Restriction map for
pFN21AE6339
(Circular) (Six-base) MAPSORT of: pFN21AE6339.seq Check: 9664 from: 1 to: 5992
pFN21AE6339 5992 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3027 3335 1937
Size: 1090 308 4594
Fragments arranged by size:
4594 1090 308
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 3087 3218 4055 5439 5707 1632
Size: 707 748 131 837 1384 268 1917
Fragments arranged by size:
1917 1384 837 748 707 268 131
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 2259 4721 2259
Size: 2462 3530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 3735 5307 3735
Size: 1572 4420
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 2758 3301 3527 3679 5788 1493
Size: 1265 543 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2973 3006 2973
Size: 33 5959
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3313 4768 618
Size: 325 217 627 1526 1455 1842
Fragments arranged by size:
1842 1526 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3311 1391
Size: 1920 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5021
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2393 2974 3884 5094 2393
Size: 581 910 1210 3291
Fragments arranged by size:
3291 1210 910 581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4603 136
Size: 107 122 71 631 605 2931 1525
Fragments arranged by size:
2931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 4053 5209 1790
Size: 2263 1156 2573
Fragments arranged by size:
2573 2263 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4681 442
Size: 416 53 83 399 24 2306 958 1753
Fragments arranged by size:
2306 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 4652 1239
Size: 956 2457 2579
Fragments arranged by size:
2579 2457 956
Bpu10I CC'TnA_GC
Cuts at: 2045 2456 2045
Size: 411 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3099 4655 882
Size: 617 1600 1556 2219
Fragments arranged by size:
2219 1600 1556 617
BsaAI yAC'GTr
Cuts at: 493 1204 2935 3783 493
Size: 711 1731 848 2702
Fragments arranged by size:
2702 1731 848 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4181 4816 275
Size: 53 83 186 936 2266 382 635 1451
Fragments arranged by size:
2266 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2376 2406 2670 2700 2717
Size: 30 165 30 973 30 264 30 17
Cuts at: 2717 2747 2835 2865 1178
Size: 30 88 30 4305
Fragments arranged by size:
4305 973 264 165 88 30 30 30
30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5992
BseYI C'CCAG_C
Cuts at: 5195 5691 5195
Size: 496 5496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4053 4138 5209 4053
Size: 85 1071 4836
Fragments arranged by size:
4836 1071 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3311 4053 4138 5209 1391
Size: 399 1521 742 85 1071 2174
Fragments arranged by size:
2174 1521 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 2376 2424 3881 5611 2376
Size: 48 1457 1730 2757
Fragments arranged by size:
2757 1730 1457 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3347 844
Size: 2503 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 1394
Size: 429 2056 3507
Fragments arranged by size:
3507 2056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2349 2403 4487 4661 65
Size: 1183 1101 54 2084 174 1396
Fragments arranged by size:
2084 1396 1183 1101 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 4636 4823 1700
Size: 110 199 2627 187 2869
Fragments arranged by size:
2869 2627 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 2830 2927 96
Size: 2734 97 3161
Fragments arranged by size:
3161 2734 97
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 2968 4796 2968
Size: 1828 4164
BstEII G'GTnAC_C
Cuts at: 1184 2723 1184
Size: 1539 4453
BstYI r'GATC_y
Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987
Size: 1350 367 385 17 1452 86 12 346
Cuts at: 5987 1972
Size: 1977
Fragments arranged by size:
1977 1452 1350 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2794 513
Size: 552 939 790 3711
Fragments arranged by size:
3711 939 790 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2519 487
Size: 683 56 1293 3960
Fragments arranged by size:
3960 1293 683 56
BtsI GCAGTG_nn'
Cuts at: 2820 3508 4302 4322 2820
Size: 688 794 20 4490
Fragments arranged by size:
4490 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 2073 3084 3296 2073
Size: 1011 212 4769
Fragments arranged by size:
4769 1011 212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2154 3439
Size: 54 1425 50 275 18 177 147 1285
Cuts at: 3439 4328 5957 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 275 177 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3922 1353
Size: 2569 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2339 3087 3218 4055 4652 5439
Size: 393 563 144 748 131 837 597 787
Cuts at: 5439 5707 1239
Size: 268 1524
Fragments arranged by size:
1524 837 787 748 597 563 393 268
144 131
EcoNI CCTnn'n_nnAGG
Cuts at: 2769 2769
Size: 5992
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2032 2088 2463 5139 1678
Size: 354 56 375 2676 2531
Fragments arranged by size:
2676 2531 375 354 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2245 2277 2534 2566 3271
Size: 32 135 32 673 32 257 32 705
Cuts at: 3271 3303 4633 4665 4707 4739 1373
Size: 32 1330 32 42 32 2626
Fragments arranged by size:
2626 1330 705 673 257 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2191 2346 2448 3336 3582 669
Size: 988 281 253 155 102 888 246 3079
Fragments arranged by size:
3079 988 888 281 253 246 155 102
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 2191 2448 3582 2191
Size: 257 1134 4601
Fragments arranged by size:
4601 1134 257
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2656 5106 5290 1305
Size: 1351 2450 184 2007
Fragments arranged by size:
2450 2007 1351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3021 3157 3950 4309 4468 518
Size: 648 1855 136 793 359 159 2042
Fragments arranged by size:
2042 1855 793 648 359 159 136
MspA1I CmG'CkG
Cuts at: 1606 2065 2266 2672 3408 4085 5233 5478 1606
Size: 459 201 406 736 677 1148 245 2120
Fragments arranged by size:
2120 1148 736 677 459 406 245 201
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 5992
NcoI C'CATG_G
Cuts at: 513 1065 2004 2794 513
Size: 552 939 790 3711
Fragments arranged by size:
3711 939 790 552
NdeI CA'TA_TG
Cuts at: 387 2442 2628 2975 387
Size: 2055 186 347 3404
Fragments arranged by size:
3404 2055 347 186
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2837 3214 3350 4895 1132
Size: 97 1608 377 136 1545 2229
Fragments arranged by size:
2229 1608 1545 377 136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PfoI T'CCnGG_A
Cuts at: 3142 3142
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 2993 3562 2993
Size: 569 5423
PstI C_TGCA'G
Cuts at: 830 2300 3344 830
Size: 1470 1044 3478
Fragments arranged by size:
3478 1470 1044
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2266 2672 2266
Size: 406 5586
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SfcI C'TryA_G
Cuts at: 826 1045 2296 3340 4475 5156 5347 826
Size: 219 1251 1044 1135 681 191 1471
Fragments arranged by size:
1471 1251 1135 1044 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4096 4997 5259 820
Size: 197 926 2153 901 262 1553
Fragments arranged by size:
2153 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2935 3783 493
Size: 2442 848 2702
Fragments arranged by size:
2702 2442 848
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 2185 1517
Size: 668 5324
StyI C'CwwG_G
Cuts at: 513 1065 2004 2794 3431 513
Size: 552 939 790 637 3074
Fragments arranged by size:
3074 939 790 637 552
SwaI ATTT'AAAT
Cuts at: 3084 3084
Size: 5992
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2769 4019 4036 4189 4374 4819 325
Size: 1255 1189 1250 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1250 1189 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2364 2830 2927
Size: 274 80 33 51 1443 387 466 97
Cuts at: 2927 3169 3221 4238 5950 96
Size: 242 52 1017 1712 138
Fragments arranged by size:
1712 1443 1017 466 387 274 242 138
97 80 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2717 2723 2749 2755 5952 5984 2717
Size: 6 26 6 3197 32 2725
Fragments arranged by size:
3197 2725 32 26 6 6
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI XcmI