Restriction map for
pFN21AE6322
(Circular) (Six-base) MAPSORT of: pFN21AE6322.seq Check: 525 from: 1 to: 5671
pFN21AE6322 5671 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 3014 1937
Size: 702 375 4594
Fragments arranged by size:
4594 702 375
Acc65I G'GTAC_C
Cuts at: 2709 2992 2709
Size: 283 5388
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3734 5118 5386 1632
Size: 2102 1384 268 1917
Fragments arranged by size:
2102 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 3414 4986 3414
Size: 1572 4099
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 2091 3001 3368 2091
Size: 910 367 4394
Fragments arranged by size:
4394 910 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2291 2709 2992 4447
Size: 325 217 627 239 265 418 283 1455
Cuts at: 4447 618
Size: 1842
Fragments arranged by size:
1842 1455 627 418 325 283 265 239
217
BanII G_rGCy'C
Cuts at: 1391 2590 2682 2990 1391
Size: 1199 92 308 4072
Fragments arranged by size:
4072 1199 308 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2164 2966 928
Size: 971 265 802 3633
Fragments arranged by size:
3633 971 802 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2629 2663 3862 3896 1903
Size: 34 692 34 1199 34 3678
Fragments arranged by size:
3678 1199 692 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 2187 1548
Size: 639 5032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 4282 136
Size: 107 122 71 631 605 363 2247 1525
Fragments arranged by size:
2247 1525 631 605 363 122 107 71
BglII A'GATC_T
Cuts at: 2208 5666 2208
Size: 3458 2213
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2758 2903 3732 4888 1790
Size: 241 727 145 829 1156 2573
Fragments arranged by size:
2573 1156 829 727 241 145
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2366 2682 3402
Size: 416 53 83 399 24 949 316 720
Cuts at: 3402 4360 442
Size: 958 1753
Fragments arranged by size:
1753 958 949 720 416 399 316 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2543
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2287 2472 4331 1239
Size: 1048 185 1859 2579
Fragments arranged by size:
2579 1859 1048 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2713 3796 4661 4959 2713
Size: 1083 865 298 3425
Fragments arranged by size:
3425 1083 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2305 4334 882
Size: 617 806 2029 2219
Fragments arranged by size:
2219 2029 806 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 2213 5665 2213
Size: 3452 2219
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 2650 4874 5370 2650
Size: 2224 496 2951
Fragments arranged by size:
2951 2224 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 3121 3882 4910 1540
Size: 275 186 34 1086 761 1028 2301
Fragments arranged by size:
2301 1086 1028 761 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 2682 3732 3817 4888 2682
Size: 1050 85 1071 3465
Fragments arranged by size:
3465 1071 1050 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 2138
Size: 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2590 2682 2758 2903 2990 3732
Size: 399 241 559 92 76 145 87 742
Cuts at: 3732 3817 4888 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 241 145 92
87 85 76
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 3026 844
Size: 1972 210 3489
Fragments arranged by size:
3489 1972 210
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 3558 1394
Size: 429 227 1508 3507
Fragments arranged by size:
3507 1508 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 2642 4166 4340 65
Size: 1183 1394 1524 174 1396
Fragments arranged by size:
1524 1396 1394 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 4315 4502 1700
Size: 110 224 2281 187 2869
Fragments arranged by size:
2869 2281 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 2469 96
Size: 2373 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 2389 1184
Size: 1205 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 2758 2758
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2091 2208 3001 3368 3753 3770 5222 5308
Size: 119 117 793 367 385 17 1452 86
Cuts at: 5308 5320 5666 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 793 385 367 346 119 117
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 3889
Fragments arranged by size:
3889 939 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 2897 3187 3981 4001 2229
Size: 668 290 794 20 3899
Fragments arranged by size:
3899 794 668 290 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 3118 4007
Size: 54 1425 50 275 18 588 700 889
Cuts at: 4007 5636 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 700 588 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2870 3601 1353
Size: 1517 731 3423
Fragments arranged by size:
3423 1517 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2680 2680
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2472 3734 4331 5118 5386 1239
Size: 393 655 185 1262 597 787 268 1524
Fragments arranged by size:
1524 1262 787 655 597 393 268 185
EcoO109I rG'GnC_Cy
Cuts at: 2344 2344
Size: 5671
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 4818 1678
Size: 3140 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373
Size: 32 135 32 2740 32 42 32 2626
Fragments arranged by size:
2740 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2329 2640 3015 3261 669
Size: 988 281 391 311 375 246 3079
Fragments arranged by size:
3079 988 391 375 311 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2713 2996 2713
Size: 283 5388
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 4785 4969 1305
Size: 1326 2154 184 2007
Fragments arranged by size:
2154 2007 1326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 10
Size: 54 1425 343 588 3261
Fragments arranged by size:
3261 1425 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3629 3988 4147 518
Size: 648 1611 852 359 159 2042
Fragments arranged by size:
2042 1611 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3087 3764 4912 5157 1606
Size: 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 3889
Fragments arranged by size:
3889 939 552 291
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2584 3029 4574 1132
Size: 97 1355 445 1545 2229
Fragments arranged by size:
2229 1545 1355 445 97
PasI CC'CwG_GG
Cuts at: 1348 2758 1348
Size: 1410 4261
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2892 1108
Size: 1784 3887
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2344 2344
Size: 5671
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2100 3023 830
Size: 1270 923 3478
Fragments arranged by size:
3478 1270 923
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SacI G_AGCT'C
Cuts at: 2682 2682
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 2638 3013 1936
Size: 702 375 4594
Fragments arranged by size:
4594 702 375
SbfI CC_TGCA'GG
Cuts at: 2100 3023 2100
Size: 923 4748
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2096 2318 3019 4154 4835 5026 826
Size: 219 1051 222 701 1135 681 191 1471
Fragments arranged by size:
1471 1135 1051 701 681 222 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2692 3775 4676 4938 820
Size: 197 926 749 1083 901 262 1553
Fragments arranged by size:
1553 1083 926 901 749 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 2347 2383 3110 513
Size: 552 939 291 52 36 727 3074
Fragments arranged by size:
3074 939 727 552 291 52 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 2279 2884 3698 3715 3868 4053
Size: 1255 471 228 605 814 17 153 185
Cuts at: 4053 4498 325
Size: 445 1498
Fragments arranged by size:
1498 1255 814 605 471 445 228 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 3917 5629
Size: 274 80 33 51 1443 492 1448 1712
Cuts at: 5629 96
Size: 138
Fragments arranged by size:
1712 1448 1443 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2893 2893
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI