Restriction map for
pFN21AE6269
(Circular) (Six-base) MAPSORT of: pFN21AE6269.seq Check: 9764 from: 1 to: 5470
pFN21AE6269 5470 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2049 2434 2049
Size: 385 5085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 1937
Size: 876 4594
Acc65I G'GTAC_C
Cuts at: 2791 2791
Size: 5470
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2780 3533 4917 5185 1632
Size: 1148 753 1384 268 1917
Fragments arranged by size:
1917 1384 1148 753 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 4301 4301
Size: 5470
AhdI GACnn_n'nnGTC
Cuts at: 4199 4199
Size: 5470
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5470
AlwNI CAG_nnn'CTG
Cuts at: 3213 4785 3213
Size: 1572 3898
ApaI G_GGCC'C
Cuts at: 2537 2594 2687 2537
Size: 57 93 5320
Fragments arranged by size:
5320 93 57
ApaLI G'TGCA_C
Cuts at: 3527 4683 3527
Size: 1156 4314
ApoI r'AATT_y
Cuts at: 1493 2018 2779 3005 3157 5266 1493
Size: 525 761 226 152 2109 1697
Fragments arranged by size:
2109 1697 761 525 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2342 2354 2766 2795 1870
Size: 73 399 12 412 29 4545
Fragments arranged by size:
4545 412 399 73 29 12
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2510 2791 4246 618
Size: 325 217 627 355 368 281 1455 1842
Fragments arranged by size:
1842 1455 627 368 355 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2120 2220 2537 2594 2687 2789 1391
Size: 729 100 317 57 93 102 4072
Fragments arranged by size:
4072 729 317 102 100 93 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2531 2750 928
Size: 971 632 219 3648
Fragments arranged by size:
3648 971 632 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3661 3695 1903
Size: 34 1724 34 3678
Fragments arranged by size:
3678 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5470
BfrBI ATG'CAT
Cuts at: 2405 2505 2405
Size: 100 5370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4081 136
Size: 107 122 71 631 605 2409 1525
Fragments arranged by size:
2409 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5465 5465
Size: 5470
BlpI GC'TnA_GC
Cuts at: 2887 2887
Size: 5470
Bme1580I G_kGCm'C
Cuts at: 1790 2537 2594 2687 3531 4687 1790
Size: 747 57 93 844 1156 2573
Fragments arranged by size:
2573 1156 844 747 93 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 2335 3201
Size: 416 53 83 399 24 767 151 866
Cuts at: 3201 4159 442
Size: 958 1753
Fragments arranged by size:
1753 958 866 767 416 399 151 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2622 4130 1239
Size: 1383 1508 2579
Fragments arranged by size:
2579 1508 1383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3595 4460 4758 3595
Size: 865 298 4307
Fragments arranged by size:
4307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2335 4133 882
Size: 617 836 1798 2219
Fragments arranged by size:
2219 1798 836 617
BsaAI yAC'GTr
Cuts at: 493 1204 3261 493
Size: 711 2057 2702
Fragments arranged by size:
2702 2057 711
BsaBI GATnn'nnATC
Cuts at: 5464 5464
Size: 5470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3277 3659 4294 275
Size: 53 83 186 936 1744 382 635 1451
Fragments arranged by size:
1744 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3903 4301 4575 4722 5133 3903
Size: 398 274 147 411 4240
Fragments arranged by size:
4240 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5245
Fragments arranged by size:
5245 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2402 1186
Size: 1120 96 4254
Fragments arranged by size:
4254 1120 96
BseYI C'CCAG_C
Cuts at: 2113 2233 2585 2680 4673 5169 2113
Size: 120 352 95 1993 496 2414
Fragments arranged by size:
2414 1993 496 352 120 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 1163
Size: 573 375 4522
Fragments arranged by size:
4522 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2920 3681 4709 1540
Size: 275 186 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3531 3616 4687 3531
Size: 85 1071 4314
Fragments arranged by size:
4314 1071 85
BsmI GAATG_Cn'
Cuts at: 2407 2981 3074 2407
Size: 574 93 4803
Fragments arranged by size:
4803 574 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2220 2537 2594 2687 2789 3531
Size: 399 330 100 317 57 93 102 742
Cuts at: 3531 3616 4687 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 317 102 100
93 85 57
BspEI T'CCGG_A
Cuts at: 5133 5133
Size: 5470
BspHI T'CATG_A
Cuts at: 3359 5089 3359
Size: 1730 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2434 2644 2825 844
Size: 1205 385 210 181 3489
Fragments arranged by size:
3489 1205 385 210 181
BsrBI CCG'CTC
Cuts at: 1394 1823 3357 1394
Size: 429 1534 3507
Fragments arranged by size:
3507 1534 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2032 3965 4139 65
Size: 1183 784 1933 174 1396
Fragments arranged by size:
1933 1396 1183 784 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2188 4114 4301 1700
Size: 110 378 1926 187 2869
Fragments arranged by size:
2869 1926 378 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5470
BssSI C'ACGA_G
Cuts at: 2219 3530 4542 2219
Size: 1311 1012 3147
Fragments arranged by size:
3147 1311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5470
BstBI TT'CG_AA
Cuts at: 4274 4274
Size: 5470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstYI r'GATC_y
Cuts at: 1972 2670 2800 3167 3552 3569 5021 5107 5119
Size: 698 130 367 385 17 1452 86 12
Cuts at: 5119 5465 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 698 385 367 346 130 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2385
Size: 5470
BtgI C'CryG_G
Cuts at: 513 1065 2200 2571 513
Size: 552 1135 371 3412
Fragments arranged by size:
3412 1135 552 371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3780 3800 2986
Size: 794 20 4656
Fragments arranged by size:
4656 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2774 2774
Size: 5470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 2646 2917
Size: 54 1425 50 275 18 537 279 271
Cuts at: 2917 3806 5435 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 537 279 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 1537
Size: 275 1105 4090
Fragments arranged by size:
4090 1105 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 3400 1353
Size: 779 1268 3423
Fragments arranged by size:
3423 1268 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4057 4431 4577 387
Size: 1064 138 2468 374 146 1280
Fragments arranged by size:
2468 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 2780 3533 4130 4917 5185 1239
Size: 393 990 158 753 597 787 268 1524
Fragments arranged by size:
1524 990 787 753 597 393 268 158
EcoO109I rG'GnC_Cy
Cuts at: 2533 2534 2590 2591 2533
Size: 1 56 1 5412
Fragments arranged by size:
5412 56 1 1
EcoRI G'AATT_C
Cuts at: 2779 3157 2779
Size: 378 5092
EcoRV GAT'ATC
Cuts at: 2578 2578
Size: 5470
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 4617 1678
Size: 2939 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4111 4143 4185 4217 1373
Size: 32 135 32 2539 32 42 32 2626
Fragments arranged by size:
2626 2539 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2814 3060 669
Size: 988 281 876 246 3079
Fragments arranged by size:
3079 988 876 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5470
HpaI GTT'AAC
Cuts at: 3060 3060
Size: 5470
KpnI G_GTAC'C
Cuts at: 2795 2795
Size: 5470
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2689 4584 4768 1305
Size: 1384 1895 184 2007
Fragments arranged by size:
2007 1895 1384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 2648 10
Size: 54 1425 343 537 279 2832
Fragments arranged by size:
2832 1425 537 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3428 3787 3946 518
Size: 648 2262 359 159 2042
Fragments arranged by size:
2262 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2338 2886 3563 4711 4956 1606
Size: 732 548 677 1148 245 2120
Fragments arranged by size:
2120 1148 732 677 548 245
NaeI GCC'GGC
Cuts at: 2190 2190
Size: 5470
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4918
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NgoMIV G'CCGG_C
Cuts at: 2188 2188
Size: 5470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NsiI A_TGCA'T
Cuts at: 2407 2507 2407
Size: 100 5370
NspI r_CATG'y
Cuts at: 1132 1229 2828 4373 1132
Size: 97 1599 1545 2229
Fragments arranged by size:
2229 1599 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5470
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 2655 1108
Size: 1098 449 3923
Fragments arranged by size:
3923 1098 449
PfoI T'CCnGG_A
Cuts at: 2430 2430
Size: 5470
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2378 2410 3539 3571 5052 5084 2378
Size: 32 1129 32 1481 32 2764
Fragments arranged by size:
2764 1481 1129 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5470
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PspOMI G'GGCC_C
Cuts at: 2533 2590 2683 2533
Size: 57 93 5320
Fragments arranged by size:
5320 93 57
PstI C_TGCA'G
Cuts at: 830 2187 2822 830
Size: 1357 635 3478
Fragments arranged by size:
3478 1357 635
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
PvuII CAG'CTG
Cuts at: 2338 2338
Size: 5470
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SbfI CC_TGCA'GG
Cuts at: 2822 2822
Size: 5470
ScaI AGT'ACT
Cuts at: 3718 5430 3718
Size: 1712 3758
SexAI A'CCwGG_T
Cuts at: 2304 2304
Size: 5470
SfcI C'TryA_G
Cuts at: 826 1045 2183 2818 3953 4634 4825 826
Size: 219 1138 635 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 635 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5470
SmaI CCC'GGG
Cuts at: 1872 2797 1872
Size: 925 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3574 4475 4737 820
Size: 197 926 1631 901 262 1553
Fragments arranged by size:
1631 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2828 1132
Size: 1696 3774
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 2298 1517
Size: 781 4689
StyI C'CwwG_G
Cuts at: 513 1065 2364 2370 2909 513
Size: 552 1299 6 539 3074
Fragments arranged by size:
3074 1299 552 539 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2140 2498 3497 3514 3667 3852
Size: 1255 462 98 358 999 17 153 185
Cuts at: 3852 4297 325
Size: 445 1498
Fragments arranged by size:
1498 1255 999 462 445 358 185 153
98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3716 5428 96
Size: 274 80 33 51 1443 1739 1712 138
Fragments arranged by size:
1739 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2230 2262 2625 2657 5430 5462 2230
Size: 32 363 32 2773 32 2238
Fragments arranged by size:
2773 2238 363 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2795 1870
Size: 925 4545
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI