Restriction map for
pFN21AE6323
(Circular) (Six-base) MAPSORT of: pFN21AE6323.seq Check: 2585 from: 1 to: 6025
pFN21AE6323 6025 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2243 2513 3231 3248 4088 5472 5740
Size: 492 119 270 718 17 840 1384 268
Cuts at: 5740 1632
Size: 1917
Fragments arranged by size:
1917 1384 840 718 492 270 268 119
17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 3209 3768 5340 3209
Size: 559 1572 3894
Fragments arranged by size:
3894 1572 559
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 2154 2725 3242 3334 3560 3712 5821 1493
Size: 661 571 517 92 226 152 2109 1697
Fragments arranged by size:
2109 1697 661 571 517 226 152 92
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 3350 1870
Size: 73 1407 4545
Fragments arranged by size:
4545 1407 73
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3346 4801 618
Size: 325 217 627 1559 1455 1842
Fragments arranged by size:
1842 1559 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3344 1391
Size: 1953 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 2988 928
Size: 971 602 487 3965
Fragments arranged by size:
3965 971 602 487
BbvCI CC'TCA_GC
Cuts at: 2574 2574
Size: 6025
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2500 2534 4216 4250 1903
Size: 34 563 34 1682 34 3678
Fragments arranged by size:
3678 1682 563 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BfrBI ATG'CAT
Cuts at: 2511 2511
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 4086 5242 1790
Size: 2296 1156 2573
Fragments arranged by size:
2573 2296 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3134 4685 1239
Size: 1895 1551 2579
Fragments arranged by size:
2579 1895 1551
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2866 4150 5015 5313 2573
Size: 293 1284 865 298 3285
Fragments arranged by size:
3285 1284 865 298 293
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3242 4688 882
Size: 617 1743 1446 2219
Fragments arranged by size:
2219 1743 1446 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2893 4458 4856 5130 5277 5688 2893
Size: 1565 398 274 147 411 3230
Fragments arranged by size:
3230 1565 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 1178
Size: 30 165 30 900 30 4870
Fragments arranged by size:
4870 900 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 1186
Size: 1270 4755
BseYI C'CCAG_C
Cuts at: 5228 5724 5228
Size: 496 5529
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3149 1163
Size: 573 1413 4039
Fragments arranged by size:
4039 1413 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2660 4086 4171 5242 2660
Size: 1426 85 1071 3443
Fragments arranged by size:
3443 1426 1071 85
BsmI GAATG_Cn'
Cuts at: 2513 2903 2991 3005 3536 3629 2513
Size: 390 88 14 531 93 4909
Fragments arranged by size:
4909 531 390 93 88 14
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2660 3344 4086 4171 5242 1391
Size: 399 870 684 742 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 684 399 85
BspEI T'CCGG_A
Cuts at: 2893 5688 2893
Size: 2795 3230
BspHI T'CATG_A
Cuts at: 2848 3914 5644 2848
Size: 1066 1730 3229
Fragments arranged by size:
3229 1730 1066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 3912 1394
Size: 429 220 1869 3507
Fragments arranged by size:
3507 1869 429 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4669 4856 1700
Size: 110 2859 187 2869
Fragments arranged by size:
2869 2859 187 110
BsrGI T'GTAC_A
Cuts at: 96 2019 96
Size: 1923 4102
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6025
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 2236
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2672 3082 3355 3722 4107 4124 5576 5662
Size: 700 410 273 367 385 17 1452 86
Cuts at: 5662 5674 6020 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 700 410 385 367 346 273
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 3541 4335 4355 3541
Size: 794 20 5211
Fragments arranged by size:
5211 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 2679 3329 2679
Size: 650 5375
DraIII CAC_nnn'GTG
Cuts at: 3172 3172
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 3472 4361
Size: 54 1425 50 275 18 750 892 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 892 889 750 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3129 3224 3955 1353
Size: 1776 95 731 3423
Fragments arranged by size:
3423 1776 731 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3194 4612 4986 5132 387
Size: 1064 138 1605 1418 374 146 1280
Fragments arranged by size:
1605 1418 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2243 2513 3134 3231 3248 4088
Size: 393 492 119 270 621 97 17 840
Cuts at: 4088 4685 5472 5740 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 840 787 621 597 492 393 270
268 119 97 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2906 2906
Size: 6025
EcoO109I rG'GnC_Cy
Cuts at: 2301 2301
Size: 6025
EcoRI G'AATT_C
Cuts at: 2725 3334 3712 2725
Size: 609 378 5038
Fragments arranged by size:
5038 609 378
EcoRV GAT'ATC
Cuts at: 3289 3289
Size: 6025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 6025
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2593 2625 4666
Size: 32 135 32 448 32 541 32 2041
Cuts at: 4666 4698 4740 4772 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2041 541 448 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2956 748
Size: 2208 3817
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3070 3335 5139 5323 1305
Size: 1765 265 1804 184 2007
Fragments arranged by size:
2007 1804 1765 265 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 3453
Fragments arranged by size:
3453 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 3983 4342 4501 518
Size: 648 972 1845 359 159 2042
Fragments arranged by size:
2042 1845 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2578 3441 4118 5266 5511 1606
Size: 645 327 863 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 645 327 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NsiI A_TGCA'T
Cuts at: 2513 2513
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2515 3383 4928 1132
Size: 97 1286 868 1545 2229
Fragments arranged by size:
2229 1545 1286 868 97
PasI CC'CwG_GG
Cuts at: 1348 2201 2266 1348
Size: 853 65 5107
Fragments arranged by size:
5107 853 65
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2352 2384 4094 4126 5607 5639 2352
Size: 32 1710 32 1481 32 2738
Fragments arranged by size:
2738 1710 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2301 2301
Size: 6025
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2749 2762 3377 830
Size: 1919 13 615 3478
Fragments arranged by size:
3478 1919 615 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2578 2578
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2169 2745 2758 3057 3373 4508 5189
Size: 219 1124 576 13 299 316 1135 681
Cuts at: 5189 5380 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1124 681 576 316 299 219
191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 2881 4129 5030 5292 820
Size: 197 926 645 293 1248 901 262 1553
Fragments arranged by size:
1553 1248 926 901 645 293 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 2515 3383 1132
Size: 1383 868 3774
Fragments arranged by size:
3774 1383 868
SspI AAT'ATT
Cuts at: 5 52 2082 3097 3949 5
Size: 47 2030 1015 852 2081
Fragments arranged by size:
2081 2030 1015 852 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 2499 2803 3464 513
Size: 552 939 273 222 304 661 3074
Fragments arranged by size:
3074 939 661 552 304 273 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4052 4069 4222 4407 4852 325
Size: 1255 2472 17 153 185 445 1498
Fragments arranged by size:
2472 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 4271 5983
Size: 274 80 33 51 1443 42 2252 1712
Cuts at: 5983 96
Size: 138
Fragments arranged by size:
2252 1712 1443 274 138 80 51 42
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 5985 6017 2439
Size: 32 3514 32 2447
Fragments arranged by size:
3514 2447 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2866 1316
Size: 1550 4475
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI