Restriction map for
pFN21AE6328
(Circular) (Six-base) MAPSORT of: pFN21AE6328.seq Check: 1760 from: 1 to: 6025
pFN21AE6328 6025 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2144 3368 1937
Size: 207 1224 4594
Fragments arranged by size:
4594 1224 207
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 2942 4152 4525 1563
Size: 1379 1210 373 3063
Fragments arranged by size:
3063 1379 1210 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2741 4088 5472 5740 1632
Size: 1109 1347 1384 268 1917
Fragments arranged by size:
1917 1384 1347 1109 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 2313 2799 4924 5901 1203
Size: 1110 486 2125 977 1327
Fragments arranged by size:
2125 1327 1110 977 486
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 2749
Size: 32 5993
AlwNI CAG_nnn'CTG
Cuts at: 3053 3768 5340 3053
Size: 715 1572 3738
Fragments arranged by size:
3738 1572 715
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 2547 3334 3560 3712 5821 1493
Size: 1054 787 226 152 2109 1697
Fragments arranged by size:
2109 1697 1054 787 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2564 4579 160
Size: 872 1532 2015 1606
Fragments arranged by size:
2015 1606 1532 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 3350 1870
Size: 73 504 903 4545
Fragments arranged by size:
4545 903 504 73
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2948 3346 4801 618
Size: 325 217 627 1161 398 1455 1842
Fragments arranged by size:
1842 1455 1161 627 398 325 217
BanII G_rGCy'C
Cuts at: 1391 3344 1391
Size: 1953 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3917 5127 2814
Size: 1103 1210 3712
Fragments arranged by size:
3712 1210 1103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BfrBI ATG'CAT
Cuts at: 2076 2076
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 3003 4086 5242 1790
Size: 1213 1083 1156 2573
Fragments arranged by size:
2573 1213 1156 1083
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4685 1239
Size: 3446 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2812 4150 5015 5313 2812
Size: 1338 865 298 3524
Fragments arranged by size:
3524 1338 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2307 4688 882
Size: 617 808 2381 2219
Fragments arranged by size:
2381 2219 808 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 1178
Size: 30 165 30 1083 30 4687
Fragments arranged by size:
4687 1083 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6025
BseYI C'CCAG_C
Cuts at: 5228 5724 5228
Size: 496 5529
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2582 1163
Size: 573 846 4606
Fragments arranged by size:
4606 846 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4086 4171 5242 4086
Size: 85 1071 4869
Fragments arranged by size:
4869 1071 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3003 3344 4086 4171 5242 1391
Size: 399 1213 341 742 85 1071 2174
Fragments arranged by size:
2174 1213 1071 742 399 341 85
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 3380 844
Size: 1760 776 3489
Fragments arranged by size:
3489 1760 776
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 1394
Size: 429 2089 3507
Fragments arranged by size:
3507 2089 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4669 4856 1700
Size: 110 2859 187 2869
Fragments arranged by size:
2869 2859 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3053 794
Size: 2259 3766
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020
Size: 1383 367 385 17 1452 86 12 346
Cuts at: 6020 1972
Size: 1977
Fragments arranged by size:
1977 1452 1383 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 3541 4335 4355 3541
Size: 794 20 5211
Fragments arranged by size:
5211 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 2184 2787 3329 2184
Size: 603 542 4880
Fragments arranged by size:
4880 603 542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3472 4361 5990
Size: 54 1425 50 275 18 1642 889 1629
Cuts at: 5990 8
Size: 43
Fragments arranged by size:
1642 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3213 3955 1353
Size: 1860 742 3423
Fragments arranged by size:
3423 1860 742
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 4088 4685 5472 5740 1239
Size: 393 1109 1347 597 787 268 1524
Fragments arranged by size:
1524 1347 1109 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2247 2247
Size: 6025
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2743 2775 4666 4698 4740
Size: 32 135 32 1171 32 1891 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 1891 1171 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2226 3369 3615 669
Size: 988 281 288 1143 246 3079
Fragments arranged by size:
3079 1143 988 288 281 246
HindIII A'AGCT_T
Cuts at: 748 2830 748
Size: 2082 3943
HpaI GTT'AAC
Cuts at: 2226 3615 2226
Size: 1389 4636
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2513 5139 5323 1305
Size: 1208 2626 184 2007
Fragments arranged by size:
2626 2007 1208 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 3983 4342 4501 518
Size: 648 949 1868 359 159 2042
Fragments arranged by size:
2042 1868 949 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3053 3441 4118 5266 5511 1606
Size: 1447 388 677 1148 245 2120
Fragments arranged by size:
2120 1447 1148 677 388 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NsiI A_TGCA'T
Cuts at: 2078 2078
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2120 2317 2407 2500 2803 3163 3383
Size: 97 891 197 90 93 303 360 220
Cuts at: 3383 4928 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 891 360 303 220 197 97
93 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 2313 2799 4924 2313
Size: 486 2125 3414
Fragments arranged by size:
3414 2125 486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2247
Size: 6025
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 2196 3595 2196
Size: 1399 4626
PstI C_TGCA'G
Cuts at: 830 3377 830
Size: 2547 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 3053 3053
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SexAI A'CCwGG_T
Cuts at: 2062 2062
Size: 6025
SfcI C'TryA_G
Cuts at: 826 1045 3373 4508 5189 5380 826
Size: 219 2328 1135 681 191 1471
Fragments arranged by size:
2328 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 2827 4129 5030 5292 820
Size: 197 926 504 380 1302 901 262 1553
Fragments arranged by size:
1553 1302 926 901 504 380 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 3152 152
Size: 3000 3025
SphI G_CATG'C
Cuts at: 1132 3163 3383 1132
Size: 2031 220 3774
Fragments arranged by size:
3774 2031 220
SspI AAT'ATT
Cuts at: 5 52 2173 3949 5
Size: 47 2121 1776 2081
Fragments arranged by size:
2121 2081 1776 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 3464 513
Size: 552 939 1460 3074
Fragments arranged by size:
3074 1460 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 3148 4052 4069 4222 4407 4852
Size: 1255 709 859 904 17 153 185 445
Cuts at: 4852 325
Size: 1498
Fragments arranged by size:
1498 1255 904 859 709 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2605 2637 5985 6017 2605
Size: 32 3348 32 2613
Fragments arranged by size:
3348 2613 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XhoI C'TCGA_G
Cuts at: 1943 2447 1943
Size: 504 5521
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI