Restriction map for
pFN21AE6311
(Circular) (Six-base) MAPSORT of: pFN21AE6311.seq Check: 9940 from: 1 to: 5980
pFN21AE6311 5980 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4043 5427 5695 1632
Size: 2411 1384 268 1917
Fragments arranged by size:
2411 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2504 1184
Size: 1320 4660
AlwNI CAG_nnn'CTG
Cuts at: 2998 3160 3193 3723 5295 2998
Size: 162 33 530 1572 3683
Fragments arranged by size:
3683 1572 530 162 33
ApaI G_GGCC'C
Cuts at: 2569 3214 2569
Size: 645 5335
ApaLI G'TGCA_C
Cuts at: 2179 2909 4037 5193 2179
Size: 730 1128 1156 2966
Fragments arranged by size:
2966 1156 1128 730
ApoI r'AATT_y
Cuts at: 1493 3289 3515 3667 5776 1493
Size: 1796 226 152 2109 1697
Fragments arranged by size:
2109 1796 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2112 2842 3176 3305 1870
Size: 73 169 730 334 129 4545
Fragments arranged by size:
4545 730 334 169 129 73
BamHI G'GATC_C
Cuts at: 2553 2890 3310 3677 2553
Size: 337 420 367 4856
Fragments arranged by size:
4856 420 367 337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2669 3301 4756 618
Size: 325 217 627 477 405 632 1455 1842
Fragments arranged by size:
1842 1455 632 627 477 405 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 2569 3214 3299 1391
Size: 630 548 645 85 4072
Fragments arranged by size:
4072 645 630 548 85
BbeI G_GCGC'C
Cuts at: 2268 2268
Size: 5980
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 928
Size: 971 317 4692
Fragments arranged by size:
4692 971 317
BbvCI CC'TCA_GC
Cuts at: 2068 2068
Size: 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 5122
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2393 4591
Size: 107 122 71 631 605 406 315 2198
Cuts at: 4591 136
Size: 1525
Fragments arranged by size:
2198 1525 631 605 406 315 122 107
71
BglII A'GATC_T
Cuts at: 2688 5975 2688
Size: 3287 2693
BlpI GC'TnA_GC
Cuts at: 2588 3397 2588
Size: 809 5171
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2183 2569 2913 3214 4041 5197 1790
Size: 271 122 386 344 301 827 1156 2573
Fragments arranged by size:
2573 1156 827 386 344 301 271 122
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2441 2471 2540 3125 3182 4640 1239
Size: 920 282 30 69 585 57 1458 2579
Fragments arranged by size:
2579 1458 920 585 282 69 57 30
Bpu10I CC'TnA_GC
Cuts at: 2068 2068
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 2804 3123 5974 2804
Size: 319 2851 2810
Fragments arranged by size:
2851 2810 319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2265 3787 4169
Size: 53 83 186 936 476 256 1522 382
Cuts at: 4169 4804 275
Size: 635 1451
Fragments arranged by size:
1522 1451 936 635 476 382 256 186
83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2143 2150 2173 1178
Size: 30 165 30 717 23 7 23 4985
Fragments arranged by size:
4985 717 165 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5980
BseYI C'CCAG_C
Cuts at: 2341 2528 2703 3229 5183 5679 2341
Size: 187 175 526 1954 496 2642
Fragments arranged by size:
2642 1954 526 496 187 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2529 1163
Size: 573 408 385 4614
Fragments arranged by size:
4614 573 408 385
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2183 2427 2913 3019 4041 4126 5197 2183
Size: 244 486 106 1022 85 1071 2966
Fragments arranged by size:
2966 1071 1022 486 244 106 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2061 2183 2427 2569 2913 3019
Size: 399 231 40 122 244 142 344 106
Cuts at: 3019 3214 3299 4041 4126 5197 1391
Size: 195 85 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 244 231 195
142 122 106 85 85 40
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2169 3200 4624 4811 1700
Size: 110 359 1031 1424 187 2869
Fragments arranged by size:
2869 1424 1031 359 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 794
Size: 1919 4061
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 3222 3222
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2553 2688 2890 3157 3310 3677 4062 4079
Size: 581 135 202 267 153 367 385 17
Cuts at: 4079 5531 5617 5629 5975 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 581 385 367 346 267 202
153 135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2859 3082 513
Size: 552 939 855 223 3411
Fragments arranged by size:
3411 939 855 552 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 4290 4310 3496
Size: 794 20 5166
Fragments arranged by size:
5166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2806 2841 529
Size: 35 2242 35 3668
Fragments arranged by size:
3668 2242 35 35
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 2221 2221
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2048 4987 809
Size: 507 732 2939 1802
Fragments arranged by size:
2939 1802 732 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2862 3198 3427
Size: 54 1425 50 275 18 1032 336 229
Cuts at: 3427 4316 5945 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 336 275 229 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3245 3251 3910 1353
Size: 1892 6 659 3423
Fragments arranged by size:
3423 1892 659 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2441 2471 2540 3125 3182 4043
Size: 393 527 282 30 69 585 57 861
Cuts at: 4043 4640 5427 5695 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 861 787 597 585 527 393 282
268 69 57 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2885 3242 2885
Size: 357 5623
EcoO109I rG'GnC_Cy
Cuts at: 2565 2986 3211 2565
Size: 421 225 5334
Fragments arranged by size:
5334 421 225
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 5980
FspI TGC'GCA
Cuts at: 2717 4486 2717
Size: 1769 4211
FspAI rTGC'GCAy
Cuts at: 2717 2717
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2268 5127 1678
Size: 590 2859 2531
Fragments arranged by size:
2859 2531 590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4621 4653 4695 4727 1373
Size: 32 135 32 3049 32 42 32 2626
Fragments arranged by size:
3049 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KasI G'GCGC_C
Cuts at: 2264 2264
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2604 5094 5278 1305
Size: 1299 2490 184 2007
Fragments arranged by size:
2490 2007 1299 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 3938 4297 4456 518
Size: 648 1026 1746 359 159 2042
Fragments arranged by size:
2042 1746 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2528 2587 3396 4073 5221 5466 1606
Size: 922 59 809 677 1148 245 2120
Fragments arranged by size:
2120 1148 922 809 677 245 59
NaeI GCC'GGC
Cuts at: 2171 3202 2171
Size: 1031 4949
NarI GG'CG_CC
Cuts at: 2265 2265
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 2169 3200 2169
Size: 1031 4949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2405 3338 4883 1132
Size: 97 1176 933 1545 2229
Fragments arranged by size:
2229 1545 1176 933 97
PasI CC'CwG_GG
Cuts at: 1348 3100 1348
Size: 1752 4228
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2652 1108
Size: 1544 4436
PfoI T'CCnGG_A
Cuts at: 2556 2556
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspOMI G'GGCC_C
Cuts at: 2565 3210 2565
Size: 645 5335
PspXI vC'TCGA_Gb
Cuts at: 3176 3176
Size: 5980
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2528 2587 2528
Size: 59 5921
RsrII CG'GwC_CG
Cuts at: 2104 2104
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 3328 4463 5144 5335 826
Size: 219 2283 1135 681 191 1471
Fragments arranged by size:
2283 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2266 2266
Size: 5980
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2114 3307 1872
Size: 242 1193 4545
Fragments arranged by size:
4545 1193 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3176 4084 4985 5247 820
Size: 197 926 1233 908 901 262 1553
Fragments arranged by size:
1553 1233 926 908 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 3174 3248 1517
Size: 879 778 74 4249
Fragments arranged by size:
4249 879 778 74
StyI C'CwwG_G
Cuts at: 513 1065 2004 2646 2730 3419 513
Size: 552 939 642 84 689 3074
Fragments arranged by size:
3074 939 689 642 552 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2121 2503 4007 4024 4177 4362 4807
Size: 1255 541 382 1504 17 153 185 445
Cuts at: 4807 325
Size: 1498
Fragments arranged by size:
1504 1498 1255 541 445 382 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 2538 2570 5940 5972 2143
Size: 32 363 32 3370 32 2151
Fragments arranged by size:
3370 2151 363 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2591 2866 2591
Size: 275 5705
XhoI C'TCGA_G
Cuts at: 1943 3176 1943
Size: 1233 4747
XmaI C'CCGG_G
Cuts at: 1870 2112 3305 1870
Size: 242 1193 4545
Fragments arranged by size:
4545 1193 242
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI
NsiI PacI PmlI PpuMI PsrI SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI