Restriction map for
pFN21AE6285
(Circular) (Six-base) MAPSORT of: pFN21AE6285.seq Check: 3369 from: 1 to: 5542
pFN21AE6285 5542 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 278
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 1937
Size: 948 4594
Acc65I G'GTAC_C
Cuts at: 2863 2863
Size: 5542
AclI AA'CG_TT
Cuts at: 1563 3669 4042 1563
Size: 2106 373 3063
Fragments arranged by size:
3063 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2574 3605 4989 5257 1632
Size: 542 400 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 542 400 268
AflII C'TTAA_G
Cuts at: 820 1017 2333 820
Size: 197 1316 4029
Fragments arranged by size:
4029 1316 197
AflIII A'CryG_T
Cuts at: 1203 4441 5418 1203
Size: 3238 977 1327
Fragments arranged by size:
3238 1327 977
AgeI A'CCGG_T
Cuts at: 4373 4373
Size: 5542
AhdI GACnn_n'nnGTC
Cuts at: 4271 4271
Size: 5542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5542
AlwNI CAG_nnn'CTG
Cuts at: 2041 3285 4857 2041
Size: 1244 1572 2726
Fragments arranged by size:
2726 1572 1244
ApaLI G'TGCA_C
Cuts at: 3599 4755 3599
Size: 1156 4386
ApoI r'AATT_y
Cuts at: 1493 2084 2851 3077 3229 5338 1493
Size: 591 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 591 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4096 160
Size: 872 3064 1606
Fragments arranged by size:
3064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 1870
Size: 73 924 4545
Fragments arranged by size:
4545 924 73
BamHI G'GATC_C
Cuts at: 2872 3239 2872
Size: 367 5175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2863 4318 618
Size: 325 217 627 1076 1455 1842
Fragments arranged by size:
1842 1455 1076 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2861 1391
Size: 1470 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2334 928
Size: 971 435 4136
Fragments arranged by size:
4136 971 435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3733 3767 1903
Size: 34 1796 34 3678
Fragments arranged by size:
3678 1796 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3434 4644 3434
Size: 1210 4332
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4153 136
Size: 107 122 71 631 605 2481 1525
Fragments arranged by size:
2481 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5537 5537
Size: 5542
BlpI GC'TnA_GC
Cuts at: 2959 2959
Size: 5542
Bme1580I G_kGCm'C
Cuts at: 1790 3603 4759 1790
Size: 1813 1156 2573
Fragments arranged by size:
2573 1813 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2739 3273 4231
Size: 416 53 83 399 24 1322 534 958
Cuts at: 4231 442
Size: 1753
Fragments arranged by size:
1753 1322 958 534 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4202 1239
Size: 2963 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2484 3667 4532 4830 2484
Size: 1183 865 298 3196
Fragments arranged by size:
3196 1183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4205 882
Size: 617 2706 2219
Fragments arranged by size:
2706 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2837 3333 493
Size: 711 1633 496 2702
Fragments arranged by size:
2702 1633 711 496
BsaBI GATnn'nnATC
Cuts at: 5536 5536
Size: 5542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3349 3731 4366 275
Size: 53 83 186 936 1816 382 635 1451
Fragments arranged by size:
1816 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2078 3975 4373 4647 4794 5205 2078
Size: 1897 398 274 147 411 2415
Fragments arranged by size:
2415 1897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5317
Fragments arranged by size:
5317 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5542
BseYI C'CCAG_C
Cuts at: 2109 4745 5241 2109
Size: 2636 496 2410
Fragments arranged by size:
2636 2410 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 2859 1163
Size: 573 406 717 3846
Fragments arranged by size:
3846 717 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2992 3753 4781 1540
Size: 275 186 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2401 3603 3688 4759 2401
Size: 1202 85 1071 3184
Fragments arranged by size:
3184 1202 1071 85
BsmI GAATG_Cn'
Cuts at: 3053 3146 3053
Size: 93 5449
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2401 2861 3603 3688 4759 1391
Size: 399 611 460 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 460 399 85
BspEI T'CCGG_A
Cuts at: 5205 5205
Size: 5542
BspHI T'CATG_A
Cuts at: 3431 5161 3431
Size: 1730 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 844
Size: 2053 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3429 1394
Size: 429 1606 3507
Fragments arranged by size:
3507 1606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4037 4211 65
Size: 1183 2789 174 1396
Fragments arranged by size:
2789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4186 4373 1700
Size: 110 2376 187 2869
Fragments arranged by size:
2869 2376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5542
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5542
BssSI C'ACGA_G
Cuts at: 3602 4614 3602
Size: 1012 4530
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5542
BstBI TT'CG_AA
Cuts at: 4346 4346
Size: 5542
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5542
BstYI r'GATC_y
Cuts at: 1972 2872 3239 3624 3641 5093 5179 5191 5537
Size: 900 367 385 17 1452 86 12 346
Cuts at: 5537 1972
Size: 1977
Fragments arranged by size:
1977 1452 900 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4990
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4803
Fragments arranged by size:
4803 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 3058 3852 3872 2473
Size: 585 794 20 4143
Fragments arranged by size:
4143 794 585 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5507
DraI TTT'AAA
Cuts at: 2846 2846
Size: 5542
DraIII CAC_nnn'GTG
Cuts at: 2178 2178
Size: 5542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4549 809
Size: 507 3233 1802
Fragments arranged by size:
3233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2989 3878
Size: 54 1425 50 275 18 213 946 889
Cuts at: 3878 5507 8
Size: 1629 43
Fragments arranged by size:
1629 1425 946 889 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2989 1537
Size: 275 1177 4090
Fragments arranged by size:
4090 1177 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2132 2168 3472 1353
Size: 710 69 36 1304 3423
Fragments arranged by size:
3423 1304 710 69 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4129 4503 4649 387
Size: 1064 138 2540 374 146 1280
Fragments arranged by size:
2540 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2574 3605 4202 4989 5257 1239
Size: 393 542 400 1031 597 787 268 1524
Fragments arranged by size:
1524 1031 787 597 542 400 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2238 2238
Size: 5542
EcoRI G'AATT_C
Cuts at: 2084 2851 3229 2084
Size: 767 378 4397
Fragments arranged by size:
4397 767 378
FspI TGC'GCA
Cuts at: 4048 4048
Size: 5542
HaeII r_GCGC'y
Cuts at: 1678 4689 1678
Size: 3011 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 4183 4215 4257
Size: 32 135 32 437 32 2142 32 42
Cuts at: 4257 4289 1373
Size: 32 2626
Fragments arranged by size:
2626 2142 437 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2886 3132 669
Size: 988 281 948 246 3079
Fragments arranged by size:
3079 988 948 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5542
HpaI GTT'AAC
Cuts at: 3132 3132
Size: 5542
KpnI G_GTAC'C
Cuts at: 2867 2867
Size: 5542
MfeI C'AATT_G
Cuts at: 3141 3141
Size: 5542
MluI A'CGCG_T
Cuts at: 5418 5418
Size: 5542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2327 4656 4840 1305
Size: 1022 2329 184 2007
Fragments arranged by size:
2329 2007 1022 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3720
Fragments arranged by size:
3720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3500 3859 4018 518
Size: 648 2334 359 159 2042
Fragments arranged by size:
2334 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2958 3635 4783 5028 1606
Size: 1352 677 1148 245 2120
Fragments arranged by size:
2120 1352 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4990
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5542
NotI GC'GGCC_GC
Cuts at: 2989 2989
Size: 5542
NspI r_CATG'y
Cuts at: 1132 1229 2436 2466 2675 2900 4445 1132
Size: 97 1207 30 209 225 1545 2229
Fragments arranged by size:
2229 1545 1207 225 209 97 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5542
PciI A'CATG_T
Cuts at: 4441 4441
Size: 5542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 2109 2657 1108
Size: 933 68 548 3993
Fragments arranged by size:
3993 933 548 68
PmeI GTTT'AAAC
Cuts at: 2846 2846
Size: 5542
PmlI CAC'GTG
Cuts at: 2837 2837
Size: 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 5124 5156 3611
Size: 32 1481 32 3997
Fragments arranged by size:
3997 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5542
PsiI TTA'TAA
Cuts at: 3112 3112
Size: 5542
PstI C_TGCA'G
Cuts at: 830 2894 830
Size: 2064 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5542
SalI G'TCGA_C
Cuts at: 1936 2884 1936
Size: 948 4594
SapI GCTCTTCn'nnn_
Cuts at: 2063 2132 2063
Size: 69 5473
SbfI CC_TGCA'GG
Cuts at: 2894 2894
Size: 5542
ScaI AGT'ACT
Cuts at: 3790 5502 3790
Size: 1712 3830
SfcI C'TryA_G
Cuts at: 826 1045 2684 2890 4025 4706 4897 826
Size: 219 1639 206 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 681 219 206 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5542
SmaI CCC'GGG
Cuts at: 1872 2869 1872
Size: 997 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 2499 3646 4547 4809 820
Size: 197 926 390 166 1147 901 262 1553
Fragments arranged by size:
1553 1147 926 901 390 262 197 166
SnaBI TAC'GTA
Cuts at: 493 3333 493
Size: 2840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5542
SphI G_CATG'C
Cuts at: 1132 2466 2900 1132
Size: 1334 434 3774
Fragments arranged by size:
3774 1334 434
SspI AAT'ATT
Cuts at: 5 52 2645 3466 5
Size: 47 2593 821 2081
Fragments arranged by size:
2593 2081 821 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5542
StyI C'CwwG_G
Cuts at: 513 1065 2564 2981 513
Size: 552 1499 417 3074
Fragments arranged by size:
3074 1499 552 417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3569 3586 3739 3924 4369 325
Size: 1255 1989 17 153 185 445 1498
Fragments arranged by size:
1989 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3788 5500 96
Size: 274 80 33 51 1443 1811 1712 138
Fragments arranged by size:
1811 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2371 2403 2560 2592 5502 5534 2371
Size: 32 157 32 2910 32 2379
Fragments arranged by size:
2910 2379 157 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5542
XbaI T'CTAG_A
Cuts at: 2878 2878
Size: 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5542
XmaI C'CCGG_G
Cuts at: 1870 2867 1870
Size: 997 4545
XmnI GAAnn'nnTTC
Cuts at: 2057 3671 2057
Size: 1614 3928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 276
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI