Restriction map for
pFN21AE6256
(Circular) (Six-base) MAPSORT of: pFN21AE6256.seq Check: 7638 from: 1 to: 5332
pFN21AE6256 5332 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2100 2100
Size: 5332
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 1937
Size: 738 4594
Acc65I G'GTAC_C
Cuts at: 2653 2653
Size: 5332
AclI AA'CG_TT
Cuts at: 1563 3459 3832 1563
Size: 1896 373 3063
Fragments arranged by size:
3063 1896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 3395 4779 5047 1632
Size: 419 1344 1384 268 1917
Fragments arranged by size:
1917 1384 1344 419 268
AfeI AGC'GCT
Cuts at: 2496 2496
Size: 5332
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 4231 5208 1203
Size: 3028 977 1327
Fragments arranged by size:
3028 1327 977
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 3075 4647 3075
Size: 1572 3760
ApaI G_GGCC'C
Cuts at: 2070 2070
Size: 5332
ApaLI G'TGCA_C
Cuts at: 3389 4545 3389
Size: 1156 4176
ApoI r'AATT_y
Cuts at: 1493 2641 2867 3019 5128 1493
Size: 1148 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2657 1870
Size: 73 132 582 4545
Fragments arranged by size:
4545 582 132 73
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2653 4108 618
Size: 325 217 627 866 1455 1842
Fragments arranged by size:
1842 1455 866 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2651 1391
Size: 679 581 4072
Fragments arranged by size:
4072 679 581
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2314 928
Size: 971 415 3946
Fragments arranged by size:
3946 971 415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 1903
Size: 34 1586 34 3678
Fragments arranged by size:
3678 1586 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3224 4434 3224
Size: 1210 4122
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2279 2393 3943
Size: 107 122 71 631 605 607 114 1550
Cuts at: 3943 136
Size: 1525
Fragments arranged by size:
1550 1525 631 607 605 122 114 107
71
BglII A'GATC_T
Cuts at: 2442 5327 2442
Size: 2885 2447
BlpI GC'TnA_GC
Cuts at: 2613 2749 2613
Size: 136 5196
Bme1580I G_kGCm'C
Cuts at: 1790 2070 3393 4549 1790
Size: 280 1323 1156 2573
Fragments arranged by size:
2573 1323 1156 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 3063 4021
Size: 416 53 83 399 24 700 946 958
Cuts at: 4021 442
Size: 1753
Fragments arranged by size:
1753 958 946 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2312 2555 3992 1239
Size: 1007 66 243 1437 2579
Fragments arranged by size:
2579 1437 1007 243 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 3457 4322 4620 2396
Size: 1061 865 298 3108
Fragments arranged by size:
3108 1061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 3995 882
Size: 617 555 1941 2219
Fragments arranged by size:
2219 1941 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3123 493
Size: 711 1919 2702
Fragments arranged by size:
2702 1919 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3139 3521 4156 275
Size: 53 83 186 936 1606 382 635 1451
Fragments arranged by size:
1606 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2205 3765 4163 4437 4584 4995 2205
Size: 1560 398 274 147 411 2542
Fragments arranged by size:
2542 1560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 1178
Size: 30 165 30 1110 30 3967
Fragments arranged by size:
3967 1110 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2537 2540 1186
Size: 1351 3 3978
Fragments arranged by size:
3978 1351 3
BseYI C'CCAG_C
Cuts at: 2194 4535 5031 2194
Size: 2341 496 2495
Fragments arranged by size:
2495 2341 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 1163
Size: 573 720 4039
Fragments arranged by size:
4039 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2782 3543 4571 1540
Size: 275 186 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3393 3478 4549 3393
Size: 85 1071 4176
Fragments arranged by size:
4176 1071 85
BsmI GAATG_Cn'
Cuts at: 2843 2936 2843
Size: 93 5239
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2651 3393 3478 4549 1391
Size: 399 280 581 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 399 280 85
BspEI T'CCGG_A
Cuts at: 2205 4995 2205
Size: 2790 2542
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 2687 844
Size: 1256 587 3489
Fragments arranged by size:
3489 1256 587
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3219 1394
Size: 429 527 869 3507
Fragments arranged by size:
3507 869 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3976 4163 1700
Size: 110 2166 187 2869
Fragments arranged by size:
2869 2166 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 96
Size: 2499 2833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5332
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstYI r'GATC_y
Cuts at: 1972 2442 2541 2662 3029 3414 3431 4883 4969
Size: 470 99 121 367 385 17 1452 86
Cuts at: 4969 4981 5327 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 470 385 367 346 121 99
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2422 513
Size: 552 939 39 379 3423
Fragments arranged by size:
3423 939 552 379 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4593
Fragments arranged by size:
4593 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 3642 3662 2848
Size: 794 20 4518
Fragments arranged by size:
4518 794 20
ClaI AT'CG_AT
Cuts at: 1554 2577 1554
Size: 1023 4309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2636 2636
Size: 5332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4339 809
Size: 507 3023 1802
Fragments arranged by size:
3023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2779 3668
Size: 54 1425 50 275 18 186 763 889
Cuts at: 3668 5297 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 763 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2779 1537
Size: 275 967 4090
Fragments arranged by size:
4090 967 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2555 3262 1353
Size: 1202 707 3423
Fragments arranged by size:
3423 1202 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2534 3919 4293 4439 387
Size: 1064 138 945 1385 374 146 1280
Fragments arranged by size:
1385 1280 1064 945 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2246 2312 2555 3395 3992 4779
Size: 393 419 195 66 243 840 597 787
Cuts at: 4779 5047 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 597 419 393 268 243
195 66
EcoO109I rG'GnC_Cy
Cuts at: 2067 2118 2067
Size: 51 5281
EcoRI G'AATT_C
Cuts at: 2641 3019 2641
Size: 378 4954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 5332
FspI TGC'GCA
Cuts at: 3838 3838
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 2012 2285 2498 4479 1678
Size: 334 273 213 1981 2531
Fragments arranged by size:
2531 1981 334 273 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 3973 4005 4047
Size: 32 135 32 497 32 1872 32 42
Cuts at: 4047 4079 1373
Size: 32 2626
Fragments arranged by size:
2626 1872 497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 2560 2676 2922 669
Size: 988 281 445 177 116 246 3079
Fragments arranged by size:
3079 988 445 281 246 177 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5332
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KpnI G_GTAC'C
Cuts at: 2657 2657
Size: 5332
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4446 4630 1305
Size: 3141 184 2007
Fragments arranged by size:
3141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3510
Fragments arranged by size:
3510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 3290 3649 3808 518
Size: 648 1437 687 359 159 2042
Fragments arranged by size:
2042 1437 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2045 2748 3425 4573 4818 1606
Size: 432 7 703 677 1148 245 2120
Fragments arranged by size:
2120 1148 703 677 432 245 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 2422 513
Size: 552 939 418 3423
Fragments arranged by size:
3423 939 552 418
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5332
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2690 4235 1132
Size: 97 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5332
PciI A'CATG_T
Cuts at: 4231 4231
Size: 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5332
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5332
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 4914 4946 3401
Size: 32 1481 32 3787
Fragments arranged by size:
3787 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2118
Size: 5332
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5332
PsiI TTA'TAA
Cuts at: 2902 2902
Size: 5332
PspOMI G'GGCC_C
Cuts at: 2066 2066
Size: 5332
PspXI vC'TCGA_Gb
Cuts at: 2075 2075
Size: 5332
PstI C_TGCA'G
Cuts at: 830 2217 2684 830
Size: 1387 467 3478
Fragments arranged by size:
3478 1387 467
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 5332
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SanDI GG'GwC_CC
Cuts at: 2118 2118
Size: 5332
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 3580 5292 3580
Size: 1712 3620
SfcI C'TryA_G
Cuts at: 826 1045 2213 2680 3815 4496 4687 826
Size: 219 1168 467 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 681 467 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5332
SmaI CCC'GGG
Cuts at: 1872 2659 1872
Size: 787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2411 3436 4337 4599 820
Size: 197 926 132 336 1025 901 262 1553
Fragments arranged by size:
1553 1025 926 901 336 262 197 132
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 2304 2396 1517
Size: 787 92 4453
Fragments arranged by size:
4453 787 92
StyI C'CwwG_G
Cuts at: 513 1065 2004 2113 2391 2422 2502 2771 513
Size: 552 939 109 278 31 80 269 3074
Fragments arranged by size:
3074 939 552 278 269 109 80 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 3359 3376 3529 3714 4159 325
Size: 1255 1035 744 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1035 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3578 5290
Size: 274 80 33 51 1443 618 983 1712
Cuts at: 5290 96
Size: 138
Fragments arranged by size:
1712 1443 983 618 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 5292 5324 2513
Size: 32 2747 32 2521
Fragments arranged by size:
2747 2521 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5332
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 2075 1943
Size: 132 5200
XmaI C'CCGG_G
Cuts at: 1870 2657 1870
Size: 787 4545
XmnI GAAnn'nnTTC
Cuts at: 2444 3461 2444
Size: 1017 4315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PsrI PvuII RsrII SacI
SapI SexAI SfiI SfoI SrfI SwaI