Restriction map for
pFN21AE6337
(Circular) (Six-base) MAPSORT of: pFN21AE6337.seq Check: 8437 from: 1 to: 5860
pFN21AE6337 5860 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 3150 2290
Size: 860 5000
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3923 5307 5575 1632
Size: 2291 1384 268 1917
Fragments arranged by size:
2291 1917 1384 268
AfeI AGC'GCT
Cuts at: 2043 2043
Size: 5860
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 2259 1166
Size: 1093 4767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2952 1184
Size: 1768 4092
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 5828
AlwNI CAG_nnn'CTG
Cuts at: 2305 3603 5175 2305
Size: 1298 1572 2990
Fragments arranged by size:
2990 1572 1298
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2735 3169 3395 3547 5656 1493
Size: 1242 434 226 152 2109 1697
Fragments arranged by size:
2109 1697 1242 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 3084 3185 1870
Size: 73 94 1047 101 4545
Fragments arranged by size:
4545 1047 101 94 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2921 2928 3181 4636 618
Size: 325 217 627 1134 7 253 1455 1842
Fragments arranged by size:
1842 1455 1134 627 325 253 217 7
BanII G_rGCy'C
Cuts at: 1391 2147 3179 1391
Size: 756 1032 4072
Fragments arranged by size:
4072 1032 756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BbvCI CC'TCA_GC
Cuts at: 2399 2399
Size: 5860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 2487 2748 3060 1548
Size: 939 261 312 4348
Fragments arranged by size:
4348 939 312 261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4471 136
Size: 107 122 71 631 605 337 2462 1525
Fragments arranged by size:
2462 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2926 3921 5077 1790
Size: 1136 995 1156 2573
Fragments arranged by size:
2573 1156 1136 995
BmgBI CAC'GTC
Cuts at: 1318 2131 1318
Size: 813 5047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 3591 4549
Size: 416 53 83 399 24 811 1363 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1363 958 811 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2381 1056
Size: 1325 4535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2536 3043 2398
Size: 138 507 5215
Fragments arranged by size:
5215 507 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 3013 4520 1239
Size: 1076 698 1507 2579
Fragments arranged by size:
2579 1507 1076 698
Bpu10I CC'TnA_GC
Cuts at: 2187 2399 2187
Size: 212 5648
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3985 4850 5148 3038
Size: 947 865 298 3750
Fragments arranged by size:
3750 947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 4523 882
Size: 617 554 2470 2219
Fragments arranged by size:
2470 2219 617 554
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2163 2193 2856 2886 1178
Size: 30 165 30 760 30 663 30 4152
Fragments arranged by size:
4152 760 663 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 1186
Size: 1114 4746
BseYI C'CCAG_C
Cuts at: 5063 5559 5063
Size: 496 5364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 2798 2916 1163
Size: 573 552 510 118 4107
Fragments arranged by size:
4107 573 552 510 118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 3310 4071 5099 1540
Size: 275 186 93 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 2253 3921 4006 5077 2253
Size: 1668 85 1071 3036
Fragments arranged by size:
3036 1668 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
BsmBI CGTCTCn'nnnn_
Cuts at: 2166 2909 2166
Size: 743 5117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2253 2926 3179 3921 4006 5077
Size: 399 357 106 673 253 742 85 1071
Cuts at: 5077 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 673 399 357 253 106
85
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 2667 3749 5479 2667
Size: 1082 1730 3048
Fragments arranged by size:
3048 1730 1082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 3150 3215 844
Size: 1446 860 65 3489
Fragments arranged by size:
3489 1446 860 65
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 3747 1394
Size: 429 196 1728 3507
Fragments arranged by size:
3507 1728 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 2574 3920 4932 2574
Size: 1346 1012 3502
Fragments arranged by size:
3502 1346 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2776 794
Size: 1982 3878
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 3137 1184
Size: 1953 3907
BstYI r'GATC_y
Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855
Size: 1218 367 385 17 1452 86 12 346
Cuts at: 5855 1972
Size: 1977
Fragments arranged by size:
1977 1452 1218 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2970 513
Size: 552 939 90 876 3403
Fragments arranged by size:
3403 939 876 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 4253
Fragments arranged by size:
4253 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2643 2702 2952 3376 4170 4190 2643
Size: 59 250 424 794 20 4313
Fragments arranged by size:
4313 794 424 250 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2331 2973
Size: 54 1425 50 275 18 261 240 642
Cuts at: 2973 3307 4196 5825 8
Size: 334 889 1629 43
Fragments arranged by size:
1629 1425 889 642 334 275 261 240
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 3307 1537
Size: 275 279 1216 4090
Fragments arranged by size:
4090 1216 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3790 1353
Size: 2437 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 4447 4821 4967 387
Size: 1064 138 452 2406 374 146 1280
Fragments arranged by size:
2406 1280 1064 452 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 3013 3923 4520 5307 5575 1239
Size: 393 683 698 910 597 787 268 1524
Fragments arranged by size:
1524 910 787 698 683 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2261
Size: 5860
EcoO109I rG'GnC_Cy
Cuts at: 2183 2183
Size: 5860
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2623 2431
Size: 192 5668
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2045 5007 1678
Size: 367 2962 2531
Fragments arranged by size:
2962 2531 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 2800 2832 4501
Size: 32 135 32 459 32 737 32 1669
Cuts at: 4501 4533 4575 4607 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1669 737 459 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 3018 4974 5158 1305
Size: 1086 627 1956 184 2007
Fragments arranged by size:
2007 1956 1086 627 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 10
Size: 54 1425 343 501 3537
Fragments arranged by size:
3537 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 2535 2975 3818 4177 4336 518
Size: 648 1093 276 440 843 359 159 2042
Fragments arranged by size:
2042 1093 843 648 440 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2096 2228 2590 2773 3276 3953 5101
Size: 422 68 132 362 183 503 677 1148
Cuts at: 5101 5346 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 503 422 362 245 183
132 68
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4369
Fragments arranged by size:
4369 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 2377 1052
Size: 1325 4535
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2770 3001 3218 4763 1132
Size: 97 1541 231 217 1545 2229
Fragments arranged by size:
2229 1545 1541 231 217 97
PasI CC'CwG_GG
Cuts at: 1348 2063 1348
Size: 715 5145
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2947 1108
Size: 1839 4021
PfoI T'CCnGG_A
Cuts at: 2167 2244 2167
Size: 77 5783
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2697 3147 3212 830
Size: 1867 450 65 3478
Fragments arranged by size:
3478 1867 450 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2773 2773
Size: 5860
SacII CC_GC'GG
Cuts at: 2097 2097
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SexAI A'CCwGG_T
Cuts at: 2481 2481
Size: 5860
SfcI C'TryA_G
Cuts at: 826 1045 2693 2890 3100 3143 3208 4343 5024
Size: 219 1648 197 210 43 65 1135 681
Cuts at: 5024 5215 826
Size: 191 1471
Fragments arranged by size:
1648 1471 1135 681 219 210 197 191
65 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3017 3084 3964 4865 5127 820
Size: 197 926 1074 67 880 901 262 1553
Fragments arranged by size:
1553 1074 926 901 880 262 197 67
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 2770 3218 1132
Size: 1638 448 3774
Fragments arranged by size:
3774 1638 448
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 3299 513
Size: 552 939 1295 3074
Fragments arranged by size:
3074 1295 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2946 3125 3887 3904 4057 4242 4687
Size: 1255 1366 179 762 17 153 185 445
Cuts at: 4687 325
Size: 1498
Fragments arranged by size:
1498 1366 1255 762 445 185 179 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 4106 5818
Size: 274 80 33 51 1443 537 1592 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1592 1443 537 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 2856 2888 5820 5852 2163
Size: 32 661 32 2932 32 2171
Fragments arranged by size:
2932 2171 661 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 2476 3196 2476
Size: 720 5140
XhoI C'TCGA_G
Cuts at: 1943 3084 1943
Size: 1141 4719
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI
SfoI SrfI SwaI XcmI