Restriction map for
pFN21AE6268
(Circular) (Six-base) MAPSORT of: pFN21AE6268.seq Check: 5225 from: 1 to: 5512
pFN21AE6268 5512 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2183 2567 2683 2183
Size: 384 116 5012
Fragments arranged by size:
5012 384 116
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2166 2833 2166
Size: 667 4845
AclI AA'CG_TT
Cuts at: 1563 2811 3639 4012 1563
Size: 1248 828 373 3063
Fragments arranged by size:
3063 1248 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3575 4959 5227 1632
Size: 1943 1384 268 1917
Fragments arranged by size:
1943 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 2033 1166
Size: 867 4645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 3255 4827 3255
Size: 1572 3940
ApaLI G'TGCA_C
Cuts at: 2324 2696 3569 4725 2324
Size: 372 873 1156 3111
Fragments arranged by size:
3111 1156 873 372
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2441 2833 4288 618
Size: 325 217 627 379 275 392 1455 1842
Fragments arranged by size:
1842 1455 627 392 379 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2047 2831 1391
Size: 656 784 4072
Fragments arranged by size:
4072 784 656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2749 928
Size: 971 850 3691
Fragments arranged by size:
3691 971 850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3703 3737 1903
Size: 34 1766 34 3678
Fragments arranged by size:
3678 1766 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4123 136
Size: 107 122 71 631 605 2451 1525
Fragments arranged by size:
2451 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2700 3573 4729 1790
Size: 538 372 873 1156 2573
Fragments arranged by size:
2573 1156 873 538 372
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3243 4201 442
Size: 416 53 83 399 24 1826 958 1753
Fragments arranged by size:
1826 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2221 2548 2617 4172 1239
Size: 822 160 327 69 1555 2579
Fragments arranged by size:
2579 1555 822 327 160 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 2724 2773 4175 882
Size: 617 641 584 49 1402 2219
Fragments arranged by size:
2219 1402 641 617 584 49
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3701 4336 275
Size: 53 83 186 936 1786 382 635 1451
Fragments arranged by size:
1786 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2336 2354 2366 2381
Size: 30 165 30 921 12 18 12 15
Cuts at: 2381 2411 2735 2765 1178
Size: 30 324 30 3925
Fragments arranged by size:
3925 921 324 165 30 30 30 30
18 15 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 1186
Size: 1038 4474
BseYI C'CCAG_C
Cuts at: 2202 2257 2481 2612 4715 5211 2202
Size: 55 224 131 2103 496 2503
Fragments arranged by size:
2503 2103 496 224 131 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4939
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2686 2962 3723 4751 1540
Size: 275 186 685 276 761 1028 2301
Fragments arranged by size:
2301 1028 761 685 276 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2700 3573 3658 4729 2328
Size: 372 873 85 1071 3111
Fragments arranged by size:
3111 1071 873 372 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
BsmBI CGTCTCn'nnnn_
Cuts at: 2401 2401
Size: 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2328 2700 2831 3573 3658 4729
Size: 399 257 281 372 131 742 85 1071
Cuts at: 4729 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 372 281 257 131
85
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2183 2567 2683 2867 844
Size: 1278 61 384 116 184 3489
Fragments arranged by size:
3489 1278 384 184 116 61
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 4007 4181 65
Size: 1183 1119 1640 174 1396
Fragments arranged by size:
1640 1396 1183 1119 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2262 2438 2532 2685 4156 4343 1700
Size: 110 452 176 94 153 1471 187 2869
Fragments arranged by size:
2869 1471 452 187 176 153 110 94
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2412 2842 3209 3594 3611 5063 5149 5161
Size: 440 430 367 385 17 1452 86 12
Cuts at: 5161 5507 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 440 430 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4960
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3028 3822 3842 2221
Size: 807 794 20 3891
Fragments arranged by size:
3891 807 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2683 2959 3848
Size: 54 1425 50 275 18 853 276 889
Cuts at: 3848 5477 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 853 276 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2683 2959 1537
Size: 275 871 276 4090
Fragments arranged by size:
4090 871 276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3442 1353
Size: 2089 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2221 2548 2617 3575 4172 4959
Size: 393 429 160 327 69 958 597 787
Cuts at: 4959 5227 1239
Size: 268 1524
Fragments arranged by size:
1524 958 787 597 429 393 327 268
160 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2525 2525
Size: 5512
EcoO109I rG'GnC_Cy
Cuts at: 2059 2184 2206 2241 2059
Size: 125 22 35 5330
Fragments arranged by size:
5330 125 35 22
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 4659 1678
Size: 2981 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 4153 4185 4227
Size: 32 135 32 752 32 1797 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 752 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KpnI G_GTAC'C
Cuts at: 2170 2837 2170
Size: 667 4845
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 4626 4810 1305
Size: 1062 2259 184 2007
Fragments arranged by size:
2259 2007 1062 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 3470 3829 3988 518
Size: 648 867 1437 359 159 2042
Fragments arranged by size:
2042 1437 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2121 2202 2419 2637 2928 3605 4753
Size: 417 98 81 217 218 291 677 1148
Cuts at: 4753 4998 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 417 291 245 218 217
98 81
NaeI GCC'GGC
Cuts at: 2264 2440 2264
Size: 176 5336
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4960
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NgoMIV G'CCGG_C
Cuts at: 2262 2438 2262
Size: 176 5336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2121 2202 1108
Size: 1013 81 4418
Fragments arranged by size:
4418 1013 81
PfoI T'CCnGG_A
Cuts at: 2237 2237
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2184 2206 2241 2059
Size: 125 22 35 5330
Fragments arranged by size:
5330 125 35 22
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2145 2496 2526 2864 830
Size: 1315 351 30 338 3478
Fragments arranged by size:
3478 1315 351 338 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
PvuII CAG'CTG
Cuts at: 2121 2202 2419 2637 2121
Size: 81 217 218 4996
Fragments arranged by size:
4996 218 217 81
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SanDI GG'GwC_CC
Cuts at: 2059 2184 2059
Size: 125 5387
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2141 2492 2522 2860 3995 4676 4867
Size: 219 1096 351 30 338 1135 681 191
Cuts at: 4867 826
Size: 1471
Fragments arranged by size:
1471 1135 1096 681 351 338 219 191
30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2951 513
Size: 552 1886 3074
Fragments arranged by size:
3074 1886 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3758 5470
Size: 274 80 33 51 1443 168 1613 1712
Cuts at: 5470 96
Size: 138
Fragments arranged by size:
1712 1613 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2735 2767 5472 5504 2735
Size: 32 2705 32 2743
Fragments arranged by size:
2743 2705 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI