Restriction map for
pFN21AE6321
(Circular) (Six-base) MAPSORT of: pFN21AE6321.seq Check: 6296 from: 1 to: 5716
pFN21AE6321 5716 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 2643 3037 2643
Size: 394 5322
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2778 2954 3779 5163 5431 1632
Size: 1146 176 825 1384 268 1917
Fragments arranged by size:
1917 1384 1146 825 268 176
AflII C'TTAA_G
Cuts at: 820 1017 2042 820
Size: 197 1025 4494
Fragments arranged by size:
4494 1025 197
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 2480 2483 2840 2953 3459 5031 2480
Size: 3 357 113 506 1572 3165
Fragments arranged by size:
3165 1572 506 357 113 3
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 3025 3251 3403 5512 1493
Size: 1532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2416 2567 2643 3037
Size: 325 217 627 552 77 151 76 394
Cuts at: 3037 4492 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 552 394 325 217 151
77 76
BanII G_rGCy'C
Cuts at: 1391 2160 2542 2947 3035 1391
Size: 769 382 405 88 4072
Fragments arranged by size:
4072 769 405 382 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4745
BbvCI CC'TCA_GC
Cuts at: 2925 2925
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2308 2536 3777 4933 1790
Size: 518 228 1241 1156 2573
Fragments arranged by size:
2573 1241 1156 518 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2862 2975 4376 1239
Size: 1623 113 1401 2579
Fragments arranged by size:
2579 1623 1401 113
Bpu10I CC'TnA_GC
Cuts at: 2925 2946 2925
Size: 21 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2921 3841 4706 5004 2300
Size: 621 920 865 298 3012
Fragments arranged by size:
3012 920 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 2390 2942 1186
Size: 1144 60 552 3960
Fragments arranged by size:
3960 1144 552 60
BseYI C'CCAG_C
Cuts at: 2489 4919 5415 2489
Size: 2430 496 2790
Fragments arranged by size:
2790 2430 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2853 1163
Size: 573 1117 4026
Fragments arranged by size:
4026 1117 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2448 3777 3862 4933 2160
Size: 288 1329 85 1071 2943
Fragments arranged by size:
2943 1329 1071 288 85
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2308 2448 2536 2542 2947 3035
Size: 399 370 148 140 88 6 405 88
Cuts at: 3035 3777 3862 4933 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 370 148 140
88 88 85 6
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 2196 3605 5335 2196
Size: 1409 1730 2577
Fragments arranged by size:
2577 1730 1409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2317 2507 3071 844
Size: 1473 190 564 3489
Fragments arranged by size:
3489 1473 564 190
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2346 2811 4211 4385 65
Size: 1183 1098 465 1400 174 1396
Fragments arranged by size:
1400 1396 1183 1098 465 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 2535 3776 4788 2535
Size: 1241 1012 3463
Fragments arranged by size:
3463 1241 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2480 794
Size: 1686 4030
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2827 2827
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 2982 3046 3413 3798 3815 5267 5353 5365
Size: 1010 64 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1010 385 367 346 86 64
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2246 2509 3232 4026 4046 2246
Size: 263 723 794 20 3916
Fragments arranged by size:
3916 794 723 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681
Size: 54 1425 50 275 18 1333 889 1629
Cuts at: 5681 8
Size: 43
Fragments arranged by size:
1629 1425 1333 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3646 1353
Size: 2293 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2158 2158
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2778 2862 2954 2975 3779 4376 5163
Size: 393 1146 84 92 21 804 597 787
Cuts at: 5163 5431 1239
Size: 268 1524
Fragments arranged by size:
1524 1146 804 787 597 393 268 92
84 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2709 2312
Size: 397 5319
EcoO109I rG'GnC_Cy
Cuts at: 2792 2792
Size: 5716
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373
Size: 32 135 32 2785 32 42 32 2626
Fragments arranged by size:
2785 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3060 3306 669
Size: 988 281 697 425 246 3079
Fragments arranged by size:
3079 988 697 425 281 246
HindIII A'AGCT_T
Cuts at: 748 2177 748
Size: 1429 4287
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 2647 3041 2647
Size: 394 5322
MfeI C'AATT_G
Cuts at: 2638 3315 2638
Size: 677 5039
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2388 4830 5014 1305
Size: 1083 2442 184 2007
Fragments arranged by size:
2442 2007 1083 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2459 2825 3674 4033 4192 518
Size: 648 1029 264 366 849 359 159 2042
Fragments arranged by size:
2042 1029 849 648 366 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2480 2756 2924 3132 3809 4957 5202 1606
Size: 874 276 168 208 677 1148 245 2120
Fragments arranged by size:
2120 1148 874 677 276 245 208 168
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 2881 387
Size: 2494 3222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2738 3074 4619 1132
Size: 97 1509 336 1545 2229
Fragments arranged by size:
2229 1545 1509 336 97
PasI CC'CwG_GG
Cuts at: 1348 2139 1348
Size: 791 4925
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 2504 3000 3068 830
Size: 1674 496 68 3478
Fragments arranged by size:
3478 1674 496 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2480 2756 2924 2480
Size: 276 168 5272
Fragments arranged by size:
5272 276 168
SacI G_AGCT'C
Cuts at: 2160 2160
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 2500 2996 3064 4199 4880 5071 826
Size: 219 1455 496 68 1135 681 191 1471
Fragments arranged by size:
1471 1455 1135 681 496 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2279 2900 3820 4721 4983
Size: 197 926 99 237 621 920 901 262
Cuts at: 4983 820
Size: 1553
Fragments arranged by size:
1553 926 920 901 621 262 237 197
99
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 2515 152
Size: 2363 3353
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 3155 513
Size: 552 2090 3074
Fragments arranged by size:
3074 2090 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2591 2629 2819 3743 3760 3913 4098
Size: 1255 1011 38 190 924 17 153 185
Cuts at: 4098 4543 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1011 924 445 190 185 153
38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3962 5674 96
Size: 274 80 33 51 1443 1985 1712 138
Fragments arranged by size:
1985 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2287 2319 2662 2694 5676 5708 2287
Size: 32 343 32 2982 32 2295
Fragments arranged by size:
2982 2295 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2353
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 2114 3845 2114
Size: 1731 3985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI