Restriction map for
pFN21AE6325
(Circular) (Six-base) MAPSORT of: pFN21AE6325.seq Check: 55 from: 1 to: 6010
pFN21AE6325 6010 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 2412 3038 4073 5457 5725 1632
Size: 683 97 626 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 683 626 268 97
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 4909 5886 1203
Size: 3706 977 1327
Fragments arranged by size:
3706 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6010
AlwNI CAG_nnn'CTG
Cuts at: 2773 3753 5325 2773
Size: 980 1572 3458
Fragments arranged by size:
3458 1572 980
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2997 3092 3319 3545 3697 5806 1493
Size: 1504 95 227 226 152 2109 1697
Fragments arranged by size:
2109 1697 1504 227 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 3335 1870
Size: 73 1392 4545
Fragments arranged by size:
4545 1392 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3229 3262 3229
Size: 33 5977
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3126 3331 4786 618
Size: 325 217 627 1339 205 1455 1842
Fragments arranged by size:
1842 1455 1339 627 325 217 205
BanII G_rGCy'C
Cuts at: 1391 2433 3329 1391
Size: 1042 896 4072
Fragments arranged by size:
4072 1042 896
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 928
Size: 971 560 4479
Fragments arranged by size:
4479 971 560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4201 4235 1903
Size: 34 2264 34 3678
Fragments arranged by size:
3678 2264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 2571 1548
Size: 1023 4987
BfrBI ATG'CAT
Cuts at: 2705 2705
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4621 136
Size: 107 122 71 631 605 2949 1525
Fragments arranged by size:
2949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2051 6005 2051
Size: 3954 2056
BlpI GC'TnA_GC
Cuts at: 2432 3427 2432
Size: 995 5015
Bme1580I G_kGCm'C
Cuts at: 1790 4071 5227 1790
Size: 2281 1156 2573
Fragments arranged by size:
2573 2281 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 2712 3162
Size: 416 53 83 399 24 803 492 450
Cuts at: 3162 3741 4699 442
Size: 579 958 1753
Fragments arranged by size:
1753 958 803 579 492 450 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2795 4670 1239
Size: 1556 1875 2579
Fragments arranged by size:
2579 1875 1556
Bpu10I CC'TnA_GC
Cuts at: 2783 2783
Size: 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 4135 5000 5298 2195
Size: 1940 865 298 2907
Fragments arranged by size:
2907 1940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3817 4199 4834 275
Size: 53 83 186 936 2284 382 635 1451
Fragments arranged by size:
2284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 4443 4841 5115 5262 5673 2499
Size: 1944 398 274 147 411 2836
Fragments arranged by size:
2836 1944 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3279 3309 1178
Size: 30 165 30 1876 30 3879
Fragments arranged by size:
3879 1876 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6010
BseYI C'CCAG_C
Cuts at: 2437 5213 5709 2437
Size: 2776 496 2738
Fragments arranged by size:
2776 2738 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5437
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2433 4071 4156 5227 2433
Size: 1638 85 1071 3216
Fragments arranged by size:
3216 1638 1071 85
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2433 3329 4071 4156 5227 1391
Size: 399 643 896 742 85 1071 2174
Fragments arranged by size:
2174 1071 896 742 643 399 85
BspEI T'CCGG_A
Cuts at: 2499 5673 2499
Size: 3174 2836
BspHI T'CATG_A
Cuts at: 2536 3899 5629 2536
Size: 1363 1730 2917
Fragments arranged by size:
2917 1730 1363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3365 844
Size: 2521 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3047 3181 4505 4679 65
Size: 1183 1799 134 1324 174 1396
Fragments arranged by size:
1799 1396 1324 1183 174 134
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6010
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2051 2626 3340 3707 4092 4109 5561 5647
Size: 79 575 714 367 385 17 1452 86
Cuts at: 5647 5659 6005 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 714 575 385 367 346 86
79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 3526 4320 4340 3526
Size: 794 20 5196
Fragments arranged by size:
5196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3084 3457 4346
Size: 54 1425 50 275 18 1254 373 889
Cuts at: 4346 5975 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1254 889 373 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2106 3940 1353
Size: 720 33 1834 3423
Fragments arranged by size:
3423 1834 720 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2398 4597 4971 5117 387
Size: 1064 138 809 2199 374 146 1280
Fragments arranged by size:
2199 1280 1064 809 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 2431
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2412 2795 3038 4073 4670 5457
Size: 393 683 97 383 243 1035 597 787
Cuts at: 5457 5725 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 787 683 597 393 383 268
243 97
EcoNI CCTnn'n_nnAGG
Cuts at: 3086 3086
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 2779 2779
Size: 6010
EcoRI G'AATT_C
Cuts at: 3092 3319 3697 3092
Size: 227 378 5405
Fragments arranged by size:
5405 378 227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2135 2135
Size: 6010
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 5157 1678
Size: 3479 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 3218 3250 4651
Size: 32 135 32 1265 32 349 32 1401
Cuts at: 4651 4683 4725 4757 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 1265 349 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3354 3600 669
Size: 988 281 1368 48 246 3079
Fragments arranged by size:
3079 1368 988 281 246 48
HindIII A'AGCT_T
Cuts at: 748 2117 2297 748
Size: 1369 180 4461
Fragments arranged by size:
4461 1369 180
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2081 5124 5308 1305
Size: 776 3043 184 2007
Fragments arranged by size:
3043 2007 776 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3086 10
Size: 54 1425 343 1254 2934
Fragments arranged by size:
2934 1425 1254 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3012 3968 4327 4486 518
Size: 648 1846 956 359 159 2042
Fragments arranged by size:
2042 1846 956 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2390 2809 3426 4103 5251 5496 1606
Size: 784 419 617 677 1148 245 2120
Fragments arranged by size:
2120 1148 784 677 617 419 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NsiI A_TGCA'T
Cuts at: 2707 2707
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 3368 4913 1132
Size: 97 2139 1545 2229
Fragments arranged by size:
2229 2139 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 4909 4909
Size: 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2773 1108
Size: 1665 4345
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 2779
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PstI C_TGCA'G
Cuts at: 830 3362 830
Size: 2532 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2390 2809 2390
Size: 419 5591
SacI G_AGCT'C
Cuts at: 2433 2433
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SfcI C'TryA_G
Cuts at: 826 1045 3358 4493 5174 5365 826
Size: 219 2313 1135 681 191 1471
Fragments arranged by size:
2313 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 4114 5015 5277 820
Size: 197 926 231 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 2828 3934 5
Size: 47 2776 1106 2081
Fragments arranged by size:
2776 2081 1106 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2004 3087 3449 513
Size: 552 939 1083 362 3074
Fragments arranged by size:
3074 1083 939 552 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3114 4037 4054 4207 4392 4837 325
Size: 1255 1534 923 17 153 185 445 1498
Fragments arranged by size:
1534 1498 1255 923 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4256 5968 96
Size: 274 80 33 51 1443 2279 1712 138
Fragments arranged by size:
2279 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 3279 3311 5970 6002 2212
Size: 32 1035 32 2659 32 2220
Fragments arranged by size:
2659 2220 1035 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 2743 3346 2743
Size: 603 5407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2376 2376
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 2495 4139 2268
Size: 227 1644 4139
Fragments arranged by size:
4139 1644 227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI