Restriction map for
pFN21AE6265
(Circular) (Six-base) MAPSORT of: pFN21AE6265.seq Check: 8209 from: 1 to: 5497
pFN21AE6265 5497 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2137 2521 2600 2609 2743 2137
Size: 384 79 9 134 4891
Fragments arranged by size:
4891 384 134 79 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 2840 1937
Size: 807 96 4594
Fragments arranged by size:
4594 807 96
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 2796 3624 3997 1563
Size: 1233 828 373 3063
Fragments arranged by size:
3063 1233 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2077 2399 2475 3560 4944 5212 1632
Size: 445 322 76 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 445 322 268 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 2465 4396 5373 1203
Size: 1262 1931 977 1327
Fragments arranged by size:
1931 1327 1262 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 2681 1166
Size: 1515 3982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2308 2769 3240 4812 2308
Size: 461 471 1572 2993
Fragments arranged by size:
2993 1572 471 461
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 2748 2822 1870
Size: 73 376 429 74 4545
Fragments arranged by size:
4545 429 376 74 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2621 2708 2818 4273 618
Size: 325 217 627 834 87 110 1455 1842
Fragments arranged by size:
1842 1455 834 627 325 217 110 87
BanII G_rGCy'C
Cuts at: 1391 2135 2298 2582 2816 1391
Size: 744 163 284 234 4072
Fragments arranged by size:
4072 744 284 234 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2496 4108 136
Size: 107 122 71 631 605 824 1612 1525
Fragments arranged by size:
1612 1525 824 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2015 2914 2015
Size: 899 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2711 3558 4714 1790
Size: 921 847 1156 2573
Fragments arranged by size:
2573 1156 921 847
BmgBI CAC'GTC
Cuts at: 1318 2368 1318
Size: 1050 4447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 2584 3228
Size: 416 53 83 399 24 975 192 644
Cuts at: 3228 4186 442
Size: 958 1753
Fragments arranged by size:
1753 975 958 644 416 399 192 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4157 1239
Size: 2918 2579
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 5497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2566 3622 4487 4785 2566
Size: 1056 865 298 3278
Fragments arranged by size:
3278 1056 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2466 3288 493
Size: 711 1262 822 2702
Fragments arranged by size:
2702 1262 822 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3304 3686 4321 275
Size: 53 83 186 936 1771 382 635 1451
Fragments arranged by size:
1771 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2645 3930 4328 4602 4749 5160 2645
Size: 1285 398 274 147 411 2982
Fragments arranged by size:
2982 1285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2038 2617 1186
Size: 844 8 579 4066
Fragments arranged by size:
4066 844 579 8
BseYI C'CCAG_C
Cuts at: 2481 4700 5196 2481
Size: 2219 496 2782
Fragments arranged by size:
2782 2219 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 1163
Size: 573 892 4032
Fragments arranged by size:
4032 892 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3558 3643 4714 3558
Size: 85 1071 4341
Fragments arranged by size:
4341 1071 85
BsmI GAATG_Cn'
Cuts at: 2064 2125 3008 3101 2064
Size: 61 883 93 4460
Fragments arranged by size:
4460 883 93 61
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 2320
Size: 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2298 2582 2711 2816 3558 3643
Size: 399 345 163 284 129 105 742 85
Cuts at: 3643 4714 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 345 284 163 129
105 85
BspEI T'CCGG_A
Cuts at: 2645 5160 2645
Size: 2515 2982
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2137 2293 2521 2600 2609 2743 2852 844
Size: 1293 156 228 79 9 134 109 3489
Fragments arranged by size:
3489 1293 228 156 134 109 79 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2239 2512 2728 3992 4166 65
Size: 1183 991 273 216 1264 174 1396
Fragments arranged by size:
1396 1264 1183 991 273 216 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 2477 2565 4141 4328 1700
Size: 110 270 397 88 1576 187 2869
Fragments arranged by size:
2869 1576 397 270 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 2728 1925
Size: 803 4694
BssSI C'ACGA_G
Cuts at: 2629 3557 4569 2629
Size: 928 1012 3557
Fragments arranged by size:
3557 1012 928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492
Size: 855 367 385 17 1452 86 12 346
Cuts at: 5492 1972
Size: 1977
Fragments arranged by size:
1977 1452 855 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2745 2745
Size: 5497
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4945
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2217 2227 3013 3807 3827 2130
Size: 87 10 786 794 20 3800
Fragments arranged by size:
3800 794 786 87 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 2944 3833
Size: 54 1425 50 275 18 415 699 889
Cuts at: 3833 5462 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 699 415 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2399 2475 3560 4157 4944 5212
Size: 393 445 322 76 1085 597 787 268
Cuts at: 5212 1239
Size: 1524
Fragments arranged by size:
1524 1085 787 597 445 393 322 268
76
EcoO109I rG'GnC_Cy
Cuts at: 2651 2651
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 4644 1678
Size: 2966 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 4138 4170 4212
Size: 32 135 32 695 32 1839 32 42
Cuts at: 4212 4244 1373
Size: 32 2626
Fragments arranged by size:
2626 1839 695 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2110 2841 3087 669
Size: 988 281 172 731 246 3079
Fragments arranged by size:
3079 988 731 281 246 172
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 2681 3455 3814 3973 518
Size: 648 1066 449 774 359 159 2042
Fragments arranged by size:
2042 1066 774 648 449 359 159
MspA1I CmG'CkG
Cuts at: 1606 2169 2913 3590 4738 4983 1606
Size: 563 744 677 1148 245 2120
Fragments arranged by size:
2120 1148 744 677 563 245
NaeI GCC'GGC
Cuts at: 2567 2567
Size: 5497
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4945
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NgoMIV G'CCGG_C
Cuts at: 2565 2565
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2481 1108
Size: 1373 4124
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PmlI CAC'GTG
Cuts at: 2466 2466
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PspXI vC'TCGA_Gb
Cuts at: 2748 2748
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2160 2529 2849 830
Size: 1330 369 320 3478
Fragments arranged by size:
3478 1330 369 320
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2160 2849 2160
Size: 689 4808
ScaI AGT'ACT
Cuts at: 2573 3745 5457 2573
Size: 1172 1712 2613
Fragments arranged by size:
2613 1712 1172
SexAI A'CCwGG_T
Cuts at: 2778 2778
Size: 5497
SfcI C'TryA_G
Cuts at: 826 1045 2156 2525 2845 3980 4661 4852 826
Size: 219 1111 369 320 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 369 320 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2581 2748 3601 4502 4764 820
Size: 197 926 638 167 853 901 262 1553
Fragments arranged by size:
1553 926 901 853 638 262 197 167
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 2460 1517
Size: 943 4554
StyI C'CwwG_G
Cuts at: 513 1065 2936 513
Size: 552 1871 3074
Fragments arranged by size:
3074 1871 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2200 2292 2671 3524 3541 3694 3879
Size: 1255 620 92 379 853 17 153 185
Cuts at: 3879 4324 325
Size: 445 1498
Fragments arranged by size:
1498 1255 853 620 445 379 185 153
92 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2571 3743
Size: 274 80 33 51 1443 423 171 1172
Cuts at: 3743 5455 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1172 423 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2183
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 2748 1943
Size: 805 4692
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI