Restriction map for
pFN21AE6324
(Circular) (Six-base) MAPSORT of: pFN21AE6324.seq Check: 6593 from: 1 to: 6019
pFN21AE6324 6019 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2916 3159 2916
Size: 243 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3829 278
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3362 1937
Size: 1425 4594
Acc65I G'GTAC_C
Cuts at: 2255 2449 3340 2255
Size: 194 891 4934
Fragments arranged by size:
4934 891 194
AclI AA'CG_TT
Cuts at: 1563 4146 4519 1563
Size: 2583 373 3063
Fragments arranged by size:
3063 2583 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 3043 4082 5466 5734 1632
Size: 1079 332 1039 1384 268 1917
Fragments arranged by size:
1917 1384 1079 1039 332 268
AfeI AGC'GCT
Cuts at: 2582 2582
Size: 6019
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5822
AflIII A'CryG_T
Cuts at: 1203 4918 5895 1203
Size: 3715 977 1327
Fragments arranged by size:
3715 1327 977
AgeI A'CCGG_T
Cuts at: 4850 4850
Size: 6019
AhdI GACnn_n'nnGTC
Cuts at: 4748 4748
Size: 6019
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6019
AlwNI CAG_nnn'CTG
Cuts at: 2621 2809 3762 5334 2621
Size: 188 953 1572 3306
Fragments arranged by size:
3306 1572 953 188
ApaI G_GGCC'C
Cuts at: 2698 3275 2698
Size: 577 5442
ApaLI G'TGCA_C
Cuts at: 4076 5232 4076
Size: 1156 4863
ApoI r'AATT_y
Cuts at: 1493 3328 3554 3706 5815 1493
Size: 1835 226 152 2109 1697
Fragments arranged by size:
2109 1835 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4573 160
Size: 872 3541 1606
Fragments arranged by size:
3541 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
AvaI C'yCGr_G
Cuts at: 1870 1943 3344 1870
Size: 73 1401 4545
Fragments arranged by size:
4545 1401 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2285 2318 2285
Size: 33 5986
BamHI G'GATC_C
Cuts at: 2968 3349 3716 2968
Size: 381 367 5271
Fragments arranged by size:
5271 381 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 2255 2449 3340 4795
Size: 325 217 627 313 155 194 891 1455
Cuts at: 4795 618
Size: 1842
Fragments arranged by size:
1842 1455 891 627 325 313 217 194
155
BanII G_rGCy'C
Cuts at: 1391 2698 3177 3186 3275 3338 1391
Size: 1307 479 9 89 63 4072
Fragments arranged by size:
4072 1307 479 89 63 9
BbeI G_GCGC'C
Cuts at: 2104 2104
Size: 6019
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2413 928
Size: 971 514 4534
Fragments arranged by size:
4534 971 514
BbvCI CC'TCA_GC
Cuts at: 2963 2963
Size: 6019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4210 4244 1903
Size: 34 2273 34 3678
Fragments arranged by size:
3678 2273 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3911 5121 3911
Size: 1210 4809
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2228 4630
Size: 107 122 71 631 605 337 219 2402
Cuts at: 4630 136
Size: 1525
Fragments arranged by size:
2402 1525 631 605 337 219 122 107
71
BglII A'GATC_T
Cuts at: 6014 6014
Size: 6019
BlpI GC'TnA_GC
Cuts at: 3436 3436
Size: 6019
Bme1580I G_kGCm'C
Cuts at: 1790 2698 3275 4080 5236 1790
Size: 908 577 805 1156 2573
Fragments arranged by size:
2573 1156 908 805 577
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2525 3750 4708
Size: 416 53 83 399 24 1108 1225 958
Cuts at: 4708 442
Size: 1753
Fragments arranged by size:
1753 1225 1108 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2777 4679 1239
Size: 1538 1902 2579
Fragments arranged by size:
2579 1902 1538
Bpu10I CC'TnA_GC
Cuts at: 2963 3155 2963
Size: 192 5827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4144 5009 5307 4144
Size: 865 298 4856
Fragments arranged by size:
4856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4682 882
Size: 617 3183 2219
Fragments arranged by size:
3183 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3810 493
Size: 711 2606 2702
Fragments arranged by size:
2702 2606 711
BsaBI GATnn'nnATC
Cuts at: 6013 6013
Size: 6019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2101 2244 3826 4208
Size: 53 83 186 936 568 143 1582 382
Cuts at: 4208 4843 275
Size: 635 1451
Fragments arranged by size:
1582 1451 936 635 568 382 186 143
83 53
BsaWI w'CCGG_w
Cuts at: 2036 2288 4452 4850 5124 5271 5682 2036
Size: 252 2164 398 274 147 411 2373
Fragments arranged by size:
2373 2164 411 398 274 252 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5794
Fragments arranged by size:
5794 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 1186
Size: 1474 4545
BseYI C'CCAG_C
Cuts at: 2012 2305 2577 2583 2589 2707 2737 2765 2994
Size: 293 272 6 6 118 30 28 229
Cuts at: 2994 3169 3268 5222 5718 2012
Size: 175 99 1954 496 2313
Fragments arranged by size:
2313 1954 496 293 272 229 175 118
99 30 28 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3117 3215 1163
Size: 573 1381 98 3967
Fragments arranged by size:
3967 1381 573 98
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 3469 4230 5258 1540
Size: 275 186 31 1437 761 1028 2301
Fragments arranged by size:
2301 1437 1028 761 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 2397 3186 3296 4080 4165 5236 2397
Size: 789 110 784 85 1071 3180
Fragments arranged by size:
3180 1071 789 784 110 85
BsmI GAATG_Cn'
Cuts at: 3530 3623 3530
Size: 93 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2698 3177 3186 3275 3296 3338
Size: 399 607 301 479 9 89 21 42
Cuts at: 3338 4080 4165 5236 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 479 399 301 89
85 42 21 9
BspEI T'CCGG_A
Cuts at: 5682 5682
Size: 6019
BspHI T'CATG_A
Cuts at: 3908 5638 3908
Size: 1730 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2450 2916 3159 3374 844
Size: 1606 466 243 215 3489
Fragments arranged by size:
3489 1606 466 243 215
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 2784 3066 3906 1394
Size: 429 275 686 282 840 3507
Fragments arranged by size:
3507 840 686 429 282 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 2078 4514 4688 65
Size: 1183 830 2436 174 1396
Fragments arranged by size:
2436 1396 1183 830 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 4663 4850 1700
Size: 110 221 2632 187 2869
Fragments arranged by size:
2869 2632 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 2319 96
Size: 2223 3796
BssHII G'CGCG_C
Cuts at: 1925 2083 1925
Size: 158 5861
BssSI C'ACGA_G
Cuts at: 4079 5091 4079
Size: 1012 5007
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 4736
BstBI TT'CG_AA
Cuts at: 4823 4823
Size: 6019
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6019
BstYI r'GATC_y
Cuts at: 1972 2968 3349 3716 4101 4118 5570 5656 5668
Size: 996 381 367 385 17 1452 86 12
Cuts at: 5668 6014 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 996 385 381 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2538 2689 513
Size: 552 939 39 495 151 3843
Fragments arranged by size:
3843 939 552 495 151 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5280
Fragments arranged by size:
5280 683 56
BtsI GCAGTG_nn'
Cuts at: 3104 3535 4329 4349 3104
Size: 431 794 20 4774
Fragments arranged by size:
4774 794 431 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5984
DraI TTT'AAA
Cuts at: 3323 3323
Size: 6019
DraIII CAC_nnn'GTG
Cuts at: 2194 2194
Size: 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5026 809
Size: 507 3710 1802
Fragments arranged by size:
3710 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2139 2229
Size: 54 1425 50 275 18 199 110 90
Cuts at: 2229 2535 2797 3466 4355 5984 8
Size: 306 262 669 889 1629 43
Fragments arranged by size:
1629 1425 889 669 306 275 262 199
110 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2029 3466 1537
Size: 275 217 1437 4090
Fragments arranged by size:
4090 1437 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2525 2555 2648 2858 3035 3949 1353
Size: 1172 30 93 210 177 914 3423
Fragments arranged by size:
3423 1172 914 210 177 93 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 4606 4980 5126 387
Size: 1064 138 585 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 585 374 146 138
EcoICRI GAG'CTC
Cuts at: 3184 3184
Size: 6019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2711 2777 3043 4082 4679 5466 5734
Size: 393 1079 66 266 1039 597 787 268
Cuts at: 5734 1239
Size: 1524
Fragments arranged by size:
1524 1079 1039 787 597 393 268 266
66
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2843 2977 2159
Size: 684 134 5201
Fragments arranged by size:
5201 684 134
EcoO109I rG'GnC_Cy
Cuts at: 2694 2695 2694
Size: 1 6018
EcoRI G'AATT_C
Cuts at: 3328 3706 3328
Size: 378 5641
FspI TGC'GCA
Cuts at: 4525 4525
Size: 6019
HaeII r_GCGC'y
Cuts at: 1678 2104 2584 2836 2852 5166 1678
Size: 426 480 252 16 2314 2531
Fragments arranged by size:
2531 2314 480 426 252 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4660 4692 4734 4766 1373
Size: 32 135 32 3088 32 42 32 2626
Fragments arranged by size:
3088 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2886 3363 3609 669
Size: 988 281 948 477 246 3079
Fragments arranged by size:
3079 988 948 477 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6019
HpaI GTT'AAC
Cuts at: 3609 3609
Size: 6019
KasI G'GCGC_C
Cuts at: 2100 2100
Size: 6019
KpnI G_GTAC'C
Cuts at: 2259 2453 3344 2259
Size: 194 891 4934
Fragments arranged by size:
4934 891 194
MfeI C'AATT_G
Cuts at: 3618 3618
Size: 6019
MluI A'CGCG_T
Cuts at: 5895 5895
Size: 6019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3063 5133 5317 1305
Size: 1758 2070 184 2007
Fragments arranged by size:
2070 2007 1758 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 10
Size: 54 1425 343 705 3492
Fragments arranged by size:
3492 1425 705 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2369 2507 3977 4336 4495 518
Size: 648 1161 42 138 1470 359 159 2042
Fragments arranged by size:
2042 1470 1161 648 359 159 138 42
MspA1I CmG'CkG
Cuts at: 1606 2045 2309 2769 3435 4112 5260 5505 1606
Size: 439 264 460 666 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 460 439 264 245
NarI GG'CG_CC
Cuts at: 2101 2101
Size: 6019
NcoI C'CATG_G
Cuts at: 513 1065 2004 2538 513
Size: 552 939 534 3994
Fragments arranged by size:
3994 939 552 534
NdeI CA'TA_TG
Cuts at: 387 3236 387
Size: 2849 3170
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6019
NotI GC'GGCC_GC
Cuts at: 3466 3466
Size: 6019
NspI r_CATG'y
Cuts at: 1132 1229 3377 4922 1132
Size: 97 2148 1545 2229
Fragments arranged by size:
2229 2148 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2651 1348
Size: 1303 4716
PciI A'CATG_T
Cuts at: 4918 4918
Size: 6019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6019
PfoI T'CCnGG_A
Cuts at: 3188 3188
Size: 6019
PmeI GTTT'AAAC
Cuts at: 3323 3323
Size: 6019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 5601 5633 4088
Size: 32 1481 32 4474
Fragments arranged by size:
4474 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6019
PsiI TTA'TAA
Cuts at: 3589 3589
Size: 6019
PspOMI G'GGCC_C
Cuts at: 2694 3271 2694
Size: 577 5442
PstI C_TGCA'G
Cuts at: 830 2082 2280 2927 3218 3261 3371 830
Size: 1252 198 647 291 43 110 3478
Fragments arranged by size:
3478 1252 647 291 198 110 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6019
PvuII CAG'CTG
Cuts at: 2309 2309
Size: 6019
RsrII CG'GwC_CG
Cuts at: 2034 2034
Size: 6019
SacI G_AGCT'C
Cuts at: 3186 3186
Size: 6019
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 6019
SalI G'TCGA_C
Cuts at: 1936 3361 1936
Size: 1425 4594
SapI GCTCTTCn'nnn_
Cuts at: 2525 2555 2648 2858 2525
Size: 30 93 210 5686
Fragments arranged by size:
5686 210 93 30
SbfI CC_TGCA'GG
Cuts at: 3371 3371
Size: 6019
ScaI AGT'ACT
Cuts at: 4267 5979 4267
Size: 1712 4307
SfcI C'TryA_G
Cuts at: 826 1045 2078 2276 2923 3214 3257 3367 4502
Size: 219 1033 198 647 291 43 110 1135
Cuts at: 4502 5183 5374 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1033 681 647 291 219 198
191 110 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 2228 2228
Size: 6019
SfoI GGC'GCC
Cuts at: 2102 2102
Size: 6019
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6019
SmaI CCC'GGG
Cuts at: 1872 3346 1872
Size: 1474 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4123 5024 5286 820
Size: 197 926 2180 901 262 1553
Fragments arranged by size:
2180 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3810 493
Size: 3317 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6019
SphI G_CATG'C
Cuts at: 1132 3377 1132
Size: 2245 3774
SspI AAT'ATT
Cuts at: 5 52 3943 5
Size: 47 3891 2081
Fragments arranged by size:
3891 2081 47
StuI AGG'CCT
Cuts at: 1517 2222 1517
Size: 705 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2538 2794 3458 513
Size: 552 939 534 256 664 3074
Fragments arranged by size:
3074 939 664 552 534 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3022 3311 4046 4063 4216 4401 4846
Size: 1255 1442 289 735 17 153 185 445
Cuts at: 4846 325
Size: 1498
Fragments arranged by size:
1498 1442 1255 735 445 289 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 2337 4265
Size: 274 80 33 51 1443 342 18 1928
Cuts at: 4265 5977 96
Size: 1712 138
Fragments arranged by size:
1928 1712 1443 342 274 138 80 51
33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 5979 6011 2283
Size: 32 3664 32 2291
Fragments arranged by size:
3664 2291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6019
XbaI T'CTAG_A
Cuts at: 3355 3355
Size: 6019
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6019
XmaI C'CCGG_G
Cuts at: 1870 3344 1870
Size: 1474 4545
XmnI GAAnn'nnTTC
Cuts at: 4148 4148
Size: 6019
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3827 276
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI SanDI
SexAI SrfI SwaI XcmI