Restriction map for
pFN21AE6315
(Circular) (Six-base) MAPSORT of: pFN21AE6315.seq Check: 4762 from: 1 to: 6016
pFN21AE6315 6016 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2161 2928 4079 5463 5731 1632
Size: 529 767 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1151 767 529 268
AfeI AGC'GCT
Cuts at: 2433 2433
Size: 6016
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 2322 3087 4915 5892 1203
Size: 1119 765 1828 977 1327
Fragments arranged by size:
1828 1327 1119 977 765
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 3000 4745 3000
Size: 1745 4271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2369 2452 1184
Size: 1185 83 4748
Fragments arranged by size:
4748 1185 83
AlwNI CAG_nnn'CTG
Cuts at: 3759 5331 3759
Size: 1572 4444
ApaI G_GGCC'C
Cuts at: 2093 2093
Size: 6016
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3325 3551 3703 5812 1493
Size: 1832 226 152 2109 1697
Fragments arranged by size:
2109 1832 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 3341 1870
Size: 73 210 1188 4545
Fragments arranged by size:
4545 1188 210 73
AvrII C'CTAG_G
Cuts at: 2704 2704
Size: 6016
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2272 2333 3337 4792
Size: 325 217 627 281 204 61 1004 1455
Cuts at: 4792 618
Size: 1842
Fragments arranged by size:
1842 1455 1004 627 325 281 217 204
61
BanII G_rGCy'C
Cuts at: 1391 2093 2148 2208 3335 1391
Size: 702 55 60 1127 4072
Fragments arranged by size:
4072 1127 702 60 55
BbeI G_GCGC'C
Cuts at: 2072 2276 2337 2072
Size: 204 61 5751
Fragments arranged by size:
5751 204 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 928
Size: 971 853 4192
Fragments arranged by size:
4192 971 853
BbvCI CC'TCA_GC
Cuts at: 2591 2591
Size: 6016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2086 2120 2387 2421 4207 4241 1903
Size: 34 149 34 267 34 1786 34 3678
Fragments arranged by size:
3678 1786 267 149 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2900 3908 5118 2900
Size: 1008 1210 3798
Fragments arranged by size:
3798 1210 1008
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2226 2445 2510
Size: 107 122 71 631 605 554 219 65
Cuts at: 2510 4627 136
Size: 2117 1525
Fragments arranged by size:
2117 1525 631 605 554 219 122 107
71 65
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 2147 3433 2147
Size: 1286 4730
Bme1580I G_kGCm'C
Cuts at: 1790 2093 4077 5233 1790
Size: 303 1984 1156 2573
Fragments arranged by size:
2573 1984 1156 303
BmgBI CAC'GTC
Cuts at: 1318 2380 2401 1318
Size: 1062 21 4933
Fragments arranged by size:
4933 1062 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3747 4705 442
Size: 416 53 83 399 24 2330 958 1753
Fragments arranged by size:
2330 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2107 4676 1239
Size: 868 2569 2579
Fragments arranged by size:
2579 2569 868
Bpu10I CC'TnA_GC
Cuts at: 2591 2591
Size: 6016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4141 5006 5304 4141
Size: 865 298 4853
Fragments arranged by size:
4853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2823 4679 882
Size: 617 1324 1856 2219
Fragments arranged by size:
2219 1856 1324 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2273 2334 2757
Size: 53 83 186 936 536 204 61 423
Cuts at: 2757 3823 4205 4840 275
Size: 1066 382 635 1451
Fragments arranged by size:
1451 1066 936 635 536 423 382 204
186 83 61 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5791
Fragments arranged by size:
5791 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2602 1186
Size: 1416 4600
BseYI C'CCAG_C
Cuts at: 2356 2664 5219 5715 2356
Size: 308 2555 496 2657
Fragments arranged by size:
2657 2555 496 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2353 2711 3047 1163
Size: 573 617 358 336 4132
Fragments arranged by size:
4132 617 573 358 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 4077 4162 5233 2520
Size: 1557 85 1071 3303
Fragments arranged by size:
3303 1557 1071 85
BsmI GAATG_Cn'
Cuts at: 3527 3620 3527
Size: 93 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2148 2208 2520 3335 4077 4162
Size: 399 303 55 60 312 815 742 85
Cuts at: 4162 5233 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 815 742 399 312 303 85
60 55
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 2601 3905 5635 2601
Size: 1304 1730 2982
Fragments arranged by size:
2982 1730 1304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3371 844
Size: 2527 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3302 3903 1394
Size: 429 1479 601 3507
Fragments arranged by size:
3507 1479 601 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2855 4660 4847 1700
Size: 110 319 726 1805 187 2869
Fragments arranged by size:
2869 1805 726 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 3035 3084 96
Size: 2939 49 3028
Fragments arranged by size:
3028 2939 49
BssHII G'CGCG_C
Cuts at: 1925 2418 1925
Size: 493 5523
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2623 2086
Size: 537 5479
BstYI r'GATC_y
Cuts at: 1972 2655 3184 3346 3713 4098 4115 5567 5653
Size: 683 529 162 367 385 17 1452 86
Cuts at: 5653 5665 6011 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 683 529 385 367 346 162
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2242 2311 2412 2508 513
Size: 552 939 238 69 101 96 4021
Fragments arranged by size:
4021 939 552 238 101 96 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 3532 4326 4346 3532
Size: 794 20 5202
Fragments arranged by size:
5202 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 3463 4352
Size: 54 1425 50 275 18 672 961 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 961 889 672 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2972 3231 3946 1353
Size: 686 933 259 715 3423
Fragments arranged by size:
3423 933 715 686 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2161 2928 4079 4676 5463 5731
Size: 393 475 54 767 1151 597 787 268
Cuts at: 5731 1239
Size: 1524
Fragments arranged by size:
1524 1151 787 767 597 475 393 268
54
EcoNI CCTnn'n_nnAGG
Cuts at: 2931 2931
Size: 6016
EcoO109I rG'GnC_Cy
Cuts at: 2090 2870 2090
Size: 780 5236
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2072 2130 2276 2337 2435 5163 1678
Size: 394 58 146 61 98 2728 2531
Fragments arranged by size:
2728 2531 394 146 98 61 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2707 2739 2986 3018 4657
Size: 32 135 32 1135 32 247 32 1639
Cuts at: 4657 4689 4731 4763 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1639 1135 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 3360 3606 669
Size: 988 281 637 785 246 3079
Fragments arranged by size:
3079 988 785 637 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KasI G'GCGC_C
Cuts at: 2068 2272 2333 2068
Size: 204 61 5751
Fragments arranged by size:
5751 204 61
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2219 5130 5314 1305
Size: 914 2911 184 2007
Fragments arranged by size:
2911 2007 914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2600 3086 3974 4333 4492 518
Size: 648 1185 249 486 888 359 159 2042
Fragments arranged by size:
2042 1185 888 648 486 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2217 2305 2458 2664 2900 3432 4109 5257
Size: 611 88 153 206 236 532 677 1148
Cuts at: 5257 5502 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 611 532 245 236 206
153 88
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 6016
NarI GG'CG_CC
Cuts at: 2069 2273 2334 2069
Size: 204 61 5751
Fragments arranged by size:
5751 204 61
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4525
Fragments arranged by size:
4525 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 3085 3091 3374 4919 1132
Size: 97 1856 6 283 1545 2229
Fragments arranged by size:
2229 1856 1545 283 97 6
PasI CC'CwG_GG
Cuts at: 1348 2141 2268 1348
Size: 793 127 5096
Fragments arranged by size:
5096 793 127
PciI A'CATG_T
Cuts at: 3087 4915 3087
Size: 1828 4188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2870 2870
Size: 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3222 3586 3222
Size: 364 5652
PspOMI G'GGCC_C
Cuts at: 2089 2089
Size: 6016
PstI C_TGCA'G
Cuts at: 830 2732 2899 3124 3368 830
Size: 1902 167 225 244 3478
Fragments arranged by size:
3478 1902 244 225 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2664 2900 2664
Size: 236 5780
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2039 2039
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2659 2728 2895 3120 3364 4499 5180
Size: 219 1614 69 167 225 244 1135 681
Cuts at: 5180 5371 826
Size: 191 1471
Fragments arranged by size:
1614 1471 1135 681 244 225 219 191
167 69
SfoI GGC'GCC
Cuts at: 2070 2274 2335 2070
Size: 204 61 5751
Fragments arranged by size:
5751 204 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4120 5021 5283 820
Size: 197 926 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6016
StyI C'CwwG_G
Cuts at: 513 1065 2004 2704 3455 513
Size: 552 939 700 751 3074
Fragments arranged by size:
3074 939 751 700 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2995 4043 4060 4213 4398 4843 325
Size: 1255 1415 1048 17 153 185 445 1498
Fragments arranged by size:
1498 1415 1255 1048 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3035 3084 4262
Size: 274 80 33 51 1443 1058 49 1178
Cuts at: 4262 5974 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1178 1058 274 138 80 51
49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 5976 6008 2234
Size: 32 3710 32 2242
Fragments arranged by size:
3710 2242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3190 3352 3190
Size: 162 5854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 3049 2359
Size: 690 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2324 2387 4145 2324
Size: 63 1758 4195
Fragments arranged by size:
4195 1758 63
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI