Restriction map for
pFN21AE6294
(Circular) (Six-base) MAPSORT of: pFN21AE6294.seq Check: 7367 from: 1 to: 5353
pFN21AE6294 5353 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3163 278
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2696 1937
Size: 759 4594
Acc65I G'GTAC_C
Cuts at: 2674 2674
Size: 5353
AclI AA'CG_TT
Cuts at: 1563 3480 3853 1563
Size: 1917 373 3063
Fragments arranged by size:
3063 1917 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2557 3416 4800 5068 1632
Size: 925 859 1384 268 1917
Fragments arranged by size:
1917 1384 925 859 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5156
AflIII A'CryG_T
Cuts at: 1203 4252 5229 1203
Size: 3049 977 1327
Fragments arranged by size:
3049 1327 977
AgeI A'CCGG_T
Cuts at: 2279 4184 2279
Size: 1905 3448
AhdI GACnn_n'nnGTC
Cuts at: 4082 4082
Size: 5353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5353
AlwNI CAG_nnn'CTG
Cuts at: 2194 3096 4668 2194
Size: 902 1572 2879
Fragments arranged by size:
2879 1572 902
ApaLI G'TGCA_C
Cuts at: 2078 3410 4566 2078
Size: 1332 1156 2865
Fragments arranged by size:
2865 1332 1156
ApoI r'AATT_y
Cuts at: 1493 2662 2888 3040 5149 1493
Size: 1169 226 152 2109 1697
Fragments arranged by size:
2109 1697 1169 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3907 160
Size: 872 2875 1606
Fragments arranged by size:
2875 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2678 1870
Size: 73 574 161 4545
Fragments arranged by size:
4545 574 161 73
BamHI G'GATC_C
Cuts at: 2683 3050 2683
Size: 367 4986
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2674 4129 618
Size: 325 217 627 887 1455 1842
Fragments arranged by size:
1842 1455 887 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2107 2201 2626 2672 1391
Size: 716 94 425 46 4072
Fragments arranged by size:
4072 716 425 94 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 2588 928
Size: 971 664 25 3693
Fragments arranged by size:
3693 971 664 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3544 3578 1903
Size: 34 1607 34 3678
Fragments arranged by size:
3678 1607 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3245 4455 3245
Size: 1210 4143
BclI T'GATC_A
Cuts at: 1548 2323 1548
Size: 775 4578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3964 136
Size: 107 122 71 631 605 2292 1525
Fragments arranged by size:
2292 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5348 5348
Size: 5353
BlpI GC'TnA_GC
Cuts at: 2770 2770
Size: 5353
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2157 3414 4570 1790
Size: 292 75 1257 1156 2573
Fragments arranged by size:
2573 1257 1156 292 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3084 4042 442
Size: 416 53 83 399 24 1667 958 1753
Fragments arranged by size:
1753 1667 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 2179 2363 4013 1239
Size: 882 58 184 1650 2579
Fragments arranged by size:
2579 1650 882 184 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3478 4343 4641 3478
Size: 865 298 4190
Fragments arranged by size:
4190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4016 882
Size: 617 2517 2219
Fragments arranged by size:
2517 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2083 3144 493
Size: 711 879 1061 2702
Fragments arranged by size:
2702 1061 879 711
BsaBI GATnn'nnATC
Cuts at: 2453 5347 2453
Size: 2894 2459
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3160 3542 4177 275
Size: 53 83 186 936 1627 382 635 1451
Fragments arranged by size:
1627 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2170 2279 3786 4184 4458 4605 5016 2170
Size: 109 1507 398 274 147 411 2507
Fragments arranged by size:
2507 1507 411 398 274 147 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 1178
Size: 30 165 30 664 30 4434
Fragments arranged by size:
4434 664 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5353
BseYI C'CCAG_C
Cuts at: 2143 2182 2419 4556 5052 2143
Size: 39 237 2137 496 2444
Fragments arranged by size:
2444 2137 496 237 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4780
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3564 4592 1540
Size: 275 186 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 3414 3499 4570 2082
Size: 1332 85 1071 2865
Fragments arranged by size:
2865 1332 1071 85
BsmI GAATG_Cn'
Cuts at: 2864 2957 2864
Size: 93 5260
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2107 2157 2201 2626 2672 3414
Size: 399 292 25 50 44 425 46 742
Cuts at: 3414 3499 4570 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 425 399 292 85 50
46 44 25
BspEI T'CCGG_A
Cuts at: 5016 5016
Size: 5353
BspHI T'CATG_A
Cuts at: 3242 4972 3242
Size: 1730 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2532 2708 844
Size: 1688 176 3489
Fragments arranged by size:
3489 1688 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3240 1394
Size: 429 1417 3507
Fragments arranged by size:
3507 1417 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 3848 4022 65
Size: 1183 1299 1301 174 1396
Fragments arranged by size:
1396 1301 1299 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 3997 4184 1700
Size: 110 469 1718 187 2869
Fragments arranged by size:
2869 1718 469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5353
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5353
BssSI C'ACGA_G
Cuts at: 2459 3413 4425 2459
Size: 954 1012 3387
Fragments arranged by size:
3387 1012 954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5353
BstBI TT'CG_AA
Cuts at: 4157 4157
Size: 5353
BstEII G'GTnAC_C
Cuts at: 1184 2275 1184
Size: 1091 4262
BstYI r'GATC_y
Cuts at: 1972 2683 3050 3435 3452 4904 4990 5002 5348
Size: 711 367 385 17 1452 86 12 346
Cuts at: 5348 1972
Size: 1977
Fragments arranged by size:
1977 1452 711 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2616 513
Size: 552 939 66 546 3250
Fragments arranged by size:
3250 939 552 546 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4614
Fragments arranged by size:
4614 683 56
BtsI GCAGTG_nn'
Cuts at: 2423 2869 3663 3683 2423
Size: 446 794 20 4093
Fragments arranged by size:
4093 794 446 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5318
DraI TTT'AAA
Cuts at: 2657 2657
Size: 5353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4360 809
Size: 507 3044 1802
Fragments arranged by size:
3044 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2800 3689
Size: 54 1425 50 275 18 411 559 889
Cuts at: 3689 5318 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 559 411 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2800 1537
Size: 275 988 4090
Fragments arranged by size:
4090 988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2396 3283 1353
Size: 884 159 887 3423
Fragments arranged by size:
3423 887 884 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3940 4314 4460 387
Size: 1064 138 2351 374 146 1280
Fragments arranged by size:
2351 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2179 2363 2557 3416 4013 4800
Size: 393 489 58 184 194 859 597 787
Cuts at: 4800 5068 1239
Size: 268 1524
Fragments arranged by size:
1524 859 787 597 489 393 268 194
184 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 5353
EcoO109I rG'GnC_Cy
Cuts at: 2521 2521
Size: 5353
EcoRI G'AATT_C
Cuts at: 2662 3040 2662
Size: 378 4975
FspI TGC'GCA
Cuts at: 3859 3859
Size: 5353
HaeII r_GCGC'y
Cuts at: 1678 2444 4500 1678
Size: 766 2056 2531
Fragments arranged by size:
2531 2056 766
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2490 2522 3994 4026 4068
Size: 32 135 32 918 32 1472 32 42
Cuts at: 4068 4100 1373
Size: 32 2626
Fragments arranged by size:
2626 1472 918 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2044 2697 2943 669
Size: 988 281 106 653 246 3079
Fragments arranged by size:
3079 988 653 281 246 106
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5353
HpaI GTT'AAC
Cuts at: 2943 2943
Size: 5353
KpnI G_GTAC'C
Cuts at: 2678 2678
Size: 5353
MfeI C'AATT_G
Cuts at: 2952 2952
Size: 5353
MluI A'CGCG_T
Cuts at: 5229 5229
Size: 5353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4467 4651 1305
Size: 3162 184 2007
Fragments arranged by size:
3162 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3531
Fragments arranged by size:
3531 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2459 2474 3311 3670 3829 518
Size: 648 909 384 15 837 359 159 2042
Fragments arranged by size:
2042 909 837 648 384 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2120 2269 2297 2383 2769 3446 4594 4839
Size: 514 149 28 86 386 677 1148 245
Cuts at: 4839 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 514 386 245 149 86
28
NcoI C'CATG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 3796
Fragments arranged by size:
3796 939 552 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5353
NotI GC'GGCC_GC
Cuts at: 2800 2800
Size: 5353
NspI r_CATG'y
Cuts at: 1132 1229 2711 4256 1132
Size: 97 1482 1545 2229
Fragments arranged by size:
2229 1545 1482 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5353
PciI A'CATG_T
Cuts at: 4252 4252
Size: 5353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5353
PmeI GTTT'AAAC
Cuts at: 2657 2657
Size: 5353
PmlI CAC'GTG
Cuts at: 2083 2083
Size: 5353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 4935 4967 3422
Size: 32 1481 32 3808
Fragments arranged by size:
3808 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2521
Size: 5353
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5353
PsiI TTA'TAA
Cuts at: 2923 2923
Size: 5353
PstI C_TGCA'G
Cuts at: 830 2705 830
Size: 1875 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5353
PvuII CAG'CTG
Cuts at: 2120 2269 2297 2383 2120
Size: 149 28 86 5090
Fragments arranged by size:
5090 149 86 28
SalI G'TCGA_C
Cuts at: 1936 2695 1936
Size: 759 4594
SbfI CC_TGCA'GG
Cuts at: 2705 2705
Size: 5353
ScaI AGT'ACT
Cuts at: 3601 5313 3601
Size: 1712 3641
SexAI A'CCwGG_T
Cuts at: 2590 2590
Size: 5353
SfcI C'TryA_G
Cuts at: 826 1045 2224 2292 2701 3836 4517 4708 826
Size: 219 1179 68 409 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 681 409 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5353
SmaI CCC'GGG
Cuts at: 1872 2519 2680 1872
Size: 647 161 4545
Fragments arranged by size:
4545 647 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3457 4358 4620 820
Size: 197 926 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3144 493
Size: 2651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5353
SphI G_CATG'C
Cuts at: 1132 2711 1132
Size: 1579 3774
SspI AAT'ATT
Cuts at: 5 52 3277 5
Size: 47 3225 2081
Fragments arranged by size:
3225 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5353
StyI C'CwwG_G
Cuts at: 513 1065 2004 2035 2070 2482 2792 513
Size: 552 939 31 35 412 310 3074
Fragments arranged by size:
3074 939 552 412 310 35 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3380 3397 3550 3735 4180 325
Size: 1255 1800 17 153 185 445 1498
Fragments arranged by size:
1800 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3599 5311 96
Size: 274 80 33 51 1443 1622 1712 138
Fragments arranged by size:
1712 1622 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2302 2334 5313 5345 2067
Size: 32 203 32 2979 32 2075
Fragments arranged by size:
2979 2075 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5353
XbaI T'CTAG_A
Cuts at: 2689 2689
Size: 5353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2258 2258
Size: 5353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5353
XmaI C'CCGG_G
Cuts at: 1870 2517 2678 1870
Size: 647 161 4545
Fragments arranged by size:
4545 647 161
XmnI GAAnn'nnTTC
Cuts at: 3482 3482
Size: 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3161 276
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI