2826 2961 3296 4038 4123 5194 1391
Size: 135 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 329 325 153
139 135 85 42 33 15
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 2277 2955 3332 844
Size: 1272 161 678 377 3489
Fragments arranged by size:
3489 1272 678 377 161
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2603 2642 2909 2961 3000 3129 4621
Size: 110 793 39 267 52 39 129 1492
Cuts at: 4621 4808 1700
Size: 187 2869
Fragments arranged by size:
2869 1492 793 267 187 129 110 52
39 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 3093 1925
Size: 1168 4809
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3015 794
Size: 2221 3756
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 3268 3307 3674 4059 4076 5528 5614 5626
Size: 1296 39 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1296 385 367 346 86 39
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2967 3098 513
Size: 552 1902 131 3392
Fragments arranged by size:
3392 1902 552 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 2658 3493 4287 4307 2658
Size: 835 794 20 4328
Fragments arranged by size:
4328 835 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 2578 2578
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2411 2434
Size: 54 1425 50 275 18 552 29 23
Cuts at: 2434 2640 3424 4313 5942 8
Size: 206 784 889 1629 43
Fragments arranged by size:
1629 1425 889 784 552 275 206 54
50 43 29 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2411 3424 1537
Size: 275 599 1013 4090
Fragments arranged by size:
4090 1013 599 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 2090 3907 1353
Size: 700 37 1817 3423
Fragments arranged by size:
3423 1817 700 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2315 2791 4564 4938 5084 387
Size: 1064 138 726 476 1773 374 146 1280
Fragments arranged by size:
1773 1280 1064 726 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 2113 2113
Size: 5977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4040 4637 5424 5692 1239
Size: 393 2408 597 787 268 1524
Fragments arranged by size:
2408 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2788 2788
Size: 5977
EcoO109I rG'GnC_Cy
Cuts at: 2101 2250 2251 2417 2899 2101
Size: 149 1 166 482 5179
Fragments arranged by size:
5179 482 166 149 1
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 5977
FseI GG_CCGG'CC
Cuts at: 2646 2646
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2279 2335 2700 2710 5124 1678
Size: 601 56 365 10 2414 2531
Fragments arranged by size:
2531 2414 601 365 56 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3119 3151 4618 4650 4692
Size: 32 135 32 1547 32 1467 32 42
Cuts at: 4692 4724 1373
Size: 32 2626
Fragments arranged by size:
2626 1547 1467 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 2878 3302 2878
Size: 424 5553
MfeI C'AATT_G
Cuts at: 2614 3576 2614
Size: 962 5015
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 3935 4294 4453 518
Size: 648 1596 1173 359 159 2042
Fragments arranged by size:
2042 1596 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2366 2439 3100 3393 4070 5218 5463
Size: 693 67 73 661 293 677 1148 245
Cuts at: 5463 1606
Size: 2120
Fragments arranged by size:
2120 1148 693 677 661 293 245 73
67
NaeI GCC'GGC
Cuts at: 2605 2644 3002 2605
Size: 39 358 5580
Fragments arranged by size:
5580 358 39
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5425
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NgoMIV G'CCGG_C
Cuts at: 2603 2642 3000 2603
Size: 39 358 5580
Fragments arranged by size:
5580 358 39
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2044 3335 4880 1132
Size: 97 815 1291 1545 2229
Fragments arranged by size:
2229 1545 1291 815 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 2545 2545
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2417 2101
Size: 316 5661
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 2250 2250
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2074 2100 2304 3011 3329 830
Size: 1244 26 204 707 318 3478
Fragments arranged by size:
3478 1244 707 318 204 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2366 2366
Size: 5977
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 5977
SacI G_AGCT'C
Cuts at: 2115 2115
Size: 5977
SacII CC_GC'GG
Cuts at: 3101 3101
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 2417 2417
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 2100 3329 2100
Size: 1229 4748
ScaI AGT'ACT
Cuts at: 3176 4225 5937 3176
Size: 1049 1712 3216
Fragments arranged by size:
3216 1712 1049
SfcI C'TryA_G
Cuts at: 826 1045 2070 2096 2300 3007 3325 4460 5141
Size: 219 1025 26 204 707 318 1135 681
Cuts at: 5141 5332 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1025 707 681 318 219 204
191 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 2648 2867 2648
Size: 219 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 2422 3304 1872
Size: 550 882 4545
Fragments arranged by size:
4545 882 550
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 4081 4982 5244 820
Size: 197 926 759 1379 901 262 1553
Fragments arranged by size:
1553 1379 926 901 759 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 2076 2492 1517
Size: 559 416 5002
Fragments arranged by size:
5002 559 416
StyI C'CwwG_G
Cuts at: 513 1065 2156 2598 3416 513
Size: 552 1091 442 818 3074
Fragments arranged by size:
3074 1091 818 552 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3174 4223 5935
Size: 274 80 33 51 1443 1197 1049 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1712 1443 1197 1049 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 2993 1316
Size: 1677 4300
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 2420 3302 1870
Size: 550 882 4545
Fragments arranged by size:
4545 882 550
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI
NsiI PacI PfoI PspXI PsrI SapI SexAI SfoI
SrfI SwaI XcmI