Restriction map for
pFN21AE6251
(Circular) (Six-base) MAPSORT of: pFN21AE6251.seq Check: 9579 from: 1 to: 5497
pFN21AE6251 5497 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4944 5212 1632
Size: 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 2395 4396 5373 1203
Size: 1192 2001 977 1327
Fragments arranged by size:
2001 1327 1192 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 2157 1166
Size: 991 4506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2221 1184
Size: 1037 4460
AlwNI CAG_nnn'CTG
Cuts at: 3240 4812 3240
Size: 1572 3925
ApaI G_GGCC'C
Cuts at: 2654 2654
Size: 5497
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2306 2806 3032 3184 5293 1493
Size: 813 500 226 152 2109 1697
Fragments arranged by size:
2109 1697 813 500 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 1870
Size: 73 879 4545
Fragments arranged by size:
4545 879 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 4273 618
Size: 325 217 627 1031 1455 1842
Fragments arranged by size:
1842 1455 1031 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2654 2816 1391
Size: 1263 162 4072
Fragments arranged by size:
4072 1263 162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 928
Size: 971 766 3760
Fragments arranged by size:
3760 971 766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2526 2560 3688 3722 1903
Size: 34 589 34 1128 34 3678
Fragments arranged by size:
3678 1128 589 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2435 5492 2435
Size: 3057 2440
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2654 3558 4714 1790
Size: 864 904 1156 2573
Fragments arranged by size:
2573 1156 904 864
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2712 4157 1239
Size: 1076 397 1445 2579
Fragments arranged by size:
2579 1445 1076 397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 3622 4487 4785 2305
Size: 1317 865 298 3017
Fragments arranged by size:
3017 1317 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3304 3686 4321 275
Size: 53 83 186 936 1771 382 635 1451
Fragments arranged by size:
1771 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5497
BseYI C'CCAG_C
Cuts at: 2463 4700 5196 2463
Size: 2237 496 2764
Fragments arranged by size:
2764 2237 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2622 1163
Size: 573 886 4038
Fragments arranged by size:
4038 886 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3558 3643 4714 3558
Size: 85 1071 4341
Fragments arranged by size:
4341 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2654 2816 3558 3643 4714 1391
Size: 399 864 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 864 742 399 162 85
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 2698 3386 5116 2698
Size: 688 1730 3079
Fragments arranged by size:
3079 1730 688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 844
Size: 2008 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4141 4328 1700
Size: 110 2331 187 2869
Fragments arranged by size:
2869 2331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2609 794
Size: 1815 3682
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2593
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2335 2417 2435 2827 3194 3579 3596 5048
Size: 363 82 18 392 367 385 17 1452
Cuts at: 5048 5134 5146 5492 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 363 346 86
82 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4945
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 3013 3807 3827 2203
Size: 810 794 20 3873
Fragments arranged by size:
3873 810 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462
Size: 54 1425 50 275 18 1114 889 1629
Cuts at: 5462 8
Size: 43
Fragments arranged by size:
1629 1425 1114 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2160 4084 4458 4604 387
Size: 1064 138 571 1924 374 146 1280
Fragments arranged by size:
1924 1280 1064 571 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2712 3560 4157 4944 5212 1239
Size: 393 683 397 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 683 597 397 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2650 2651 2650
Size: 1 5496
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
EcoRV GAT'ATC
Cuts at: 2353 2353
Size: 5497
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 4644 1678
Size: 2966 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 4138 4170 4212
Size: 32 135 32 755 32 1779 32 42
Cuts at: 4212 4244 1373
Size: 32 2626
Fragments arranged by size:
2626 1779 755 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2742 2841 3087 669
Size: 988 281 804 99 246 3079
Fragments arranged by size:
3079 988 804 281 246 99
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 3879
HpaI GTT'AAC
Cuts at: 2742 3087 2742
Size: 345 5152
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2703 2793 3455 3814 3973 518
Size: 648 991 546 90 662 359 159 2042
Fragments arranged by size:
2042 991 662 648 546 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2913 3590 4738 4983 1606
Size: 1307 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4945
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2399 2855 4400 1132
Size: 97 1170 456 1545 2229
Fragments arranged by size:
2229 1545 1170 456 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 2395 4396 2395
Size: 2001 3496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PspOMI G'GGCC_C
Cuts at: 2650 2650
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2682 2845 3980 4661 4852 826
Size: 219 1637 163 1135 681 191 1471
Fragments arranged by size:
1637 1471 1135 681 219 191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2320 3601 4502 4764 820
Size: 197 926 377 1281 901 262 1553
Fragments arranged by size:
1553 1281 926 901 377 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 1517
Size: 762 4735
StyI C'CwwG_G
Cuts at: 513 1065 2936 513
Size: 552 1871 3074
Fragments arranged by size:
3074 1871 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2176 3524 3541 3694 3879 4324 325
Size: 1255 596 1348 17 153 185 445 1498
Fragments arranged by size:
1498 1348 1255 596 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2402 2434 5457 5489 2402
Size: 32 3023 32 2410
Fragments arranged by size:
3023 2410 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2594 1316
Size: 1278 4219
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2122 2290 2122
Size: 168 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI