Restriction map for
pFN21AE6327
(Circular) (Six-base) MAPSORT of: pFN21AE6327.seq Check: 3542 from: 1 to: 5647
pFN21AE6327 5647 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2582 2582
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2014 2968 2014
Size: 954 4693
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2789 3710 5094 5362 1632
Size: 1157 921 1384 268 1917
Fragments arranged by size:
1917 1384 1157 921 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2150 1166
Size: 984 4663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2168 2432 1184
Size: 984 264 4399
Fragments arranged by size:
4399 984 264
AlwNI CAG_nnn'CTG
Cuts at: 2375 3390 4962 2375
Size: 1015 1572 3060
Fragments arranged by size:
3060 1572 1015
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2006 2039 2006
Size: 33 5614
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2152 2315 2968 4423
Size: 325 217 627 227 138 163 653 1455
Cuts at: 4423 618
Size: 1842
Fragments arranged by size:
1842 1455 653 627 325 227 217 163
138
BanII G_rGCy'C
Cuts at: 1391 2307 2634 2668 2724 2966 1391
Size: 916 327 34 56 242 4072
Fragments arranged by size:
4072 916 327 242 56 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2867 2904 928
Size: 971 968 37 3671
Fragments arranged by size:
3671 971 968 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2792 2826 3838 3872 1903
Size: 34 855 34 1012 34 3678
Fragments arranged by size:
3678 1012 855 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 2604 1548
Size: 1056 4591
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2463 2805 5642 2463
Size: 342 2837 2468
Fragments arranged by size:
2837 2468 342
BlpI GC'TnA_GC
Cuts at: 2814 3064 2814
Size: 250 5397
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 2092 2722 2799 4307 1239
Size: 808 45 630 77 1508 2579
Fragments arranged by size:
2579 1508 808 630 77 45
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2746 3438 493
Size: 711 1542 692 2702
Fragments arranged by size:
2702 1542 711 692
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 4773
BseYI C'CCAG_C
Cuts at: 2306 2405 2435 2818 4850 5346 2306
Size: 99 30 383 2032 496 2607
Fragments arranged by size:
2607 2032 496 383 99 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2616 1163
Size: 573 880 4194
Fragments arranged by size:
4194 880 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2268 3097 3858 4886 1540
Size: 275 186 36 231 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 231 186 36
BsiHKAI G_wGCw'C
Cuts at: 2724 3708 3793 4864 2724
Size: 984 85 1071 3507
Fragments arranged by size:
3507 1071 984 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
BsmBI CGTCTCn'nnnn_
Cuts at: 2081 2081
Size: 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2634 2668 2724 2966 3708 3793
Size: 399 517 327 34 56 242 742 85
Cuts at: 3793 4864 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 327 242 85
56 34
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 2724 3536 5266 2724
Size: 812 1730 3105
Fragments arranged by size:
3105 1730 812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2582 3002 844
Size: 1738 420 3489
Fragments arranged by size:
3489 1738 420
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 2379 4142 4316 65
Size: 1183 1069 62 1763 174 1396
Fragments arranged by size:
1763 1396 1183 1069 174 62
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 4291 4478 1700
Size: 110 345 2136 187 2869
Fragments arranged by size:
2869 2136 345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 2213 2375 794
Size: 1346 73 162 4066
Fragments arranged by size:
4066 1346 162 73
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2158 2233 1184
Size: 974 75 4598
Fragments arranged by size:
4598 974 75
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2463 2805 2977 3344 3729 3746 5198 5284
Size: 491 342 172 367 385 17 1452 86
Cuts at: 5284 5296 5642 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 491 385 367 346 342 172
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2148 2251 2865 513
Size: 552 1083 103 614 3295
Fragments arranged by size:
3295 1083 614 552 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2416 2898 3163 3957 3977 2416
Size: 482 265 794 20 4086
Fragments arranged by size:
4086 794 482 265 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2097 3094
Size: 54 1425 50 275 18 204 63 997
Cuts at: 3094 3983 5612 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 997 889 275 204 63 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 3094 1537
Size: 275 222 1060 4090
Fragments arranged by size:
4090 1060 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 3577 1353
Size: 2224 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2092 2722 2789 2799 3710 4307
Size: 393 415 45 630 67 10 911 597
Cuts at: 4307 5094 5362 1239
Size: 787 268 1524
Fragments arranged by size:
1524 911 787 630 597 415 393 268
67 45 10
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 2144 4153 2144
Size: 2009 3638
FspAI rTGC'GCAy
Cuts at: 2144 2144
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2918 2950 4288 4320 4362
Size: 32 135 32 1346 32 1338 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1346 1338 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2018 2972 2018
Size: 954 4693
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2662 4761 4945 1305
Size: 1357 2099 184 2007
Fragments arranged by size:
2099 2007 1357 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 10
Size: 54 1425 343 267 3558
Fragments arranged by size:
3558 1425 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2673 3605 3964 4123 518
Size: 648 984 523 932 359 159 2042
Fragments arranged by size:
2042 984 932 648 523 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2253 2366 3063 3740 4888 5133 1606
Size: 506 141 113 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 506 245 141 113
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5095
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PfoI T'CCnGG_A
Cuts at: 2577 2577
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2209 2371 2999 830
Size: 1379 162 628 3478
Fragments arranged by size:
3478 1379 628 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2366 2366
Size: 5647
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 5647
SacII CC_GC'GG
Cuts at: 2254 2254
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2197 2205 2367 2449 2995 4130 4811
Size: 219 1152 8 162 82 546 1135 681
Cuts at: 4811 5002 826
Size: 191 1471
Fragments arranged by size:
1471 1152 1135 681 546 219 191 162
82 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2447 1517
Size: 930 4717
StyI C'CwwG_G
Cuts at: 513 1065 2428 3086 513
Size: 552 1363 658 3074
Fragments arranged by size:
3074 1363 658 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 2563 2641 3674 3691 3844 4029
Size: 1255 674 309 78 1033 17 153 185
Cuts at: 4029 4474 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1033 674 445 309 185 153
78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2877 2909 5607 5639 2877
Size: 32 2698 32 2885
Fragments arranged by size:
2885 2698 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2083 2983 2083
Size: 900 4747
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI