Restriction map for
pFN21AE6281
(Circular) (Six-base) MAPSORT of: pFN21AE6281.seq Check: 2924 from: 1 to: 5461
pFN21AE6281 5461 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2658 2804 1937
Size: 721 146 4594
Fragments arranged by size:
4594 721 146
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2382 3524 4908 5176 1632
Size: 750 1142 1384 268 1917
Fragments arranged by size:
1917 1384 1142 750 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 2088
Size: 32 5429
AlwNI CAG_nnn'CTG
Cuts at: 2420 3204 4776 2420
Size: 784 1572 3105
Fragments arranged by size:
3105 1572 784
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2786 1870
Size: 73 843 4545
Fragments arranged by size:
4545 843 73
AvrII C'CTAG_G
Cuts at: 2159 2159
Size: 5461
BamHI G'GATC_C
Cuts at: 2186 2791 3158 2186
Size: 605 367 4489
Fragments arranged by size:
4489 605 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 4237 618
Size: 325 217 627 995 1455 1842
Fragments arranged by size:
1842 1455 995 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2717 2780 1391
Size: 1326 63 4072
Fragments arranged by size:
4072 1326 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 928
Size: 971 471 4019
Fragments arranged by size:
4019 971 471
BbvCI CC'TCA_GC
Cuts at: 2024 2024
Size: 5461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4072 136
Size: 107 122 71 631 605 2400 1525
Fragments arranged by size:
2400 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2698 3522 4678 1790
Size: 908 824 1156 2573
Fragments arranged by size:
2573 1156 908 824
BmgBI CAC'GTC
Cuts at: 1318 2461 1318
Size: 1143 4318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2430 3192 4150
Size: 416 53 83 399 24 1013 762 958
Cuts at: 4150 442
Size: 1753
Fragments arranged by size:
1753 1013 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 2731 4121 1239
Size: 1117 375 1390 2579
Fragments arranged by size:
2579 1390 1117 375
Bpu10I CC'TnA_GC
Cuts at: 2024 2024
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 3586 4451 4749 2243
Size: 1343 865 298 2955
Fragments arranged by size:
2955 1343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4124 882
Size: 617 2625 2219
Fragments arranged by size:
2625 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3650 4285 275
Size: 53 83 186 936 1735 382 635 1451
Fragments arranged by size:
1735 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2717 3894 4292 4566 4713 5124 2717
Size: 1177 398 274 147 411 3054
Fragments arranged by size:
3054 1177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5236
Fragments arranged by size:
5236 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2264 2546 1186
Size: 871 207 282 4101
Fragments arranged by size:
4101 871 282 207
BseYI C'CCAG_C
Cuts at: 2053 4664 5160 2053
Size: 2611 496 2354
Fragments arranged by size:
2611 2354 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2386 1163
Size: 573 650 4238
Fragments arranged by size:
4238 650 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2092 2911 3672 4700 1540
Size: 275 186 87 4 819 761 1028 2301
Fragments arranged by size:
2301 1028 819 761 275 186 87 4
BsiHKAI G_wGCw'C
Cuts at: 2344 2717 3522 3607 4678 2344
Size: 373 805 85 1071 3127
Fragments arranged by size:
3127 1071 805 373 85
BsmI GAATG_Cn'
Cuts at: 2290 2320 2972 3065 2290
Size: 30 652 93 4686
Fragments arranged by size:
4686 652 93 30
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2344 2698 2717 2780 3522 3607 4678
Size: 399 554 354 19 63 742 85 1071
Cuts at: 4678 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 554 399 354 85 63
19
BspEI T'CCGG_A
Cuts at: 2717 5124 2717
Size: 2407 3054
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 844
Size: 1972 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2526 3348 1394
Size: 429 703 822 3507
Fragments arranged by size:
3507 822 703 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4105 4292 1700
Size: 110 2295 187 2869
Fragments arranged by size:
2869 2295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5461
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 2294 1184
Size: 1110 4351
BstYI r'GATC_y
Cuts at: 1972 2186 2791 3158 3543 3560 5012 5098 5110
Size: 214 605 367 385 17 1452 86 12
Cuts at: 5110 5456 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 605 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2289 2652 513
Size: 552 939 285 363 3322
Fragments arranged by size:
3322 939 552 363 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2977 3771 3791 2977
Size: 794 20 4647
Fragments arranged by size:
4647 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2301 2908
Size: 54 1425 50 275 18 255 216 607
Cuts at: 2908 3797 5426 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 607 275 255 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2085 2908 1537
Size: 275 273 823 4090
Fragments arranged by size:
4090 823 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 2642 3391 1353
Size: 1289 749 3423
Fragments arranged by size:
3423 1289 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 2546 4048 4422 4568 387
Size: 1064 138 455 502 1502 374 146 1280
Fragments arranged by size:
1502 1280 1064 502 455 374 146 138
EcoICRI GAG'CTC
Cuts at: 2715 2715
Size: 5461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2382 2731 3524 4121 4908 5176
Size: 393 724 26 349 793 597 787 268
Cuts at: 5176 1239
Size: 1524
Fragments arranged by size:
1524 793 787 724 597 393 349 268
26
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 2243
Size: 5461
EcoO109I rG'GnC_Cy
Cuts at: 2077 2077
Size: 5461
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 2702 3967 2702
Size: 1265 4196
HaeII r_GCGC'y
Cuts at: 1678 4608 1678
Size: 2930 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 4102 4134 4176
Size: 32 135 32 477 32 2021 32 42
Cuts at: 4176 4208 1373
Size: 32 2626
Fragments arranged by size:
2626 2021 477 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4575 4759 1305
Size: 3270 184 2007
Fragments arranged by size:
3270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 10
Size: 54 1425 343 471 3168
Fragments arranged by size:
3168 1425 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2197 2321 2550 2737 2877 3554 4702
Size: 408 183 124 229 187 140 677 1148
Cuts at: 4702 4947 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 408 245 229 187 183
140 124
NcoI C'CATG_G
Cuts at: 513 1065 2004 2652 513
Size: 552 939 648 3322
Fragments arranged by size:
3322 939 648 552
NdeI CA'TA_TG
Cuts at: 387 2752 387
Size: 2365 3096
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2545 2819 4364 1132
Size: 97 1316 274 1545 2229
Fragments arranged by size:
2229 1545 1316 274 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2077
Size: 5461
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2813 830
Size: 1983 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
PvuII CAG'CTG
Cuts at: 2014 2197 2737 2014
Size: 183 540 4738
Fragments arranged by size:
4738 540 183
SacI G_AGCT'C
Cuts at: 2717 2717
Size: 5461
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2809 3944 4625 4816 826
Size: 219 1764 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2788 1872
Size: 916 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 3565 4466 4728 820
Size: 197 926 315 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 315 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2545 2819 1132
Size: 1413 274 3774
Fragments arranged by size:
3774 1413 274
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 2537 1517
Size: 1020 4441
StyI C'CwwG_G
Cuts at: 513 1065 2004 2159 2244 2584 2652 2900 513
Size: 552 939 155 85 340 68 248 3074
Fragments arranged by size:
3074 939 552 340 248 155 85 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 3488 3505 3658 3843 4288 325
Size: 1255 526 1382 17 153 185 445 1498
Fragments arranged by size:
1498 1382 1255 526 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2296 2296
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2786 1870
Size: 916 4545
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI