Restriction map for
pFN21AE6274
(Circular) (Six-base) MAPSORT of: pFN21AE6274.seq Check: 8082 from: 1 to: 5275
pFN21AE6274 5275 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3085 278
Size: 53 83 186 936 1549 2468
Fragments arranged by size:
2468 1549 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2618 1937
Size: 681 4594
Acc65I G'GTAC_C
Cuts at: 2596 2596
Size: 5275
AclI AA'CG_TT
Cuts at: 1563 3402 3775 1563
Size: 1839 373 3063
Fragments arranged by size:
3063 1839 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 3338 4722 4990 1632
Size: 535 1171 1384 268 1917
Fragments arranged by size:
1917 1384 1171 535 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5078
AflIII A'CryG_T
Cuts at: 1203 4174 5151 1203
Size: 2971 977 1327
Fragments arranged by size:
2971 1327 977
AgeI A'CCGG_T
Cuts at: 2365 4106 2365
Size: 1741 3534
AhdI GACnn_n'nnGTC
Cuts at: 2308 4004 2308
Size: 1696 3579
AleI CACnn'nnGTG
Cuts at: 1166 2279 1166
Size: 1113 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5275
AlwNI CAG_nnn'CTG
Cuts at: 3018 4590 3018
Size: 1572 3703
ApaI G_GGCC'C
Cuts at: 2101 2269 2101
Size: 168 5107
ApaLI G'TGCA_C
Cuts at: 3332 4488 3332
Size: 1156 4119
ApoI r'AATT_y
Cuts at: 1493 2417 2584 2810 2962 5071 1493
Size: 924 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 3829 160
Size: 872 2797 1606
Fragments arranged by size:
2797 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5275
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 1870
Size: 73 657 4545
Fragments arranged by size:
4545 657 73
BamHI G'GATC_C
Cuts at: 2206 2605 2972 2206
Size: 399 367 4509
Fragments arranged by size:
4509 399 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2596 4051 618
Size: 325 217 627 809 1455 1842
Fragments arranged by size:
1842 1455 809 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2101 2231 2269 2594 1391
Size: 710 130 38 325 4072
Fragments arranged by size:
4072 710 325 130 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2402 2436 3466 3500 1903
Size: 34 465 34 1030 34 3678
Fragments arranged by size:
3678 1030 465 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3167 4377 3167
Size: 1210 4065
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3886 136
Size: 107 122 71 631 605 2214 1525
Fragments arranged by size:
2214 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5270 5270
Size: 5275
BlpI GC'TnA_GC
Cuts at: 2692 2692
Size: 5275
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2269 3336 4492 1790
Size: 311 168 1067 1156 2573
Fragments arranged by size:
2573 1156 1067 311 168
BmgBI CAC'GTC
Cuts at: 1318 2125 1318
Size: 807 4468
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3006 3964 442
Size: 416 53 83 399 24 1589 958 1753
Fragments arranged by size:
1753 1589 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2420 3935 1239
Size: 995 186 1515 2579
Fragments arranged by size:
2579 1515 995 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3400 4265 4563 2333
Size: 1067 865 298 3045
Fragments arranged by size:
3045 1067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3938 882
Size: 617 2439 2219
Fragments arranged by size:
2439 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2488 3066 493
Size: 711 1284 578 2702
Fragments arranged by size:
2702 1284 711 578
BsaBI GATnn'nnATC
Cuts at: 2030 5269 2030
Size: 3239 2036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3082 3464 4099 275
Size: 53 83 186 936 1549 382 635 1451
Fragments arranged by size:
1549 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2365 3708 4106 4380 4527 4938 2365
Size: 1343 398 274 147 411 2702
Fragments arranged by size:
2702 1343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5050
Fragments arranged by size:
5050 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 4392
BseYI C'CCAG_C
Cuts at: 2232 2514 4478 4974 2232
Size: 282 1964 496 2533
Fragments arranged by size:
2533 1964 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 2540 1163
Size: 573 369 435 3898
Fragments arranged by size:
3898 573 435 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 3486 4514 1540
Size: 275 186 724 761 1028 2301
Fragments arranged by size:
2301 1028 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 3336 3421 4492 2322
Size: 1014 85 1071 3105
Fragments arranged by size:
3105 1071 1014 85
BsmI GAATG_Cn'
Cuts at: 2786 2879 2786
Size: 93 5182
BsmBI CGTCTCn'nnnn_
Cuts at: 2435 2435
Size: 5275
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2231 2269 2322 2594 3336 3421
Size: 399 311 130 38 53 272 742 85
Cuts at: 3421 4492 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 311 272 130 85
53 38
BspEI T'CCGG_A
Cuts at: 4938 4938
Size: 5275
BspHI T'CATG_A
Cuts at: 3164 4894 3164
Size: 1730 3545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2515 2630 844
Size: 1454 217 115 3489
Fragments arranged by size:
3489 1454 217 115
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 2224 3162 1394
Size: 429 224 177 938 3507
Fragments arranged by size:
3507 938 429 224 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 3770 3944 65
Size: 1183 2522 174 1396
Fragments arranged by size:
2522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2365 2548 2572 3919 4106 1700
Size: 110 555 183 24 1347 187 2869
Fragments arranged by size:
2869 1347 555 187 183 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5275
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5275
BssSI C'ACGA_G
Cuts at: 3335 4347 3335
Size: 1012 4263
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5275
BstBI TT'CG_AA
Cuts at: 4079 4079
Size: 5275
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5275
BstYI r'GATC_y
Cuts at: 1972 2206 2379 2605 2972 3357 3374 4826 4912
Size: 234 173 226 367 385 17 1452 86
Cuts at: 4912 4924 5270 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 234 226 173
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3784
Fragments arranged by size:
3784 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4536
Fragments arranged by size:
4536 683 56
BtsI GCAGTG_nn'
Cuts at: 2555 2791 3585 3605 2555
Size: 236 794 20 4225
Fragments arranged by size:
4225 794 236 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2449 2484 529
Size: 35 1885 35 3320
Fragments arranged by size:
3320 1885 35 35
DraI TTT'AAA
Cuts at: 2579 2579
Size: 5275
DraIII CAC_nnn'GTG
Cuts at: 2359 2359
Size: 5275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4282 809
Size: 507 2966 1802
Fragments arranged by size:
2966 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2169 2550
Size: 54 1425 50 275 18 303 36 381
Cuts at: 2550 2722 3611 5240 8
Size: 172 889 1629 43
Fragments arranged by size:
1629 1425 889 381 303 275 172 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 1537
Size: 275 910 4090
Fragments arranged by size:
4090 910 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2569 3205 1353
Size: 1216 636 3423
Fragments arranged by size:
3423 1216 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 3862 4236 4382 387
Size: 1064 138 495 1778 374 146 1280
Fragments arranged by size:
1778 1280 1064 495 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2234 2420 3338 3935 4722 4990
Size: 393 535 67 186 918 597 787 268
Cuts at: 4990 1239
Size: 1524
Fragments arranged by size:
1524 918 787 597 535 393 268 186
67
EcoO109I rG'GnC_Cy
Cuts at: 2265 2266 2527 2265
Size: 1 261 5013
Fragments arranged by size:
5013 261 1
EcoRI G'AATT_C
Cuts at: 2584 2962 2584
Size: 378 4897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 5275
FspI TGC'GCA
Cuts at: 3781 3781
Size: 5275
HaeII r_GCGC'y
Cuts at: 1678 4422 1678
Size: 2744 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2294 2326 3916
Size: 32 135 32 473 32 217 32 1590
Cuts at: 3916 3948 3990 4022 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1590 473 217 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2619 2865 669
Size: 988 281 681 246 3079
Fragments arranged by size:
3079 988 681 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5275
HpaI GTT'AAC
Cuts at: 2865 2865
Size: 5275
KpnI G_GTAC'C
Cuts at: 2600 2600
Size: 5275
MfeI C'AATT_G
Cuts at: 2874 2874
Size: 5275
MluI A'CGCG_T
Cuts at: 5151 5151
Size: 5275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4389 4573 1305
Size: 3084 184 2007
Fragments arranged by size:
3084 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 10
Size: 54 1425 343 303 3150
Fragments arranged by size:
3150 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2279 3233 3592 3751 518
Size: 648 975 138 954 359 159 2042
Fragments arranged by size:
2042 975 954 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2115 2236 2495 2691 3368 4516 4761 1606
Size: 509 121 259 196 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 509 259 245 196 121
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3784
Fragments arranged by size:
3784 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5275
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5275
NotI GC'GGCC_GC
Cuts at: 2722 2722
Size: 5275
NspI r_CATG'y
Cuts at: 1132 1229 2633 4178 1132
Size: 97 1404 1545 2229
Fragments arranged by size:
2229 1545 1404 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5275
PciI A'CATG_T
Cuts at: 4174 4174
Size: 5275
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5275
PmeI GTTT'AAAC
Cuts at: 2579 2579
Size: 5275
PmlI CAC'GTG
Cuts at: 2488 2488
Size: 5275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2150 2182 3344 3376 4857 4889 2150
Size: 32 1162 32 1481 32 2536
Fragments arranged by size:
2536 1481 1162 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2527
Size: 5275
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5275
PsiI TTA'TAA
Cuts at: 2845 2845
Size: 5275
PspOMI G'GGCC_C
Cuts at: 2097 2265 2097
Size: 168 5107
PstI C_TGCA'G
Cuts at: 830 2627 830
Size: 1797 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5275
PvuII CAG'CTG
Cuts at: 2115 2236 2115
Size: 121 5154
SalI G'TCGA_C
Cuts at: 1936 2617 1936
Size: 681 4594
SbfI CC_TGCA'GG
Cuts at: 2627 2627
Size: 5275
ScaI AGT'ACT
Cuts at: 2180 3523 5235 2180
Size: 1343 1712 2220
Fragments arranged by size:
2220 1712 1343
SfcI C'TryA_G
Cuts at: 826 1045 2479 2623 3758 4439 4630 826
Size: 219 1434 144 1135 681 191 1471
Fragments arranged by size:
1471 1434 1135 681 219 191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5275
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5275
SmaI CCC'GGG
Cuts at: 1872 2602 1872
Size: 730 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3379 4280 4542 820
Size: 197 926 405 1031 901 262 1553
Fragments arranged by size:
1553 1031 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 3066 493
Size: 2573 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5275
SphI G_CATG'C
Cuts at: 1132 2633 1132
Size: 1501 3774
SspI AAT'ATT
Cuts at: 5 52 3199 5
Size: 47 3147 2081
Fragments arranged by size:
3147 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5275
StyI C'CwwG_G
Cuts at: 513 1065 2004 2714 513
Size: 552 939 710 3074
Fragments arranged by size:
3074 939 710 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2506 3302 3319 3472 3657 4102 325
Size: 1255 926 796 17 153 185 445 1498
Fragments arranged by size:
1498 1255 926 796 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 2370 3521
Size: 274 80 33 51 1443 201 192 1151
Cuts at: 3521 5233 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1151 274 201 192 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5235 5267 5235
Size: 32 5243
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5275
XbaI T'CTAG_A
Cuts at: 2611 2611
Size: 5275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5275
XmaI C'CCGG_G
Cuts at: 1870 2600 1870
Size: 730 4545
XmnI GAAnn'nnTTC
Cuts at: 3404 3404
Size: 5275
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3083 276
Size: 53 83 186 936 1549 2468
Fragments arranged by size:
2468 1549 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI