Restriction map for
pFN21AE6266
(Circular) (Six-base) MAPSORT of: pFN21AE6266.seq Check: 9145 from: 1 to: 5467
pFN21AE6266 5467 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3277 278
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2712 2810 1937
Size: 775 98 4594
Fragments arranged by size:
4594 775 98
Acc65I G'GTAC_C
Cuts at: 2043 2788 2043
Size: 745 4722
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3530 4914 5182 1632
Size: 1898 1384 268 1917
Fragments arranged by size:
1917 1898 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 2511 4366 5343 1203
Size: 1308 1855 977 1327
Fragments arranged by size:
1855 1327 1308 977
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 4196 4196
Size: 5467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5467
AlwNI CAG_nnn'CTG
Cuts at: 3210 4782 3210
Size: 1572 3895
ApaI G_GGCC'C
Cuts at: 2634 2634
Size: 5467
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2729 2776 3002 3154 5263 1493
Size: 1236 47 226 152 2109 1697
Fragments arranged by size:
2109 1697 1236 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2173 2792 1870
Size: 73 230 619 4545
Fragments arranged by size:
4545 619 230 73
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2316 2788 4243 618
Size: 325 217 627 256 273 472 1455 1842
Fragments arranged by size:
1842 1455 627 472 325 273 256 217
BanII G_rGCy'C
Cuts at: 1391 2634 2786 1391
Size: 1243 152 4072
Fragments arranged by size:
4072 1243 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3658 3692 1903
Size: 34 1721 34 3678
Fragments arranged by size:
3678 1721 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3359 4569 3359
Size: 1210 4257
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2697 4078 136
Size: 107 122 71 631 605 1025 1381 1525
Fragments arranged by size:
1525 1381 1025 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5462 5462
Size: 5467
BlpI GC'TnA_GC
Cuts at: 2884 2884
Size: 5467
Bme1580I G_kGCm'C
Cuts at: 1790 2429 2634 3528 4684 1790
Size: 639 205 894 1156 2573
Fragments arranged by size:
2573 1156 894 639 205
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3198 4156 442
Size: 416 53 83 399 24 1781 958 1753
Fragments arranged by size:
1781 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4127 1239
Size: 2888 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 3592 4457 4755 2282
Size: 1310 865 298 2994
Fragments arranged by size:
2994 1310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4130 882
Size: 617 2631 2219
Fragments arranged by size:
2631 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3258 493
Size: 711 2054 2702
Fragments arranged by size:
2702 2054 711
BsaBI GATnn'nnATC
Cuts at: 5461 5461
Size: 5467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2178 3274 3656 4291
Size: 53 83 186 936 645 1096 382 635
Cuts at: 4291 275
Size: 1451
Fragments arranged by size:
1451 1096 936 645 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2400 3900 4298 4572 4719 5130 2400
Size: 1500 398 274 147 411 2737
Fragments arranged by size:
2737 1500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5242
Fragments arranged by size:
5242 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 4110
BseYI C'CCAG_C
Cuts at: 2245 2460 2567 4670 5166 2245
Size: 215 107 2103 496 2546
Fragments arranged by size:
2546 2103 496 215 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4894
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 3678 4706 1540
Size: 275 186 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3528 3613 4684 3528
Size: 85 1071 4311
Fragments arranged by size:
4311 1071 85
BsiWI C'GTAC_G
Cuts at: 2311 2311
Size: 5467
BsmI GAATG_Cn'
Cuts at: 2490 2978 3071 2490
Size: 488 93 4886
Fragments arranged by size:
4886 488 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2080 2080
Size: 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2429 2634 2786 3528 3613 4684 1391
Size: 399 639 205 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 399 205 152 85
BspEI T'CCGG_A
Cuts at: 2400 5130 2400
Size: 2730 2737
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 844
Size: 1978 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3354 1394
Size: 429 1531 3507
Fragments arranged by size:
3507 1531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 3962 4136 65
Size: 1183 1164 1550 174 1396
Fragments arranged by size:
1550 1396 1183 1164 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2582 4111 4298 1700
Size: 110 772 1529 187 2869
Fragments arranged by size:
2869 1529 772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 2577 794
Size: 1406 377 3684
Fragments arranged by size:
3684 1406 377
BstBI TT'CG_AA
Cuts at: 4271 4271
Size: 5467
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5467
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2245
Size: 5467
BstYI r'GATC_y
Cuts at: 1972 2066 2797 3164 3549 3566 5018 5104 5116
Size: 94 731 367 385 17 1452 86 12
Cuts at: 5116 5462 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 731 385 367 346 94 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2433 513
Size: 552 939 429 3547
Fragments arranged by size:
3547 939 552 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2201 2429 2507 2983 3777 3797 2201
Size: 228 78 476 794 20 3871
Fragments arranged by size:
3871 794 476 228 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5432
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DraIII CAC_nnn'GTG
Cuts at: 2739 2739
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2689 2914
Size: 54 1425 50 275 18 177 682 225
Cuts at: 2914 3803 5432 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 682 275 225 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2914 1537
Size: 275 1102 4090
Fragments arranged by size:
4090 1102 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3397 1353
Size: 2044 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2601 4054 4428 4574 387
Size: 1064 138 1012 1453 374 146 1280
Fragments arranged by size:
1453 1280 1064 1012 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3530 4127 4914 5182 1239
Size: 393 1898 597 787 268 1524
Fragments arranged by size:
1898 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2630 2631 2659 2630
Size: 1 28 5438
Fragments arranged by size:
5438 28 1
EcoRI G'AATT_C
Cuts at: 2776 3154 2776
Size: 378 5089
FspI TGC'GCA
Cuts at: 3973 3973
Size: 5467
HaeII r_GCGC'y
Cuts at: 1678 4614 1678
Size: 2936 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2325 2357 4108
Size: 32 135 32 486 32 235 32 1751
Cuts at: 4108 4140 4182 4214 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1751 486 235 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KpnI G_GTAC'C
Cuts at: 2047 2792 2047
Size: 745 4722
MfeI C'AATT_G
Cuts at: 3066 3066
Size: 5467
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 4581 4765 1305
Size: 1044 2232 184 2007
Fragments arranged by size:
2232 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2691 10
Size: 54 1425 343 177 682 2786
Fragments arranged by size:
2786 1425 682 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3425 3784 3943 518
Size: 648 2259 359 159 2042
Fragments arranged by size:
2259 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2435 2557 2883 3560 4708 4953 1606
Size: 829 122 326 677 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 326 245 122
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 5467
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3976
Fragments arranged by size:
3976 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5467
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 5467
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2575 2825 4370 1132
Size: 97 1346 250 1545 2229
Fragments arranged by size:
2229 1545 1346 250 97
PasI CC'CwG_GG
Cuts at: 1348 2025 1348
Size: 677 4790
PciI A'CATG_T
Cuts at: 4366 4366
Size: 5467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2293 1108
Size: 1185 4282
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 5049 5081 3536
Size: 32 1481 32 3922
Fragments arranged by size:
3922 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PspOMI G'GGCC_C
Cuts at: 2630 2630
Size: 5467
PstI C_TGCA'G
Cuts at: 830 2090 2117 2502 2540 2819 830
Size: 1260 27 385 38 279 3478
Fragments arranged by size:
3478 1260 385 279 38 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
SacII CC_GC'GG
Cuts at: 2436 2436
Size: 5467
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SfcI C'TryA_G
Cuts at: 826 1045 2086 2113 2498 2536 2815 3950 4631
Size: 219 1041 27 385 38 279 1135 681
Cuts at: 4631 4822 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1041 681 385 279 219 191
38 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 2582 1700
Size: 882 4585
SmaI CCC'GGG
Cuts at: 1872 2794 1872
Size: 922 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3571 4472 4734 820
Size: 197 926 318 1310 901 262 1553
Fragments arranged by size:
1553 1310 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5467
StyI C'CwwG_G
Cuts at: 513 1065 2004 2053 2906 513
Size: 552 939 49 853 3074
Fragments arranged by size:
3074 939 853 552 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 2334 3494 3511 3664 3849 4294
Size: 1255 608 146 1160 17 153 185 445
Cuts at: 4294 325
Size: 1498
Fragments arranged by size:
1498 1255 1160 608 445 185 153 146
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 3713 5425
Size: 274 80 33 51 1443 348 1388 1712
Cuts at: 5425 96
Size: 138
Fragments arranged by size:
1712 1443 1388 348 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 5427 5459 2205
Size: 32 3190 32 2213
Fragments arranged by size:
3190 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5467
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5467
XmaI C'CCGG_G
Cuts at: 1870 2792 1870
Size: 922 4545
XmnI GAAnn'nnTTC
Cuts at: 3596 3596
Size: 5467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3275 276
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI