Restriction map for
pFN21AE6310
(Circular) (Six-base) MAPSORT of: pFN21AE6310.seq Check: 5605 from: 1 to: 5956
pFN21AE6310 5956 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2514 3299 1937
Size: 577 785 4594
Fragments arranged by size:
4594 785 577
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2406 4019 5403 5671 1632
Size: 774 1613 1384 268 1917
Fragments arranged by size:
1917 1613 1384 774 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaI G_GGCC'C
Cuts at: 2409 2409
Size: 5956
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2786 3077 3265 3491 3643 5752 1493
Size: 1293 291 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1293 291 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 3277 4732 618
Size: 325 217 627 578 912 1455 1842
Fragments arranged by size:
1842 1455 912 627 578 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 2409 3275 1391
Size: 715 303 866 4072
Fragments arranged by size:
4072 866 715 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2753 928
Size: 971 854 4131
Fragments arranged by size:
4131 971 854
BbvCI CC'TCA_GC
Cuts at: 2092 2969 2092
Size: 877 5079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2344 3848 5058 2344
Size: 1504 1210 3242
Fragments arranged by size:
3242 1504 1210
BclI T'GATC_A
Cuts at: 1548 2875 1548
Size: 1327 4629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2696 4567 136
Size: 107 122 71 631 605 1024 1871 1525
Fragments arranged by size:
1871 1525 1024 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2368 2409 2856 4017 5173 1790
Size: 578 41 447 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 578 447 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 2403 3687
Size: 416 53 83 399 24 954 32 1284
Cuts at: 3687 4645 442
Size: 958 1753
Fragments arranged by size:
1753 1284 958 954 416 399 83 53
32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4616 1239
Size: 3377 2579
Bpu10I CC'TnA_GC
Cuts at: 2030 2092 2715 2969 2030
Size: 62 623 254 5017
Fragments arranged by size:
5017 623 254 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2310 2990 4619 882
Size: 617 811 680 1629 2219
Fragments arranged by size:
2219 1629 811 680 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 2488 1186
Size: 1110 192 4654
Fragments arranged by size:
4654 1110 192
BseYI C'CCAG_C
Cuts at: 2051 2168 2900 5159 5655 2051
Size: 117 732 2259 496 2352
Fragments arranged by size:
2352 2259 732 496 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3231 4017 4102 5173 3231
Size: 786 85 1071 4014
Fragments arranged by size:
4014 1071 786 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
BsmBI CGTCTCn'nnnn_
Cuts at: 3118 3118
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2368 2409 2856 3231 3275 4017
Size: 399 316 262 41 447 375 44 742
Cuts at: 4017 4102 5173 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 375 316 262
85 44 41
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 2878 3845 5575 2878
Size: 967 1730 3259
Fragments arranged by size:
3259 1730 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 844
Size: 2467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 2805 4451 4625 65
Size: 1183 1284 273 1646 174 1396
Fragments arranged by size:
1646 1396 1284 1183 273 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 2553 96
Size: 2457 3499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 3224 4016 5028 3224
Size: 792 1012 4152
Fragments arranged by size:
4152 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 2191
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2562 2591 3286 3653 4038 4055 5507 5593
Size: 590 29 695 367 385 17 1452 86
Cuts at: 5593 5605 5951 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 695 590 385 367 346 86
29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2490 3403
Size: 54 1425 50 275 18 363 297 913
Cuts at: 3403 4292 5921 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 913 889 363 297 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2993 3121 3886 1353
Size: 1640 128 765 3423
Fragments arranged by size:
3423 1640 765 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 4019 4616 5403 5671 1239
Size: 393 774 1613 597 787 268 1524
Fragments arranged by size:
1613 1524 787 774 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3103 3103
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 4597 4629 4671
Size: 32 135 32 746 32 2247 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 2247 746 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 10
Size: 54 1425 343 660 3474
Fragments arranged by size:
3474 1425 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 3914 4273 4432 518
Size: 648 1125 1623 359 159 2042
Fragments arranged by size:
2042 1623 1125 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2968 3372 4049 5197 5442 1606
Size: 445 917 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 445 404 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2321 1348
Size: 973 4983
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5054
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2405 2405
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2051 2968 2051
Size: 917 5039
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SapI GCTCTTCn'nnn_
Cuts at: 2993 2993
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 2127 2680 4204 5916 2127
Size: 553 1524 1712 2167
Fragments arranged by size:
2167 1712 1524 553
SfcI C'TryA_G
Cuts at: 826 1045 2046 2568 2674 3304 4439 5120 5311
Size: 219 1001 522 106 630 1135 681 191
Cuts at: 5311 826
Size: 1471
Fragments arranged by size:
1471 1135 1001 681 630 522 219 191
106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 2057 152
Size: 1905 4051
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2106 2535 3395 513
Size: 552 939 102 429 860 3074
Fragments arranged by size:
3074 939 860 552 429 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2125 2553 2678
Size: 274 80 33 51 1443 148 428 125
Cuts at: 2678 4202 5914 96
Size: 1524 1712 138
Fragments arranged by size:
1712 1524 1443 428 274 148 138 125
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2854 2886 5916 5948 2854
Size: 32 3030 32 2862
Fragments arranged by size:
3030 2862 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2485
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI