Restriction map for
pFN21AE6287
(Circular) (Six-base) MAPSORT of: pFN21AE6287.seq Check: 1202 from: 1 to: 5593
pFN21AE6287 5593 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2936 1937
Size: 999 4594
Acc65I G'GTAC_C
Cuts at: 2914 2914
Size: 5593
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2572 2874 3656 5040 5308 1632
Size: 940 302 782 1384 268 1917
Fragments arranged by size:
1917 1384 940 782 302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 2601 4492 5469 1203
Size: 1398 1891 977 1327
Fragments arranged by size:
1891 1398 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 2356 2710 3336 4908 2356
Size: 354 626 1572 3041
Fragments arranged by size:
3041 1572 626 354
ApaI G_GGCC'C
Cuts at: 2311 2311
Size: 5593
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2902 3128 3280 5389 1493
Size: 1409 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2918 1870
Size: 73 130 845 4545
Fragments arranged by size:
4545 845 130 73
BamHI G'GATC_C
Cuts at: 2923 3290 2923
Size: 367 5226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2914 4369 618
Size: 325 217 627 1127 1455 1842
Fragments arranged by size:
1842 1455 1127 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 2311 2912 1391
Size: 766 154 601 4072
Fragments arranged by size:
4072 766 601 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2578 928
Size: 971 679 3943
Fragments arranged by size:
3943 971 679
BbvCI CC'TCA_GC
Cuts at: 2645 2645
Size: 5593
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2092 2126 3784 3818 1903
Size: 34 155 34 1658 34 3678
Fragments arranged by size:
3678 1658 155 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3485 4695 3485
Size: 1210 4383
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BfrBI ATG'CAT
Cuts at: 2573 2573
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5588 5588
Size: 5593
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2311 3654 4810 1790
Size: 226 295 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 295 226
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2438 3324 4282
Size: 416 53 83 399 24 1021 886 958
Cuts at: 4282 442
Size: 1753
Fragments arranged by size:
1753 1021 958 886 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4253 1239
Size: 3014 2579
Bpu10I CC'TnA_GC
Cuts at: 2645 2645
Size: 5593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2862 3718 4583 4881 2862
Size: 856 865 298 3574
Fragments arranged by size:
3574 865 856 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4256 882
Size: 617 2757 2219
Fragments arranged by size:
2757 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 5587 5587
Size: 5593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3400 3782 4417 275
Size: 53 83 186 936 1867 382 635 1451
Fragments arranged by size:
1867 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4026 4424 4698 4845 5256 4026
Size: 398 274 147 411 4363
Fragments arranged by size:
4363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5368
Fragments arranged by size:
5368 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2263 1186
Size: 1077 4516
BseYI C'CCAG_C
Cuts at: 2176 4796 5292 2176
Size: 2620 496 2477
Fragments arranged by size:
2620 2477 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2844 1163
Size: 573 1108 3912
Fragments arranged by size:
3912 1108 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 3043 3804 4832 1540
Size: 275 186 105 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 2061 3654 3739 4810 2061
Size: 1593 85 1071 2844
Fragments arranged by size:
2844 1593 1071 85
BsmI GAATG_Cn'
Cuts at: 2093 3104 3197 2093
Size: 1011 93 4489
Fragments arranged by size:
4489 1011 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2231 2231
Size: 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2061 2157 2311 2912 3654 3739
Size: 399 226 45 96 154 601 742 85
Cuts at: 3739 4810 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 601 399 226 154 96
85 45
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 3482 5212 3482
Size: 1730 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2948 844
Size: 1337 767 3489
Fragments arranged by size:
3489 1337 767
BsrBI CCG'CTC
Cuts at: 1394 1823 2072 3480 1394
Size: 429 249 1408 3507
Fragments arranged by size:
3507 1408 429 249
BsrDI GCAATG_nn'
Cuts at: 65 1248 2688 4088 4262 65
Size: 1183 1440 1400 174 1396
Fragments arranged by size:
1440 1400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4237 4424 1700
Size: 110 2427 187 2869
Fragments arranged by size:
2869 2427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 3653 4665 3653
Size: 1012 4581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 3677
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2860 2923 3290 3675 3692 5144 5230 5242
Size: 888 63 367 385 17 1452 86 12
Cuts at: 5242 5588 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 888 385 367 346 86 63
17 12
Bsu36I CC'TnA_GG
Cuts at: 2141 2141
Size: 5593
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4102
Fragments arranged by size:
4102 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2782 487
Size: 683 56 1556 3298
Fragments arranged by size:
3298 1556 683 56
BtsI GCAGTG_nn'
Cuts at: 2535 2699 3109 3903 3923 2535
Size: 164 410 794 20 4205
Fragments arranged by size:
4205 794 410 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2814 3040
Size: 54 1425 50 275 18 219 765 226
Cuts at: 3040 3929 5558 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 765 275 226 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 1537
Size: 275 1228 4090
Fragments arranged by size:
4090 1228 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 3523 1353
Size: 936 1234 3423
Fragments arranged by size:
3423 1234 936
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4180 4554 4700 387
Size: 1064 138 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2572 2874 3656 4253 5040 5308 1239
Size: 393 940 302 782 597 787 268 1524
Fragments arranged by size:
1524 940 787 782 597 393 302 268
EcoO109I rG'GnC_Cy
Cuts at: 2308 2353 2308
Size: 45 5548
EcoRI G'AATT_C
Cuts at: 2902 3280 2902
Size: 378 5215
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 4740 1678
Size: 3062 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2362 2394 2485 2517 2852
Size: 32 135 32 790 32 91 32 335
Cuts at: 2852 2884 4234 4266 4308 4340 1373
Size: 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 790 335 135 91 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2478 2937 3183 669
Size: 988 281 540 459 246 3079
Fragments arranged by size:
3079 988 540 459 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5593
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2918 2918
Size: 5593
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4707 4891 1305
Size: 3402 184 2007
Fragments arranged by size:
3402 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2816 10
Size: 54 1425 343 984 2787
Fragments arranged by size:
2787 1425 984 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2561 2600 2801 3551 3910 4069
Size: 648 843 552 39 201 750 359 159
Cuts at: 4069 518
Size: 2042
Fragments arranged by size:
2042 843 750 648 552 359 201 159
39
MspA1I CmG'CkG
Cuts at: 1606 3009 3686 4834 5079 1606
Size: 1403 677 1148 245 2120
Fragments arranged by size:
2120 1403 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4102
Fragments arranged by size:
4102 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5593
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 3040 3040
Size: 5593
NruI TCG'CGA
Cuts at: 2085 2085
Size: 5593
NsiI A_TGCA'T
Cuts at: 2575 2575
Size: 5593
NspI r_CATG'y
Cuts at: 1132 1229 2605 2951 4496 1132
Size: 97 1376 346 1545 2229
Fragments arranged by size:
2229 1545 1376 346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 2601 4492 2601
Size: 1891 3702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PspOMI G'GGCC_C
Cuts at: 2307 2307
Size: 5593
PstI C_TGCA'G
Cuts at: 830 2530 2706 2945 830
Size: 1700 176 239 3478
Fragments arranged by size:
3478 1700 239 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 5593
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 2244 3841 5553 2244
Size: 1597 1712 2284
Fragments arranged by size:
2284 1712 1597
SfcI C'TryA_G
Cuts at: 826 1045 2526 2702 2941 4076 4757 4948 826
Size: 219 1481 176 239 1135 681 191 1471
Fragments arranged by size:
1481 1471 1135 681 239 219 191 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2920 1872
Size: 1048 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2877 3697 4598 4860 820
Size: 197 926 934 820 901 262 1553
Fragments arranged by size:
1553 934 926 901 820 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SspI AAT'ATT
Cuts at: 5 52 3517 5
Size: 47 3465 2081
Fragments arranged by size:
3465 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 2004 2743 3032 513
Size: 552 939 739 289 3074
Fragments arranged by size:
3074 939 739 552 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2420 2551 3620 3637 3790 3975
Size: 1255 633 207 131 1069 17 153 185
Cuts at: 3975 4420 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1069 633 445 207 185 153
131 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2242 3839 5551
Size: 274 80 33 51 1443 265 1597 1712
Cuts at: 5551 96
Size: 138
Fragments arranged by size:
1712 1597 1443 274 265 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5553 5585 5553
Size: 32 5561
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5593
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2918 1870
Size: 1048 4545
XmnI GAAnn'nnTTC
Cuts at: 2255 3722 2255
Size: 1467 4126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI
PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI