Restriction map for
pFN21AE6329
(Circular) (Six-base) MAPSORT of: pFN21AE6329.seq Check: 3021 from: 1 to: 5797
pFN21AE6329 5797 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 3860 5244 5512 1632
Size: 710 1518 1384 268 1917
Fragments arranged by size:
1917 1518 1384 710 268
AfeI AGC'GCT
Cuts at: 2865 2865
Size: 5797
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2400 4696 5673 1203
Size: 1197 2296 977 1327
Fragments arranged by size:
2296 1327 1197 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 2139 4526 2139
Size: 2387 3410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2492 2524 2492
Size: 32 5765
AlwNI CAG_nnn'CTG
Cuts at: 2306 2437 3040 3540 5112 2306
Size: 131 603 500 1572 2991
Fragments arranged by size:
2991 1572 603 500 131
ApaI G_GGCC'C
Cuts at: 2045 2045
Size: 5797
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2627 3118 4573 618
Size: 325 217 627 529 311 491 1455 1842
Fragments arranged by size:
1842 1455 627 529 491 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2045 2082 2179 2244 2619 2748 3116 1391
Size: 654 37 97 65 375 129 368 4072
Fragments arranged by size:
4072 654 375 368 129 97 65 37
BbeI G_GCGC'C
Cuts at: 2320 2320
Size: 5797
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2245 928
Size: 971 346 4480
Fragments arranged by size:
4480 971 346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2722 2756 3988 4022 1903
Size: 34 50 34 701 34 1232 34 3678
Fragments arranged by size:
3678 1232 701 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2606 2880 4408
Size: 107 122 71 631 605 934 274 1528
Cuts at: 4408 136
Size: 1525
Fragments arranged by size:
1528 1525 934 631 605 274 122 107
71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 2183 2333 3214 2183
Size: 150 881 4766
Fragments arranged by size:
4766 881 150
Bme1580I G_kGCm'C
Cuts at: 1790 2045 3858 5014 1790
Size: 255 1813 1156 2573
Fragments arranged by size:
2573 1813 1156 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2888 2899 3528
Size: 416 53 83 399 24 1471 11 629
Cuts at: 3528 4486 442
Size: 958 1753
Fragments arranged by size:
1753 1471 958 629 416 399 83 53
24 11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 4457 1239
Size: 857 2361 2579
Fragments arranged by size:
2579 2361 857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2403 3588 493
Size: 711 1199 1185 2702
Fragments arranged by size:
2702 1199 1185 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2317 3604 3986 4621
Size: 53 83 186 936 784 1287 382 635
Cuts at: 4621 275
Size: 1451
Fragments arranged by size:
1451 1287 936 784 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2115 2637 4230 4628 4902 5049 5460 2115
Size: 522 1593 398 274 147 411 2452
Fragments arranged by size:
2452 1593 522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 4453
Fragments arranged by size:
4453 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2540 2939 1186
Size: 856 498 399 4044
Fragments arranged by size:
4044 856 498 399
BseYI C'CCAG_C
Cuts at: 2093 2103 2306 2455 2601 2866 5000 5496 2093
Size: 10 203 149 146 265 2134 496 2394
Fragments arranged by size:
2394 2134 496 265 203 149 146 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2425 1163
Size: 573 689 4535
Fragments arranged by size:
4535 689 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2994 3858 3943 5014 2082
Size: 912 864 85 1071 2865
Fragments arranged by size:
2865 1071 912 864 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
BsmBI CGTCTCn'nnnn_
Cuts at: 2921 2921
Size: 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2082 2179 2244 2619 2748 2994
Size: 399 255 37 97 65 375 129 246
Cuts at: 2994 3116 3858 3943 5014 1391
Size: 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 255 246 129
122 97 85 65 37
BspEI T'CCGG_A
Cuts at: 2115 2637 5460 2115
Size: 522 2823 2452
Fragments arranged by size:
2823 2452 522
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 3152 844
Size: 1832 476 3489
Fragments arranged by size:
3489 1832 476
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 2194 3684 1394
Size: 429 364 7 1490 3507
Fragments arranged by size:
3507 1490 429 364 7
BsrDI GCAATG_nn'
Cuts at: 65 1248 2368 2469 4292 4466 65
Size: 1183 1120 101 1823 174 1396
Fragments arranged by size:
1823 1396 1183 1120 174 101
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2605 2819 4441 4628 1700
Size: 110 297 498 214 1622 187 2869
Fragments arranged by size:
2869 1622 498 297 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2380 2437 3040 794
Size: 1586 57 603 3551
Fragments arranged by size:
3551 1586 603 57
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2208 2778 3127 3494 3879 3896 5348 5434
Size: 236 570 349 367 385 17 1452 86
Cuts at: 5434 5446 5792 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 570 385 367 349 346 236
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2631 2698 2757 3020 513
Size: 552 1566 67 59 263 3290
Fragments arranged by size:
3290 1566 552 263 67 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2959 487
Size: 683 56 1733 3325
Fragments arranged by size:
3325 1733 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2559 3313 4107 4127 2061
Size: 498 754 794 20 3731
Fragments arranged by size:
3731 794 754 498 20
ClaI AT'CG_AT
Cuts at: 1554 2685 1554
Size: 1131 4666
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 2760 3244
Size: 54 1425 50 275 18 843 87 484
Cuts at: 3244 4133 5762 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 843 484 275 87 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3006 3092 3727 1353
Size: 707 946 86 635 3423
Fragments arranged by size:
3423 946 707 635 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2080 2080
Size: 5797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2342 3860 4457 5244 5512 1239
Size: 393 464 246 1518 597 787 268 1524
Fragments arranged by size:
1524 1518 787 597 464 393 268 246
EcoO109I rG'GnC_Cy
Cuts at: 2310 2542 2872 2941 3016 2310
Size: 232 330 69 75 5091
Fragments arranged by size:
5091 330 232 75 69
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2320 2412 2867 2886 3028 4944 1678
Size: 642 92 455 19 142 1916 2531
Fragments arranged by size:
2531 1916 642 455 142 92 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 4438 4470 4512
Size: 32 135 32 553 32 2281 32 42
Cuts at: 4512 4544 1373
Size: 32 2626
Fragments arranged by size:
2626 2281 553 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2316 2316
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 4911 5095 1305
Size: 1173 2433 184 2007
Fragments arranged by size:
2433 2007 1173 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 2762 10
Size: 54 1425 343 843 87 3045
Fragments arranged by size:
3045 1425 843 343 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3755 4114 4273 518
Size: 648 2589 359 159 2042
Fragments arranged by size:
2589 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2329 2455 2655 3040 3213 3890 5038 5283
Size: 723 126 200 385 173 677 1148 245
Cuts at: 5283 1606
Size: 2120
Fragments arranged by size:
2120 1148 723 677 385 245 200 173
126
NaeI GCC'GGC
Cuts at: 2109 2607 2821 2109
Size: 498 214 5085
Fragments arranged by size:
5085 498 214
NarI GG'CG_CC
Cuts at: 2317 2317
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 2757 513
Size: 552 1692 3553
Fragments arranged by size:
3553 1692 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NgoMIV G'CCGG_C
Cuts at: 2107 2605 2819 2107
Size: 498 214 5085
Fragments arranged by size:
5085 498 214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2344 1348
Size: 996 4801
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 2704 2791 1108
Size: 1488 108 87 4114
Fragments arranged by size:
4114 1488 108 87
PfoI T'CCnGG_A
Cuts at: 2511 2511
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PmlI CAC'GTG
Cuts at: 2403 2403
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2492 2524 3866 3898 5379 5411 2492
Size: 32 1342 32 1481 32 2878
Fragments arranged by size:
2878 1481 1342 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2941 3016 2941
Size: 75 5722
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2041 2041
Size: 5797
PstI C_TGCA'G
Cuts at: 830 2442 2614 3036 3149 830
Size: 1612 172 422 113 3478
Fragments arranged by size:
3478 1612 422 172 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2329 2455 3040 2329
Size: 126 585 5086
Fragments arranged by size:
5086 585 126
SacI G_AGCT'C
Cuts at: 2082 2082
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SapI GCTCTTCn'nnn_
Cuts at: 3092 3092
Size: 5797
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2438 2610 3032 3145 4280 4961 5152
Size: 219 1393 172 422 113 1135 681 191
Cuts at: 5152 826
Size: 1471
Fragments arranged by size:
1471 1393 1135 681 422 219 191 172
113
SfoI GGC'GCC
Cuts at: 2318 2318
Size: 5797
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3901 4802 5064 820
Size: 197 926 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2045 2757 2913 2979 3236 513
Size: 552 980 712 156 66 257 3074
Fragments arranged by size:
3074 980 712 552 257 156 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 2844 3824 3841 3994 4179 4624
Size: 1255 1131 133 980 17 153 185 445
Cuts at: 4624 325
Size: 1498
Fragments arranged by size:
1498 1255 1131 980 445 185 153 133
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 2507 3926 2507
Size: 1419 4378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI