(Circular) (Six-base) MAPSORT of: pFN21AE6270.seq Check: 5604 from: 1 to: 5281 pFN21AE6270 5281 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3091 278 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 1937 Size: 687 4594 Acc65I G'GTAC_C Cuts at: 2602 2602 Size: 5281 AclI AA'CG_TT Cuts at: 1563 3408 3781 1563 Size: 1845 373 3063 Fragments arranged by size: 3063 1845 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 3344 4728 4996 1632 Size: 458 1254 1384 268 1917 Fragments arranged by size: 1917 1384 1254 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5084 AflIII A'CryG_T Cuts at: 1203 4180 5157 1203 Size: 2977 977 1327 Fragments arranged by size: 2977 1327 977 AgeI A'CCGG_T Cuts at: 4112 4112 Size: 5281 AhdI GACnn_n'nnGTC Cuts at: 4010 4010 Size: 5281 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5281 AlwNI CAG_nnn'CTG Cuts at: 3024 4596 3024 Size: 1572 3709 ApaLI G'TGCA_C Cuts at: 3338 4494 3338 Size: 1156 4125 ApoI r'AATT_y Cuts at: 1493 2251 2369 2522 2590 2816 2968 5077 1493 Size: 758 118 153 68 226 152 2109 1697 Fragments arranged by size: 2109 1697 758 226 153 152 118 68 AseI AT'TA_AT Cuts at: 160 1032 3835 160 Size: 872 2803 1606 Fragments arranged by size: 2803 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 AvaI C'yCGr_G Cuts at: 1870 1943 2606 1870 Size: 73 663 4545 Fragments arranged by size: 4545 663 73 BamHI G'GATC_C Cuts at: 2611 2978 2611 Size: 367 4914 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2602 4057 618 Size: 325 217 627 231 584 1455 1842 Fragments arranged by size: 1842 1455 627 584 325 231 217 BanII G_rGCy'C Cuts at: 1391 2032 2183 2600 1391 Size: 641 151 417 4072 Fragments arranged by size: 4072 641 417 151 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 5281 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2268 928 Size: 971 222 147 3941 Fragments arranged by size: 3941 971 222 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3472 3506 1903 Size: 34 1535 34 3678 Fragments arranged by size: 3678 1535 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3173 4383 3173 Size: 1210 4071 BclI T'GATC_A Cuts at: 1548 1548 Size: 5281 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 3892 136 Size: 107 122 71 631 605 355 1865 1525 Fragments arranged by size: 1865 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 5276 5276 Size: 5281 BlpI GC'TnA_GC Cuts at: 2698 2698 Size: 5281 Bme1580I G_kGCm'C Cuts at: 1790 3342 4498 1790 Size: 1552 1156 2573 Fragments arranged by size: 2573 1552 1156 BmgBI CAC'GTC Cuts at: 1318 2452 1318 Size: 1134 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3012 3970 442 Size: 416 53 83 399 24 1595 958 1753 Fragments arranged by size: 1753 1595 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5281 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2252 2252 Size: 5281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3941 1239 Size: 2702 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3406 4271 4569 3406 Size: 865 298 4118 Fragments arranged by size: 4118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2226 3944 882 Size: 617 727 1718 2219 Fragments arranged by size: 2219 1718 727 617 BsaAI yAC'GTr Cuts at: 493 1204 3072 493 Size: 711 1868 2702 Fragments arranged by size: 2702 1868 711 BsaBI GATnn'nnATC Cuts at: 5275 5275 Size: 5281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 3088 3470 4105 Size: 53 83 186 936 486 1069 382 635 Cuts at: 4105 275 Size: 1451 Fragments arranged by size: 1451 1069 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3714 4112 4386 4533 4944 3714 Size: 398 274 147 411 4051 Fragments arranged by size: 4051 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5056 Fragments arranged by size: 5056 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2244 1186 Size: 1058 4223 BseYI C'CCAG_C Cuts at: 4484 4980 4484 Size: 496 4785 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2118 1163 Size: 573 382 4326 Fragments arranged by size: 4326 573 382 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 3492 4520 1540 Size: 275 186 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 3342 3427 4498 2166 Size: 1176 85 1071 2949 Fragments arranged by size: 2949 1176 1071 85 BsmI GAATG_Cn' Cuts at: 2792 2885 2792 Size: 93 5188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2166 2183 2600 3342 3427 4498 Size: 399 242 134 17 417 742 85 1071 Cuts at: 4498 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 417 399 242 134 85 17 BspEI T'CCGG_A Cuts at: 4944 4944 Size: 5281 BspHI T'CATG_A Cuts at: 2139 2344 3170 4900 2139 Size: 205 826 1730 2520 Fragments arranged by size: 2520 1730 826 205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2636 844 Size: 1792 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3168 1394 Size: 429 1345 3507 Fragments arranged by size: 3507 1345 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3776 3950 65 Size: 1183 2528 174 1396 Fragments arranged by size: 2528 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2215 3925 4112 1700 Size: 110 405 1710 187 2869 Fragments arranged by size: 2869 1710 405 187 110 BsrGI T'GTAC_A Cuts at: 96 2415 96 Size: 2319 2962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5281 BssSI C'ACGA_G Cuts at: 3341 4353 3341 Size: 1012 4269 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5281 BstBI TT'CG_AA Cuts at: 4085 4085 Size: 5281 BstEII G'GTnAC_C Cuts at: 1184 2494 1184 Size: 1310 3971 BstXI CCAn_nnnn'nTGG Cuts at: 2191 2191 Size: 5281 BstYI r'GATC_y Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276 Size: 639 367 385 17 1452 86 12 346 Cuts at: 5276 1972 Size: 1977 Fragments arranged by size: 1977 1452 639 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2022 513 Size: 552 939 18 3772 Fragments arranged by size: 3772 939 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4542 Fragments arranged by size: 4542 683 56 BtsI GCAGTG_nn' Cuts at: 2179 2797 3591 3611 2179 Size: 618 794 20 3849 Fragments arranged by size: 3849 794 618 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5281 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5246 DraI TTT'AAA Cuts at: 2109 2296 2585 2109 Size: 187 289 4805 Fragments arranged by size: 4805 289 187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4288 809 Size: 507 2972 1802 Fragments arranged by size: 2972 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 3617 5246 Size: 54 1425 50 275 18 898 889 1629 Cuts at: 5246 8 Size: 43 Fragments arranged by size: 1629 1425 898 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2728 1537 Size: 275 916 4090 Fragments arranged by size: 4090 916 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2455 3211 1353 Size: 1102 756 3423 Fragments arranged by size: 3423 1102 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3868 4242 4388 387 Size: 1064 138 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 3344 3941 4728 4996 1239 Size: 393 458 1254 597 787 268 1524 Fragments arranged by size: 1524 1254 787 597 458 393 268 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 5281 EcoRI G'AATT_C Cuts at: 2590 2968 2590 Size: 378 4903 EcoRV GAT'ATC Cuts at: 2056 2056 Size: 5281 FspI TGC'GCA Cuts at: 3787 3787 Size: 5281 HaeII r_GCGC'y Cuts at: 1678 2022 2089 4428 1678 Size: 344 67 2339 2531 Fragments arranged by size: 2531 2339 344 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3922 3954 3996 4028 1373 Size: 32 135 32 2350 32 42 32 2626 Fragments arranged by size: 2626 2350 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2625 2871 669 Size: 988 281 687 246 3079 Fragments arranged by size: 3079 988 687 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5281 HpaI GTT'AAC Cuts at: 2871 2871 Size: 5281 KasI G'GCGC_C Cuts at: 2018 2018 Size: 5281 KpnI G_GTAC'C Cuts at: 2606 2606 Size: 5281 MfeI C'AATT_G Cuts at: 2880 2880 Size: 5281 MluI A'CGCG_T Cuts at: 5157 5157 Size: 5281 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4395 4579 1305 Size: 3090 184 2007 Fragments arranged by size: 3090 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3459 Fragments arranged by size: 3459 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2189 2504 3239 3598 3757 518 Size: 648 957 66 315 735 359 159 2042 Fragments arranged by size: 2042 957 735 648 359 315 159 66 MspA1I CmG'CkG Cuts at: 1606 2697 3374 4522 4767 1606 Size: 1091 677 1148 245 2120 Fragments arranged by size: 2120 1148 1091 677 245 NaeI GCC'GGC Cuts at: 2217 2217 Size: 5281 NarI GG'CG_CC Cuts at: 2019 2019 Size: 5281 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3790 Fragments arranged by size: 3790 939 552 NdeI CA'TA_TG Cuts at: 387 2482 2557 387 Size: 2095 75 3111 Fragments arranged by size: 3111 2095 75 NgoMIV G'CCGG_C Cuts at: 2215 2215 Size: 5281 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5281 NotI GC'GGCC_GC Cuts at: 2728 2728 Size: 5281 NspI r_CATG'y Cuts at: 1132 1229 2639 4184 1132 Size: 97 1410 1545 2229 Fragments arranged by size: 2229 1545 1410 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5281 PciI A'CATG_T Cuts at: 4180 4180 Size: 5281 PflMI CCAn_nnn'nTGG Cuts at: 1108 2486 1108 Size: 1378 3903 PfoI T'CCnGG_A Cuts at: 2256 2256 Size: 5281 PmeI GTTT'AAAC Cuts at: 2585 2585 Size: 5281 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 4863 4895 3350 Size: 32 1481 32 3736 Fragments arranged by size: 3736 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 5281 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5281 PsiI TTA'TAA Cuts at: 2851 2851 Size: 5281 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2325 2357 2325 Size: 32 5249 PstI C_TGCA'G Cuts at: 830 2633 830 Size: 1803 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5281 SalI G'TCGA_C Cuts at: 1936 2623 1936 Size: 687 4594 SapI GCTCTTCn'nnn_ Cuts at: 2455 2455 Size: 5281 SbfI CC_TGCA'GG Cuts at: 2633 2633 Size: 5281 ScaI AGT'ACT Cuts at: 3529 5241 3529 Size: 1712 3569 SexAI A'CCwGG_T Cuts at: 2100 2100 Size: 5281 SfcI C'TryA_G Cuts at: 826 1045 2629 3764 4445 4636 826 Size: 219 1584 1135 681 191 1471 Fragments arranged by size: 1584 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2020 2020 Size: 5281 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5281 SmaI CCC'GGG Cuts at: 1872 2608 1872 Size: 736 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3385 4286 4548 820 Size: 197 926 1442 901 262 1553 Fragments arranged by size: 1553 1442 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3072 493 Size: 2579 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5281 SphI G_CATG'C Cuts at: 1132 2639 1132 Size: 1507 3774 SspI AAT'ATT Cuts at: 5 52 3205 5 Size: 47 3153 2081 Fragments arranged by size: 3153 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5281 StyI C'CwwG_G Cuts at: 513 1065 2004 2720 513 Size: 552 939 716 3074 Fragments arranged by size: 3074 939 716 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 3308 3325 3478 3663 4108 325 Size: 1255 960 768 17 153 185 445 1498 Fragments arranged by size: 1498 1255 960 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 3527 5239 Size: 274 80 33 51 1443 438 1112 1712 Cuts at: 5239 96 Size: 138 Fragments arranged by size: 1712 1443 1112 438 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5241 5273 5241 Size: 32 5249 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5281 XbaI T'CTAG_A Cuts at: 2617 2617 Size: 5281 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5281 XmaI C'CCGG_G Cuts at: 1870 2606 1870 Size: 736 4545 XmnI GAAnn'nnTTC Cuts at: 3410 3410 Size: 5281 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3089 276 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI