Restriction map for
pFN21AE6326
(Circular) (Six-base) MAPSORT of: pFN21AE6326.seq Check: 8063 from: 1 to: 5995
pFN21AE6326 5995 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 3079 4122 4495 1563
Size: 1516 1043 373 3063
Fragments arranged by size:
3063 1516 1043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4058 5442 5710 1632
Size: 2426 1384 268 1917
Fragments arranged by size:
2426 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 2035 3180 4894 5871 1203
Size: 832 1145 1714 977 1327
Fragments arranged by size:
1714 1327 1145 977 832
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 2528 3738 5310 2528
Size: 1210 1572 3213
Fragments arranged by size:
3213 1572 1210
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 2148 2915 3304 3530 3682 5791 1493
Size: 655 767 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 655 389 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3036 3320 1870
Size: 73 1093 284 4545
Fragments arranged by size:
4545 1093 284 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4771 618
Size: 325 217 627 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3314 1391
Size: 1923 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2767 3170 3887 5097 2767
Size: 403 717 1210 3665
Fragments arranged by size:
3665 1210 717 403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 2821 4056 5212 1790
Size: 1031 1235 1156 2573
Fragments arranged by size:
2573 1235 1156 1031
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2271 2550 3044 4655 1239
Size: 912 120 279 494 1611 2579
Fragments arranged by size:
2579 1611 912 494 279 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4120 4985 5283 4120
Size: 865 298 4832
Fragments arranged by size:
4832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4658 882
Size: 617 3159 2219
Fragments arranged by size:
3159 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3802 4184 4819 275
Size: 53 83 186 936 2269 382 635 1451
Fragments arranged by size:
2269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2093 2184 4428 4826 5100 5247 5658 2093
Size: 91 2244 398 274 147 411 2430
Fragments arranged by size:
2430 2244 411 398 274 147 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 1178
Size: 30 165 30 601 30 5139
Fragments arranged by size:
5139 601 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 1186
Size: 1198 4797
BseYI C'CCAG_C
Cuts at: 5198 5694 5198
Size: 496 5499
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4056 4141 5212 4056
Size: 85 1071 4839
Fragments arranged by size:
4839 1071 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2821 3314 4056 4141 5212 1391
Size: 399 1031 493 742 85 1071 2174
Fragments arranged by size:
2174 1071 1031 742 493 399 85
BspEI T'CCGG_A
Cuts at: 2093 2184 5658 2093
Size: 91 3474 2430
Fragments arranged by size:
3474 2430 91
BspHI T'CATG_A
Cuts at: 2665 2856 3884 5614 2665
Size: 191 1028 1730 3046
Fragments arranged by size:
3046 1730 1028 191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3350 844
Size: 2506 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 4490 4664 65
Size: 1183 1275 1967 174 1396
Fragments arranged by size:
1967 1396 1275 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4639 4826 1700
Size: 110 2829 187 2869
Fragments arranged by size:
2869 2829 187 110
BsrGI T'GTAC_A
Cuts at: 96 3154 96
Size: 3058 2937
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 2152 4799 2152
Size: 2647 3348
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 2341 3325 3692 4077 4094 5546 5632 5644
Size: 369 984 367 385 17 1452 86 12
Cuts at: 5644 5990 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 984 385 369 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2446 2686 3163 513
Size: 552 939 442 240 477 3345
Fragments arranged by size:
3345 939 552 477 442 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2095 487
Size: 683 56 869 4387
Fragments arranged by size:
4387 869 683 56
BtsI GCAGTG_nn'
Cuts at: 3093 3511 4305 4325 3093
Size: 418 794 20 4763
Fragments arranged by size:
4763 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2047 3442
Size: 54 1425 50 275 18 177 40 1395
Cuts at: 3442 4331 5960 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1395 889 275 177 54 50
43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 3925 1353
Size: 1473 1099 3423
Fragments arranged by size:
3423 1473 1099
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3079 4582 4956 5102 387
Size: 1064 138 1490 1503 374 146 1280
Fragments arranged by size:
1503 1490 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2271 2550 3044 4058 4655 5442
Size: 393 519 120 279 494 1014 597 787
Cuts at: 5442 5710 1239
Size: 268 1524
Fragments arranged by size:
1524 1014 787 597 519 494 393 279
268 120
EcoO109I rG'GnC_Cy
Cuts at: 2028 2028
Size: 5995
EcoRI G'AATT_C
Cuts at: 2148 2915 3304 3682 2148
Size: 767 389 378 4461
Fragments arranged by size:
4461 767 389 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2315 2976 2315
Size: 661 5334
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 5142 1678
Size: 3464 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2212 2244 2333 2365 3267
Size: 32 135 32 640 32 89 32 902
Cuts at: 3267 3299 4636 4668 4710 4742 1373
Size: 32 1337 32 42 32 2626
Fragments arranged by size:
2626 1337 902 640 135 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2376 2958 3204 748
Size: 1628 582 246 3539
Fragments arranged by size:
3539 1628 582 246
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3195 3594 3195
Size: 399 5596
MluI A'CGCG_T
Cuts at: 2035 5871 2035
Size: 3836 2159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5109 5293 1305
Size: 3804 184 2007
Fragments arranged by size:
3804 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2325 3025 3027 3953 4312 4471 518
Size: 648 1159 700 2 926 359 159 2042
Fragments arranged by size:
2042 1159 926 700 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2068 3064 3411 4088 5236 5481 1606
Size: 462 996 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 996 677 462 347 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2446 2686 3163 513
Size: 552 939 442 240 477 3345
Fragments arranged by size:
3345 939 552 477 442 240
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3184 3353 4898 1132
Size: 97 1955 169 1545 2229
Fragments arranged by size:
2229 1955 1545 169 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 3180 4894 3180
Size: 1714 4281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3236 1108
Size: 2128 3867
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 2028
Size: 5995
PshAI GACnn'nnGTC
Cuts at: 1613 3054 1613
Size: 1441 4554
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2494 3347 830
Size: 1664 853 3478
Fragments arranged by size:
3478 1664 853
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2068 3064 2068
Size: 996 4999
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SanDI GG'GwC_CC
Cuts at: 2028 2028
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2490 2984 3343 4478 5159 5350 826
Size: 219 1445 494 359 1135 681 191 1471
Fragments arranged by size:
1471 1445 1135 681 494 359 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4099 5000 5262 820
Size: 197 926 2156 901 262 1553
Fragments arranged by size:
2156 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 2311 3113 3919 5
Size: 47 2259 802 806 2081
Fragments arranged by size:
2259 2081 806 802 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5995
StyI C'CwwG_G
Cuts at: 513 1065 2004 2446 2686 3163 3434 513
Size: 552 939 442 240 477 271 3074
Fragments arranged by size:
3074 939 552 477 442 271 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4022 4039 4192 4377 4822 325
Size: 1255 2442 17 153 185 445 1498
Fragments arranged by size:
2442 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3154 4241
Size: 274 80 33 51 1443 369 808 1087
Cuts at: 4241 5953 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1087 808 369 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2592 2624 5955 5987 2004
Size: 32 556 32 3331 32 2012
Fragments arranged by size:
3331 2012 556 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 2110 3331 2110
Size: 1221 4774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3100 3100
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 2161 2204 2220 4124 2161
Size: 43 16 1904 4032
Fragments arranged by size:
4032 1904 43 16
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI