Restriction map for
pFN21AE6312
(Circular) (Six-base) MAPSORT of: pFN21AE6312.seq Check: 3463 from: 1 to: 5647
pFN21AE6312 5647 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 2381
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2387 2815 3710 5094 5362 1632
Size: 608 147 428 895 1384 268 1917
Fragments arranged by size:
1917 1384 895 608 428 268 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2673 1166
Size: 1507 4140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 5615
AlwNI CAG_nnn'CTG
Cuts at: 2128 3390 4962 2128
Size: 1262 1572 2813
Fragments arranged by size:
2813 1572 1262
ApaI G_GGCC'C
Cuts at: 2876 2876
Size: 5647
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2520 2571 2972 1870
Size: 73 235 342 51 401 4545
Fragments arranged by size:
4545 401 342 235 73 51
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2393 2402 2968 4423 618
Size: 325 217 627 606 9 566 1455 1842
Fragments arranged by size:
1842 1455 627 606 566 325 217 9
BanII G_rGCy'C
Cuts at: 1391 2876 2966 1391
Size: 1485 90 4072
Fragments arranged by size:
4072 1485 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2225 3539 4749 2225
Size: 1314 1210 3123
Fragments arranged by size:
3123 1314 1210
BclI T'GATC_A
Cuts at: 1548 2685 1548
Size: 1137 4510
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2238 2429
Size: 107 122 71 631 605 337 229 191
Cuts at: 2429 4258 136
Size: 1829 1525
Fragments arranged by size:
1829 1525 631 605 337 229 191 122
107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2876 3708 4864 1790
Size: 1086 832 1156 2573
Fragments arranged by size:
2573 1156 1086 832
BmgBI CAC'GTC
Cuts at: 1318 2797 1318
Size: 1479 4168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2259 2343 3378
Size: 416 53 83 399 24 842 84 1035
Cuts at: 3378 4336 442
Size: 958 1753
Fragments arranged by size:
1753 1035 958 842 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4307 1239
Size: 3068 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 2513 5641 2513
Size: 3128 2519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2145 4080 4478 4752 4899 5310 2145
Size: 1935 398 274 147 411 2482
Fragments arranged by size:
2482 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 1178
Size: 30 165 30 694 30 4698
Fragments arranged by size:
4698 694 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 2595 4850 5346 2595
Size: 2255 496 2896
Fragments arranged by size:
2896 2255 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5074
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
BsmBI CGTCTCn'nnnn_
Cuts at: 2936 2936
Size: 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2876 2966 3708 3793 4864 1391
Size: 399 1086 90 742 85 1071 2174
Fragments arranged by size:
2174 1086 1071 742 399 90 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 3002 844
Size: 1537 621 3489
Fragments arranged by size:
3489 1537 621
BsrBI CCG'CTC
Cuts at: 1394 1823 2160 3534 1394
Size: 429 337 1374 3507
Fragments arranged by size:
3507 1374 429 337
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 4142 4316 65
Size: 1183 1479 1415 174 1396
Fragments arranged by size:
1479 1415 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2457 4291 4478 1700
Size: 110 220 427 1834 187 2869
Fragments arranged by size:
2869 1834 427 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 2512 4451 2512
Size: 1939 3708
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2722 2480
Size: 242 5405
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2234 2234
Size: 5647
BtgI C'CryG_G
Cuts at: 513 1065 2004 2479 2565 513
Size: 552 939 475 86 3595
Fragments arranged by size:
3595 939 552 475 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2703 3163 3957 3977 2703
Size: 460 794 20 4373
Fragments arranged by size:
4373 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DraIII CAC_nnn'GTG
Cuts at: 2544 2544
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2455 3094
Size: 54 1425 50 275 18 264 361 639
Cuts at: 3094 3983 5612 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 639 361 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 2939 3577 1353
Size: 1167 419 638 3423
Fragments arranged by size:
3423 1167 638 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2387 2815 3710 4307 5094 5362
Size: 393 608 147 428 895 597 787 268
Cuts at: 5362 1239
Size: 1524
Fragments arranged by size:
1524 895 787 608 597 428 393 268
147
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 2840
Size: 5647
EcoO109I rG'GnC_Cy
Cuts at: 2089 2873 2089
Size: 784 4863
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2450 2644 2950 2450
Size: 194 306 5147
Fragments arranged by size:
5147 306 194
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2329 2479 4794 1678
Size: 651 150 2315 2531
Fragments arranged by size:
2531 2315 651 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2677 2709 4288 4320 4362
Size: 32 135 32 1105 32 1579 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1579 1105 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2673 2720 2756 3605 3964 4123 518
Size: 648 1507 47 36 849 359 159 2042
Fragments arranged by size:
2042 1507 849 648 359 159 47 36
MspA1I CmG'CkG
Cuts at: 1606 2468 2691 3063 3740 4888 5133 1606
Size: 862 223 372 677 1148 245 2120
Fragments arranged by size:
2120 1148 862 677 372 245 223
NaeI GCC'GGC
Cuts at: 2032 2459 2032
Size: 427 5220
NcoI C'CATG_G
Cuts at: 513 1065 2004 2565 513
Size: 552 939 561 3595
Fragments arranged by size:
3595 939 561 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NgoMIV G'CCGG_C
Cuts at: 2030 2457 2030
Size: 427 5220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2755 3005 4550 1132
Size: 97 1526 250 1545 2229
Fragments arranged by size:
2229 1545 1526 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 1108
Size: 1020 4627
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2872 2872
Size: 5647
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 5615
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2468 2468
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SexAI A'CCwGG_T
Cuts at: 2133 2133
Size: 5647
SfcI C'TryA_G
Cuts at: 826 1045 2109 2995 4130 4811 5002 826
Size: 219 1064 886 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 886 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2522 2974 1872
Size: 650 452 4545
Fragments arranged by size:
4545 650 452
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 3751 4652 4914 820
Size: 197 926 235 1573 901 262 1553
Fragments arranged by size:
1573 1553 926 901 262 235 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2232 2921 1517
Size: 715 689 4243
Fragments arranged by size:
4243 715 689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2299 2565 3086 513
Size: 552 939 295 266 521 3074
Fragments arranged by size:
3074 939 552 521 295 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3893 5605
Size: 274 80 33 51 1443 207 1709 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1709 1443 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 2178 1943
Size: 235 5412
XmaI C'CCGG_G
Cuts at: 1870 2520 2972 1870
Size: 650 452 4545
Fragments arranged by size:
4545 650 452
XmnI GAAnn'nnTTC
Cuts at: 2846 3776 2846
Size: 930 4717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI