Restriction map for
pFN21AE6302
(Circular) (Six-base) MAPSORT of: pFN21AE6302.seq Check: 4664 from: 1 to: 5638
pFN21AE6302 5638 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2193 2628 2959 2193
Size: 435 331 4872
Fragments arranged by size:
4872 435 331
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2555 2591 2825 3701 5085 5353 1632
Size: 923 36 234 876 1384 268 1917
Fragments arranged by size:
1917 1384 923 876 268 234 36
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 5638
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2369 2778 4537 5514 1203
Size: 1166 409 1759 977 1327
Fragments arranged by size:
1759 1327 1166 977 409
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 1184
Size: 1170 4468
AlwNI CAG_nnn'CTG
Cuts at: 2564 2755 2803 3381 4953 2564
Size: 191 48 578 1572 3249
Fragments arranged by size:
3249 1572 578 191 48
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2963 1870
Size: 73 134 886 4545
Fragments arranged by size:
4545 886 134 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 2628 2959 4414 618
Size: 325 217 627 406 435 331 1455 1842
Fragments arranged by size:
1842 1455 627 435 406 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2582 2820 2957 1391
Size: 1191 238 137 4072
Fragments arranged by size:
4072 1191 238 137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 2671 2936 928
Size: 971 260 512 265 3630
Fragments arranged by size:
3630 971 512 265 260
BbvCI CC'TCA_GC
Cuts at: 2327 2327
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2210 2244 3829 3863 1903
Size: 34 50 34 189 34 1585 34 3678
Fragments arranged by size:
3678 1585 189 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2667 1548
Size: 1119 4519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2121 5633 2121
Size: 3512 2126
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2429 2840 3699 4855 1790
Size: 639 411 859 1156 2573
Fragments arranged by size:
2573 1156 859 639 411
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 3369 4327
Size: 416 53 83 399 24 1265 687 958
Cuts at: 4327 442
Size: 1753
Fragments arranged by size:
1753 1265 958 687 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2777 4298 1239
Size: 1538 1521 2579
Fragments arranged by size:
2579 1538 1521
Bpu10I CC'TnA_GC
Cuts at: 2264 2327 2264
Size: 63 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2804 3763 4628 4926 2804
Size: 959 865 298 3516
Fragments arranged by size:
3516 959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2429 4301 882
Size: 617 930 1872 2219
Fragments arranged by size:
2219 1872 930 617
BsaAI yAC'GTr
Cuts at: 493 1204 2149 2781 3429 493
Size: 711 945 632 648 2702
Fragments arranged by size:
2702 945 711 648 632
BsaBI GATnn'nnATC
Cuts at: 2126 5632 2126
Size: 3506 2132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 3445 3827 4462
Size: 53 83 186 936 708 1204 382 635
Cuts at: 4462 275
Size: 1451
Fragments arranged by size:
1451 1204 936 708 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2772 4071 4469 4743 4890 5301 2772
Size: 1299 398 274 147 411 3109
Fragments arranged by size:
3109 1299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 1178
Size: 30 165 30 1050 30 4333
Fragments arranged by size:
4333 1050 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2712 1186
Size: 1526 4112
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2301 3088 3849 4877 1540
Size: 275 186 39 261 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 261 186 39
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 2825 3149 3242 2825
Size: 324 93 5221
Fragments arranged by size:
5221 324 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2429 2582 2820 2840 2957 3699 3784
Size: 399 639 153 238 20 117 742 85
Cuts at: 3784 4855 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 639 399 238 153 117
85 20
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2607 3527 5257 2607
Size: 920 1730 2988
Fragments arranged by size:
2988 1730 920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 5537
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2121 2799 2968 3335 3720 3737 5189 5275
Size: 149 678 169 367 385 17 1452 86
Cuts at: 5275 5287 5633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 678 385 367 346 169 149
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2189 2236 2257 2412 2430 2500 513
Size: 552 1124 47 21 155 18 70 3651
Fragments arranged by size:
3651 1124 552 155 70 47 21 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2298 2403
Size: 54 1425 50 275 18 207 261 105
Cuts at: 2403 2874 3085 3974 5603 8
Size: 471 211 889 1629 43
Fragments arranged by size:
1629 1425 889 471 275 261 211 207
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2298 3085 1537
Size: 275 225 261 787 4090
Fragments arranged by size:
4090 787 275 261 225
EarI CTCTTCn'nnn_
Cuts at: 1353 3568 1353
Size: 2215 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2555 2591 2777 2825 3701 4298 5085
Size: 393 923 36 186 48 876 597 787
Cuts at: 5085 5353 1239
Size: 268 1524
Fragments arranged by size:
1524 923 876 787 597 393 268 186
48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2807 2573
Size: 234 5404
EcoO109I rG'GnC_Cy
Cuts at: 2551 2752 2551
Size: 201 5437
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 2621 4144 2621
Size: 1523 4115
HaeII r_GCGC'y
Cuts at: 1678 2179 2397 4785 1678
Size: 501 218 2388 2531
Fragments arranged by size:
2531 2388 501 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2453 2485 4279
Size: 32 135 32 590 32 259 32 1794
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1794 590 259 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2197 2632 2963 2197
Size: 435 331 4872
Fragments arranged by size:
4872 435 331
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 2369 5514 2369
Size: 3145 2493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2876 10
Size: 54 1425 343 1044 2772
Fragments arranged by size:
2772 1425 1044 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3596 3955 4114 518
Size: 648 1278 1152 359 159 2042
Fragments arranged by size:
2042 1278 1152 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2167 2191 2259 3054 3731 4879 5124
Size: 555 6 24 68 795 677 1148 245
Cuts at: 5124 1606
Size: 2120
Fragments arranged by size:
2120 1148 795 677 555 245 68 24
6
NcoI C'CATG_G
Cuts at: 513 1065 2236 2430 513
Size: 552 1171 194 3721
Fragments arranged by size:
3721 1171 552 194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 2037 3085 2037
Size: 1048 4590
NspI r_CATG'y
Cuts at: 1132 1229 2341 2996 4541 1132
Size: 97 1112 655 1545 2229
Fragments arranged by size:
2229 1545 1112 655 97
PasI CC'CwG_GG
Cuts at: 1348 2350 1348
Size: 1002 4636
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PfoI T'CCnGG_A
Cuts at: 2054 2054
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PmlI CAC'GTG
Cuts at: 2781 2781
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2166 2990 830
Size: 1336 824 3478
Fragments arranged by size:
3478 1336 824
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2161 2167 2161
Size: 6 5632
SacII CC_GC'GG
Cuts at: 2192 2260 2192
Size: 68 5570
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2162 2986 4121 4802 4993 826
Size: 219 1117 824 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 824 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2079 2965 1872
Size: 207 886 4545
Fragments arranged by size:
4545 886 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 3742 4643 4905 820
Size: 197 926 876 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 876 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2236 2292 2430 3077 513
Size: 552 1171 56 138 647 3074
Fragments arranged by size:
3074 1171 647 552 138 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3884 5596
Size: 274 80 33 51 1443 507 1400 1712
Cuts at: 5596 96
Size: 138
Fragments arranged by size:
1712 1443 1400 507 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2077 2963 1870
Size: 207 886 4545
Fragments arranged by size:
4545 886 207
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI