Restriction map for
pFN21AE6307
(Circular) (Six-base) MAPSORT of: pFN21AE6307.seq Check: 5833 from: 1 to: 5647
pFN21AE6307 5647 bp
With 182 enzymes: SgfI *
July 1, 2014 12:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 2277 2486 3710 5094 5362 1632
Size: 504 141 209 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 504 268 209 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2519 4546 5523 1203
Size: 1316 2027 977 1327
Fragments arranged by size:
2027 1327 1316 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaI G_GGCC'C
Cuts at: 2266 2266
Size: 5647
ApaLI G'TGCA_C
Cuts at: 2160 3704 4860 2160
Size: 1544 1156 2947
Fragments arranged by size:
2947 1544 1156
ApoI r'AATT_y
Cuts at: 1493 2540 2596 2777 2956 3182 3334 5443 1493
Size: 1047 56 181 179 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 226 181 179 152 56
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2533 2731 2977 3344 2533
Size: 198 246 367 4836
Fragments arranged by size:
4836 367 246 198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2339 2968 4423 618
Size: 325 217 627 221 331 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 331 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2266 2316 2966 1391
Size: 875 50 650 4072
Fragments arranged by size:
4072 875 650 50
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BfrBI ATG'CAT
Cuts at: 2929 2929
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2164 2266 3708 4864 1790
Size: 374 102 1442 1156 2573
Fragments arranged by size:
2573 1442 1156 374 102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 4307 1239
Size: 1013 2055 2579
Fragments arranged by size:
2579 2055 1013
Bpu10I CC'TnA_GC
Cuts at: 2073 2073
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2349 3438 493
Size: 711 1145 1089 2702
Fragments arranged by size:
2702 1145 1089 711
BsaBI GATnn'nnATC
Cuts at: 2730 5641 2730
Size: 2911 2736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3454 3836 4471
Size: 53 83 186 936 476 1445 382 635
Cuts at: 4471 275
Size: 1451
Fragments arranged by size:
1451 1445 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 1178
Size: 30 165 30 924 30 4468
Fragments arranged by size:
4468 924 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 2369 4850 5346 2369
Size: 2481 496 2670
Fragments arranged by size:
2670 2481 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2481 1163
Size: 573 745 4329
Fragments arranged by size:
4329 745 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2164 3708 3793 4864 2164
Size: 1544 85 1071 2947
Fragments arranged by size:
2947 1544 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 2266 2316 2966 3708 3793 4864
Size: 399 374 102 50 650 742 85 1071
Cuts at: 4864 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 650 399 374 102 85
50
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 2414 3002 844
Size: 1164 406 588 3489
Fragments arranged by size:
3489 1164 588 406
BsrBI CCG'CTC
Cuts at: 1394 1823 2310 3534 1394
Size: 429 487 1224 3507
Fragments arranged by size:
3507 1224 487 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 2548 3707 4719 2548
Size: 1159 1012 3476
Fragments arranged by size:
3476 1159 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2843 794
Size: 2049 3598
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2203 2203
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2533 2725 2731 2977 3344 3729 3746 5198
Size: 561 192 6 246 367 385 17 1452
Cuts at: 5198 5284 5296 5642 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 561 385 367 346 246 192
86 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 4114
Fragments arranged by size:
4114 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2316 487
Size: 683 56 1090 3818
Fragments arranged by size:
3818 1090 683 56
BtsI GCAGTG_nn'
Cuts at: 2336 2364 3163 3957 3977 2336
Size: 28 799 794 20 4006
Fragments arranged by size:
4006 799 794 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 3094 3983
Size: 54 1425 50 275 18 276 988 889
Cuts at: 3983 5612 8
Size: 1629 43
Fragments arranged by size:
1629 1425 988 889 276 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2239 2873 3577 1353
Size: 886 634 704 3423
Fragments arranged by size:
3423 886 704 634
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2252 2277 2486 3710 4307 5094
Size: 393 504 116 25 209 1224 597 787
Cuts at: 5094 5362 1239
Size: 268 1524
Fragments arranged by size:
1524 1224 787 597 504 393 268 209
116 25
EcoO109I rG'GnC_Cy
Cuts at: 2020 2262 2263 2020
Size: 242 1 5404
Fragments arranged by size:
5404 242 1
EcoRI G'AATT_C
Cuts at: 2777 2956 3334 2777
Size: 179 378 5090
Fragments arranged by size:
5090 378 179
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2012 4794 1678
Size: 334 2782 2531
Fragments arranged by size:
2782 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373
Size: 32 135 32 2716 32 42 32 2626
Fragments arranged by size:
2716 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 2519 5523 2519
Size: 3004 2643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3605 3964 4123 518
Size: 648 2439 359 159 2042
Fragments arranged by size:
2439 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 3063 3740 4888 5133 1606
Size: 442 1015 677 1148 245 2120
Fragments arranged by size:
2120 1148 1015 677 442 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5647
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NsiI A_TGCA'T
Cuts at: 2931 2931
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2352 1108
Size: 1244 4403
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2349 2349
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2020
Size: 5647
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2262 2262
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SapI GCTCTTCn'nnn_
Cuts at: 2239 2239
Size: 5647
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2995 4130 4811 5002 826
Size: 219 1950 1135 681 191 1471
Fragments arranged by size:
1950 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 3086 513
Size: 552 939 1082 3074
Fragments arranged by size:
3074 1082 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 3674 3691 3844 4029 4474 325
Size: 1255 1329 765 17 153 185 445 1498
Fragments arranged by size:
1498 1329 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2147 3893 5605
Size: 274 80 33 51 1443 170 1746 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1746 1712 1443 274 170 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2841 2873 5607 5639 2841
Size: 32 2734 32 2849
Fragments arranged by size:
2849 2734 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2276 2276
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI
SrfI SwaI