Restriction map for
pFN21AE6304
(Circular) (Six-base) MAPSORT of: pFN21AE6304.seq Check: 833 from: 1 to: 6034
pFN21AE6304 6034 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3267 3296 3267
Size: 29 6005
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 3377 1937
Size: 876 564 4594
Fragments arranged by size:
4594 876 564
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 4097 5481 5749 1632
Size: 621 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 621 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6034
AlwNI CAG_nnn'CTG
Cuts at: 3119 3777 5349 3119
Size: 658 1572 3804
Fragments arranged by size:
3804 1572 658
ApaI G_GGCC'C
Cuts at: 2552 3324 2552
Size: 772 5262
ApaLI G'TGCA_C
Cuts at: 4091 5247 4091
Size: 1156 4878
ApoI r'AATT_y
Cuts at: 1493 2469 3343 3569 3721 5830 1493
Size: 976 874 226 152 2109 1697
Fragments arranged by size:
2109 1697 976 874 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 3018 3052 3359 1870
Size: 73 1075 34 307 4545
Fragments arranged by size:
4545 1075 307 73 34
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2638 3255 3355 4810 618
Size: 325 217 627 851 617 100 1455 1842
Fragments arranged by size:
1842 1455 851 627 617 325 217 100
BanII G_rGCy'C
Cuts at: 1391 2552 2788 2796 3227 3324 3353 1391
Size: 1161 236 8 431 97 29 4072
Fragments arranged by size:
4072 1161 431 236 97 29 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 3015 928
Size: 971 495 621 3947
Fragments arranged by size:
3947 971 621 495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4225 4259 1903
Size: 34 2288 34 3678
Fragments arranged by size:
3678 2288 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4645 136
Size: 107 122 71 631 605 2973 1525
Fragments arranged by size:
2973 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2552 3324 4095 5251 1790
Size: 762 772 771 1156 2573
Fragments arranged by size:
2573 1156 772 771 762
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3765 4723 442
Size: 416 53 83 399 24 2348 958 1753
Fragments arranged by size:
2348 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2711 1056
Size: 1655 4379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2972 2392
Size: 580 5454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4694 1239
Size: 3455 2579
Bpu10I CC'TnA_GC
Cuts at: 2990 2990
Size: 6034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 5024 5322 4159
Size: 865 298 4871
Fragments arranged by size:
4871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2296 4697 882
Size: 617 797 2401 2219
Fragments arranged by size:
2401 2219 797 617
BsaAI yAC'GTr
Cuts at: 493 1204 3205 3825 493
Size: 711 2001 620 2702
Fragments arranged by size:
2702 2001 711 620
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4467 4865 5139 5286 5697 4467
Size: 398 274 147 411 4804
Fragments arranged by size:
4804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 2049 2079 2859
Size: 30 165 30 609 30 7 30 780
Cuts at: 2859 2889 1178
Size: 30 4323
Fragments arranged by size:
4323 780 609 165 30 30 30 30
30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 1186
Size: 834 5200
BseYI C'CCAG_C
Cuts at: 2318 2994 3329 5237 5733 2318
Size: 676 335 1908 496 2619
Fragments arranged by size:
2619 1908 676 496 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3299 3484 4245 5273 1540
Size: 275 186 1298 185 761 1028 2301
Fragments arranged by size:
2301 1298 1028 761 275 186 185
BsiHKAI G_wGCw'C
Cuts at: 3227 4095 4180 5251 3227
Size: 868 85 1071 4010
Fragments arranged by size:
4010 1071 868 85
BsmI GAATG_Cn'
Cuts at: 3545 3638 3545
Size: 93 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2552 2788 2796 3227 3324 3353 4095
Size: 399 762 236 8 431 97 29 742
Cuts at: 4095 4180 5251 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 762 742 431 399 236 97
85 29 8
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2825 3267 3296 3389 844
Size: 1981 442 29 93 3489
Fragments arranged by size:
3489 1981 442 93 29
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 3921 1394
Size: 429 1480 618 3507
Fragments arranged by size:
3507 1480 618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2847 4529 4703 65
Size: 1183 1599 1682 174 1396
Fragments arranged by size:
1682 1599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3063 4678 4865 1700
Size: 110 1253 1615 187 2869
Fragments arranged by size:
2869 1615 1253 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 3098 4094 5106 3098
Size: 996 1012 4026
Fragments arranged by size:
4026 1012 996
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2606 794
Size: 1812 4222
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 3043 1184
Size: 1859 4175
BstYI r'GATC_y
Cuts at: 1972 3364 3731 4116 4133 5585 5671 5683 6029
Size: 1392 367 385 17 1452 86 12 346
Cuts at: 6029 1972
Size: 1977
Fragments arranged by size:
1977 1452 1392 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2814 2814
Size: 6034
BtgI C'CryG_G
Cuts at: 513 1065 2004 2913 513
Size: 552 939 909 3634
Fragments arranged by size:
3634 939 909 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 2520 3082 3550 4344 4364 2147
Size: 373 562 468 794 20 3817
Fragments arranged by size:
3817 794 562 468 373 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2910 3296
Size: 54 1425 50 275 18 177 903 386
Cuts at: 3296 3481 4370 5999 8
Size: 185 889 1629 43
Fragments arranged by size:
1629 1425 903 889 386 275 185 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3296 3481 1537
Size: 275 1484 185 4090
Fragments arranged by size:
4090 1484 275 185
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3964 1353
Size: 1448 1163 3423
Fragments arranged by size:
3423 1448 1163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4621 4995 5141 387
Size: 1064 138 3032 374 146 1280
Fragments arranged by size:
3032 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3225 3225
Size: 6034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 4097 4694 5481 5749 1239
Size: 393 621 1844 597 787 268 1524
Fragments arranged by size:
1844 1524 787 621 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2549 2998 3320 3321 2549
Size: 449 322 1 5262
Fragments arranged by size:
5262 449 322 1
EcoRI G'AATT_C
Cuts at: 3343 3721 3343
Size: 378 5656
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 3077 5181 1678
Size: 1399 2104 2531
Fragments arranged by size:
2531 2104 1399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 2290 2322 4675
Size: 32 135 32 477 32 209 32 2353
Cuts at: 4675 4707 4749 4781 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2353 477 209 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3378 3624 669
Size: 988 281 1440 246 3079
Fragments arranged by size:
3079 1440 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2845 5148 5332 1305
Size: 1540 2303 184 2007
Fragments arranged by size:
2303 2007 1540 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4212
Fragments arranged by size:
4212 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3992 4351 4510 518
Size: 648 2826 359 159 2042
Fragments arranged by size:
2826 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2528 2680 2909 3265 3450 4127 5275
Size: 588 334 152 229 356 185 677 1148
Cuts at: 5275 5520 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 588 356 334 245 229
185 152
NaeI GCC'GGC
Cuts at: 3065 3065
Size: 6034
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4543
Fragments arranged by size:
4543 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NgoMIV G'CCGG_C
Cuts at: 3063 3063
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 2707 1052
Size: 1655 4379
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 2182 3392 4937 1132
Size: 97 953 1210 1545 2229
Fragments arranged by size:
2229 1545 1210 953 97
PasI CC'CwG_GG
Cuts at: 1348 2788 1348
Size: 1440 4594
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2859 3088 1108
Size: 1751 229 4054
Fragments arranged by size:
4054 1751 229
PfoI T'CCnGG_A
Cuts at: 2740 2740
Size: 6034
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 3604 3604
Size: 6034
PspOMI G'GGCC_C
Cuts at: 2548 3320 2548
Size: 772 5262
PstI C_TGCA'G
Cuts at: 830 2527 3248 3386 830
Size: 1697 721 138 3478
Fragments arranged by size:
3478 1697 721 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 2194 2528 2194
Size: 334 5700
SacI G_AGCT'C
Cuts at: 3227 3227
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SapI GCTCTTCn'nnn_
Cuts at: 2801 2801
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SfcI C'TryA_G
Cuts at: 826 1045 2523 3244 3382 4517 5198 5389 826
Size: 219 1478 721 138 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 721 681 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 3020 3054 3361 1872
Size: 1148 34 307 4545
Fragments arranged by size:
4545 1148 307 34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4138 5039 5301 820
Size: 197 926 2195 901 262 1553
Fragments arranged by size:
2195 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 3958 5
Size: 47 3906 2081
Fragments arranged by size:
3906 2081 47
StuI AGG'CCT
Cuts at: 1517 2595 1517
Size: 1078 4956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2760 3473 513
Size: 552 939 756 713 3074
Fragments arranged by size:
3074 939 756 713 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3031 3062 4061 4078 4231 4416 4861
Size: 1255 1451 31 999 17 153 185 445
Cuts at: 4861 325
Size: 1498
Fragments arranged by size:
1498 1451 1255 999 445 185 153 31
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4280 5992 96
Size: 274 80 33 51 1443 2303 1712 138
Fragments arranged by size:
2303 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2414 2446 5994 6026 2012
Size: 32 370 32 3548 32 2020
Fragments arranged by size:
3548 2020 370 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6034
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 2758
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 3018 3052 3359 1870
Size: 1148 34 307 4545
Fragments arranged by size:
4545 1148 307 34
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI