Restriction map for
pFN21AE6241
(Circular) (Six-base) MAPSORT of: pFN21AE6241.seq Check: 73 from: 1 to: 5533
pFN21AE6241 5533 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2757 3596 4980 5248 1632
Size: 1125 839 1384 268 1917
Fragments arranged by size:
1917 1384 1125 839 268
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 5533
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 2297 1166
Size: 1131 4402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 3276 4848 3276
Size: 1572 3961
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2595 2729 2842 3068 3220 5329 1493
Size: 1102 134 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1102 226 152 134 113
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2858 1870
Size: 73 915 4545
Fragments arranged by size:
4545 915 73
BamHI G'GATC_C
Cuts at: 2061 2863 3230 2061
Size: 802 367 4364
Fragments arranged by size:
4364 802 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2431 2530 2854 4309
Size: 325 217 627 351 293 99 324 1455
Cuts at: 4309 618
Size: 1842
Fragments arranged by size:
1842 1455 627 351 325 324 293 217
99
BanII G_rGCy'C
Cuts at: 1391 2016 2194 2234 2852 1391
Size: 625 178 40 618 4072
Fragments arranged by size:
4072 625 618 178 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4562
BbvCI CC'TCA_GC
Cuts at: 2508 2508
Size: 5533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2218 2221 4144
Size: 107 122 71 631 605 546 3 1923
Cuts at: 4144 136
Size: 1525
Fragments arranged by size:
1923 1525 631 605 546 122 107 71
3
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2950 2950
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1790 2808 3594 4750 1790
Size: 1018 786 1156 2573
Fragments arranged by size:
2573 1156 1018 786
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 4193 1239
Size: 1235 1719 2579
Fragments arranged by size:
2579 1719 1235
Bpu10I CC'TnA_GC
Cuts at: 2508 2508
Size: 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 4196 882
Size: 617 580 2117 2219
Fragments arranged by size:
2219 2117 617 580
BsaAI yAC'GTr
Cuts at: 493 1204 2299 3324 493
Size: 711 1095 1025 2702
Fragments arranged by size:
2702 1095 1025 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2330 3340 3722
Size: 53 83 186 936 487 310 1010 382
Cuts at: 3722 4357 275
Size: 635 1451
Fragments arranged by size:
1451 1010 936 635 487 382 310 186
83 53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2276 1186
Size: 1087 3 4443
Fragments arranged by size:
4443 1087 3
BseYI C'CCAG_C
Cuts at: 2323 4736 5232 2323
Size: 2413 496 2624
Fragments arranged by size:
2624 2413 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2529 1163
Size: 573 793 4167
Fragments arranged by size:
4167 793 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2234 3594 3679 4750 2234
Size: 1360 85 1071 3017
Fragments arranged by size:
3017 1360 1071 85
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2194 2234 2808 2852 3594 3679
Size: 399 226 178 40 574 44 742 85
Cuts at: 3679 4750 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 574 399 226 178 85
44 40
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2390 2888 844
Size: 1546 498 3489
Fragments arranged by size:
3489 1546 498
BsrBI CCG'CTC
Cuts at: 1394 1823 2266 3420 1394
Size: 429 443 1154 3507
Fragments arranged by size:
3507 1154 443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2204 4028 4202 65
Size: 1183 956 1824 174 1396
Fragments arranged by size:
1824 1396 1183 956 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 2250 1925
Size: 325 5208
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 2812 794
Size: 1611 407 3515
Fragments arranged by size:
3515 1611 407
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstXI CCAn_nnnn'nTGG
Cuts at: 2375 2375
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2061 2740 2863 3230 3615 3632 5084 5170
Size: 89 679 123 367 385 17 1452 86
Cuts at: 5170 5182 5528 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 679 385 367 346 123 89
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2216 2313 513
Size: 552 939 212 97 3733
Fragments arranged by size:
3733 939 552 212 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 3843 3863 3049
Size: 794 20 4719
Fragments arranged by size:
4719 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2980 3869
Size: 54 1425 50 275 18 471 679 889
Cuts at: 3869 5498 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 679 471 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3463 1353
Size: 2110 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2232 2232
Size: 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2757 3596 4193 4980 5248 1239
Size: 393 842 283 839 597 787 268 1524
Fragments arranged by size:
1524 842 839 787 597 393 283 268
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2780 2780
Size: 5533
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2059 2179 4680 1678
Size: 381 120 2501 2531
Fragments arranged by size:
2531 2501 381 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4174 4206 4248 4280 1373
Size: 32 135 32 2602 32 42 32 2626
Fragments arranged by size:
2626 2602 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 4647 4831 1305
Size: 797 2545 184 2007
Fragments arranged by size:
2545 2007 797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3711
Fragments arranged by size:
3711 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 3491 3850 4009 518
Size: 648 1131 1194 359 159 2042
Fragments arranged by size:
2042 1194 1131 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2218 2306 2949 3626 4774 5019 1606
Size: 612 88 643 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 643 612 245 88
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4042
Fragments arranged by size:
4042 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2323 2483 2891 4436 1132
Size: 97 1094 160 408 1545 2229
Fragments arranged by size:
2229 1545 1094 408 160 97
PasI CC'CwG_GG
Cuts at: 1348 2069 1348
Size: 721 4812
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2591 1108
Size: 1483 4050
PfoI T'CCnGG_A
Cuts at: 2023 2821 2023
Size: 798 4735
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2092 2183 2885 830
Size: 1262 91 702 3478
Fragments arranged by size:
3478 1262 702 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
SacI G_AGCT'C
Cuts at: 2234 2234
Size: 5533
SacII CC_GC'GG
Cuts at: 2219 2219
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2088 2179 2881 4016 4697 4888 826
Size: 219 1043 91 702 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 702 681 219 191 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2860 1872
Size: 988 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2323 2483 2891 1132
Size: 1191 160 408 3774
Fragments arranged by size:
3774 1191 408 160
SspI AAT'ATT
Cuts at: 5 52 2611 3457 5
Size: 47 2559 846 2081
Fragments arranged by size:
2559 2081 846 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2972 513
Size: 552 939 968 3074
Fragments arranged by size:
3074 968 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3560 3577 3730 3915 4360 325
Size: 1255 1980 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2122 2154 5493 5525 2122
Size: 32 3339 32 2130
Fragments arranged by size:
3339 2130 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2858 1870
Size: 988 4545
XmnI GAAnn'nnTTC
Cuts at: 2240 3662 2240
Size: 1422 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI