Restriction map for
pFN21AE6288
(Circular) (Six-base) MAPSORT of: pFN21AE6288.seq Check: 7085 from: 1 to: 5626
pFN21AE6288 5626 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2436 2550 2692 2436
Size: 114 142 5370
Fragments arranged by size:
5370 142 114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 3689 5073 5341 1632
Size: 731 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 731 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2019 4525 5502 1203
Size: 816 2506 977 1327
Fragments arranged by size:
2506 1327 977 816
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 2477 1166
Size: 1311 4315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2495 1184
Size: 1311 4315
AlwNI CAG_nnn'CTG
Cuts at: 2362 3369 4941 2362
Size: 1007 1572 3047
Fragments arranged by size:
3047 1572 1007
ApaI G_GGCC'C
Cuts at: 2190 2574 2619 2720 2190
Size: 384 45 101 5096
Fragments arranged by size:
5096 384 101 45
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2144 2183 2674 2823 2951 1870
Size: 73 175 26 39 491 149 128 4545
Fragments arranged by size:
4545 491 175 149 128 73 39 26
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2947 4402 618
Size: 325 217 627 312 848 1455 1842
Fragments arranged by size:
1842 1455 848 627 325 312 217
BanII G_rGCy'C
Cuts at: 1391 2184 2190 2415 2574 2619 2720 2945 1391
Size: 793 6 225 159 45 101 225 4072
Fragments arranged by size:
4072 793 225 225 159 101 45 6
BbeI G_GCGC'C
Cuts at: 2103 2103
Size: 5626
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2104 928
Size: 971 205 4450
Fragments arranged by size:
4450 971 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2081 2123
Size: 107 122 71 631 605 400 9 42
Cuts at: 2123 2609 4237 136
Size: 486 1628 1525
Fragments arranged by size:
1628 1525 631 605 486 400 122 107
71 42 9
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2484 2574 2619 2720 3687 4843 1790
Size: 400 294 90 45 101 967 1156 2573
Fragments arranged by size:
2573 1156 967 400 294 101 90 45
BmgBI CAC'GTC
Cuts at: 1318 2625 1318
Size: 1307 4319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2087 3357 4315
Size: 416 53 83 399 24 670 1270 958
Cuts at: 4315 442
Size: 1753
Fragments arranged by size:
1753 1270 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2711 4286 1239
Size: 941 531 1575 2579
Fragments arranged by size:
2579 1575 941 531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2698 3417 493
Size: 711 1494 719 2702
Fragments arranged by size:
2702 1494 719 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 3433 3815 4450
Size: 53 83 186 936 567 1333 382 635
Cuts at: 4450 275
Size: 1451
Fragments arranged by size:
1451 1333 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5401
Fragments arranged by size:
5401 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4479
BseYI C'CCAG_C
Cuts at: 2195 2362 2400 2713 4829 5325 2195
Size: 167 38 313 2116 496 2496
Fragments arranged by size:
2496 2116 496 313 167 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2365 2408 2522 2720 2735 1163
Size: 573 423 206 43 114 198 15 4054
Fragments arranged by size:
4054 573 423 206 198 114 43 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2613 3076 3837 4865 1540
Size: 275 186 612 463 761 1028 2301
Fragments arranged by size:
2301 1028 761 612 463 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2415 2821 3687 3772 4843 2184
Size: 231 406 866 85 1071 2967
Fragments arranged by size:
2967 1071 866 406 231 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2190 2415 2484 2574 2619 2720
Size: 399 394 6 225 69 90 45 101
Cuts at: 2720 2821 2945 3687 3772 4843 1391
Size: 101 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 225 124 101
101 90 85 69 45 6
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2223 2430 2436 2550 2692 2981 844
Size: 1156 223 207 6 114 142 289 3489
Fragments arranged by size:
3489 1156 289 223 207 142 114 6
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2833 4270 4457 1700
Size: 110 1023 1437 187 2869
Fragments arranged by size:
2869 1437 1023 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 794
Size: 1637 3989
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 2257 2590 1184
Size: 1073 333 4220
Fragments arranged by size:
4220 1073 333
BstYI r'GATC_y
Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621
Size: 984 367 385 17 1452 86 12 346
Cuts at: 5621 1972
Size: 1977
Fragments arranged by size:
1977 1452 984 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2845 2845
Size: 5626
BtgI C'CryG_G
Cuts at: 513 1065 2004 2485 513
Size: 552 939 481 3654
Fragments arranged by size:
3654 939 552 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 2905 3142 3936 3956 2905
Size: 237 794 20 4575
Fragments arranged by size:
4575 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2313 2610
Size: 54 1425 50 275 18 447 36 297
Cuts at: 2610 2649 2797 3073 3962 5591 8
Size: 39 148 276 889 1629 43
Fragments arranged by size:
1629 1425 889 447 297 276 275 148
54 50 43 39 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2610 3073 1537
Size: 275 798 463 4090
Fragments arranged by size:
4090 798 463 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2246 3556 1353
Size: 884 9 1310 3423
Fragments arranged by size:
3423 1310 884 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 2413 2182
Size: 231 5395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2363 2711 3689 4286 5073 5341
Size: 393 548 183 348 978 597 787 268
Cuts at: 5341 1239
Size: 1524
Fragments arranged by size:
1524 978 787 597 548 393 348 268
183
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2724 2057
Size: 667 4959
EcoO109I rG'GnC_Cy
Cuts at: 2083 2148 2187 2271 2616 2717 2083
Size: 65 39 84 345 101 4992
Fragments arranged by size:
4992 345 101 84 65 39
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FspI TGC'GCA
Cuts at: 2471 4132 2471
Size: 1661 3965
HaeII r_GCGC'y
Cuts at: 1678 2103 2495 4773 1678
Size: 425 392 2278 2531
Fragments arranged by size:
2531 2278 425 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2258 2290 4267 4299 4341
Size: 32 135 32 686 32 1977 32 42
Cuts at: 4341 4373 1373
Size: 32 2626
Fragments arranged by size:
2626 1977 686 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KasI G'GCGC_C
Cuts at: 2099 2099
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 3584 3943 4102 518
Size: 648 1311 1107 359 159 2042
Fragments arranged by size:
2042 1311 1107 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2568 2576 3042 3719 4867 5112 1606
Size: 962 8 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 962 677 466 245 8
NaeI GCC'GGC
Cuts at: 2835 2835
Size: 5626
NarI GG'CG_CC
Cuts at: 2100 2100
Size: 5626
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NgoMIV G'CCGG_C
Cuts at: 2833 2833
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2371 2984 4529 1132
Size: 97 1142 613 1545 2229
Fragments arranged by size:
2229 1545 1142 613 97
PasI CC'CwG_GG
Cuts at: 1348 2190 1348
Size: 842 4784
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PfoI T'CCnGG_A
Cuts at: 2157 2883 2157
Size: 726 4900
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2174 1613
Size: 561 5065
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PspOMI G'GGCC_C
Cuts at: 2186 2570 2615 2716 2186
Size: 384 45 101 5096
Fragments arranged by size:
5096 384 101 45
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 5594
PstI C_TGCA'G
Cuts at: 830 2301 2436 2459 2978 830
Size: 1471 135 23 519 3478
Fragments arranged by size:
3478 1471 519 135 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SacI G_AGCT'C
Cuts at: 2184 2415 2184
Size: 231 5395
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2246 2237
Size: 9 5617
SbfI CC_TGCA'GG
Cuts at: 2301 2978 2301
Size: 677 4949
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2297 2432 2455 2563 2974 4109 4790
Size: 219 1252 135 23 108 411 1135 681
Cuts at: 4790 4981 826
Size: 191 1471
Fragments arranged by size:
1471 1252 1135 681 411 219 191 135
108 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 2081 2609 2081
Size: 528 5098
SfoI GGC'GCC
Cuts at: 2101 2101
Size: 5626
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2120 2953 1872
Size: 248 833 4545
Fragments arranged by size:
4545 833 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2371 2984 1132
Size: 1239 613 3774
Fragments arranged by size:
3774 1239 613
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2262 3065 513
Size: 552 939 75 183 803 3074
Fragments arranged by size:
3074 939 803 552 183 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2870 3653 3670 3823 4008 4453 325
Size: 1255 1290 783 17 153 185 445 1498
Fragments arranged by size:
1498 1290 1255 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 3872 5584
Size: 274 80 33 51 1443 729 1166 1712
Cuts at: 5584 96
Size: 138
Fragments arranged by size:
1712 1443 1166 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2118 2951 1870
Size: 248 833 4545
Fragments arranged by size:
4545 833 248
XmnI GAAnn'nnTTC
Cuts at: 2789 3755 2789
Size: 966 4660
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PvuII RsrII SacII SanDI SexAI SrfI SwaI XcmI