Restriction map for
pFN21AE6237
(Circular) (Six-base) MAPSORT of: pFN21AE6237.seq Check: 5020 from: 1 to: 5425
pFN21AE6237 5425 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2478 2746 2478
Size: 268 5157
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3488 4872 5140 1632
Size: 1856 1384 268 1917
Fragments arranged by size:
1917 1856 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 2115 4324 5301 1203
Size: 912 2209 977 1327
Fragments arranged by size:
2209 1327 977 912
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AlwNI CAG_nnn'CTG
Cuts at: 2472 3168 4740 2472
Size: 696 1572 3157
Fragments arranged by size:
3157 1572 696
ApaI G_GGCC'C
Cuts at: 2394 2513 2649 2394
Size: 119 136 5170
Fragments arranged by size:
5170 136 119
ApaLI G'TGCA_C
Cuts at: 2348 3482 4638 2348
Size: 1134 1156 3135
Fragments arranged by size:
3135 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2431 2648 2750 1870
Size: 73 289 199 217 102 4545
Fragments arranged by size:
4545 289 217 199 102 73
BamHI G'GATC_C
Cuts at: 2106 2755 3122 2106
Size: 649 367 4409
Fragments arranged by size:
4409 649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2478 2746 4201 618
Size: 325 217 627 258 433 268 1455 1842
Fragments arranged by size:
1842 1455 627 433 325 268 258 217
BanII G_rGCy'C
Cuts at: 1391 2085 2394 2513 2649 2655 2744 1391
Size: 694 309 119 136 6 89 4072
Fragments arranged by size:
4072 694 309 136 119 89 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3616 3650 1903
Size: 34 50 34 1595 34 3678
Fragments arranged by size:
3678 1595 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2492 2574
Size: 107 122 71 631 605 337 483 82
Cuts at: 2574 4036 136
Size: 1462 1525
Fragments arranged by size:
1525 1462 631 605 483 337 122 107
82 71
BglII A'GATC_T
Cuts at: 2136 5420 2136
Size: 3284 2141
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2352 2394 2513 2649 3486 4642 1790
Size: 562 42 119 136 837 1156 2573
Fragments arranged by size:
2573 1156 837 562 136 119 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2314 2321 2494 4085 1239
Size: 1075 7 173 1591 2579
Fragments arranged by size:
2579 1591 1075 173 7
Bpu10I CC'TnA_GC
Cuts at: 2366 2366
Size: 5425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4088 882
Size: 617 2589 2219
Fragments arranged by size:
2589 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 3216 493
Size: 711 1143 869 2702
Fragments arranged by size:
2702 1143 869 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2220 2244 2286
Size: 53 83 186 936 522 165 24 42
Cuts at: 2286 3232 3614 4249 275
Size: 946 382 635 1451
Fragments arranged by size:
1451 946 936 635 522 382 186 165
83 53 42 24
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5200
Fragments arranged by size:
5200 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5425
BseYI C'CCAG_C
Cuts at: 4628 5124 4628
Size: 496 4929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 1163
Size: 573 483 4369
Fragments arranged by size:
4369 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2316 2875 3636 4664 1540
Size: 275 186 315 559 761 1028 2301
Fragments arranged by size:
2301 1028 761 559 315 275 186
BsiHKAI G_wGCw'C
Cuts at: 2352 2679 3486 3571 4642 2352
Size: 327 807 85 1071 3135
Fragments arranged by size:
3135 1071 807 327 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2352 2394 2513 2649 2655 2679
Size: 399 295 267 42 119 136 6 24
Cuts at: 2679 2744 3486 3571 4642 1391
Size: 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 267 136 119
85 65 42 24 6
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 844
Size: 1936 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2240 2486 2610 4069 4256 1700
Size: 110 232 198 246 124 1459 187 2869
Fragments arranged by size:
2869 1459 246 232 198 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2045 794
Size: 1251 4174
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 2236 1184
Size: 1052 4373
BstYI r'GATC_y
Cuts at: 1972 2106 2136 2755 3122 3507 3524 4976 5062
Size: 134 30 619 367 385 17 1452 86
Cuts at: 5062 5074 5420 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 619 385 367 346 134 86
30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2535 2569 513
Size: 552 1470 34 3369
Fragments arranged by size:
3369 1470 552 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2409 2493
Size: 54 1425 50 275 18 378 201 84
Cuts at: 2493 2872 3761 5390 8
Size: 379 889 1629 43
Fragments arranged by size:
1629 1425 889 379 378 275 201 84
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 3355 1353
Size: 905 1097 3423
Fragments arranged by size:
3423 1097 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 2114 4012 4386 4532 387
Size: 1064 138 504 21 1898 374 146 1280
Fragments arranged by size:
1898 1280 1064 504 374 146 138 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2314 2321 2494 3488 4085 4872 5140
Size: 393 682 7 173 994 597 787 268
Cuts at: 5140 1239
Size: 1524
Fragments arranged by size:
1524 994 787 682 597 393 268 173
7
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2369
Size: 5425
EcoO109I rG'GnC_Cy
Cuts at: 2362 2391 2362
Size: 29 5396
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2015 2665 4572 1678
Size: 337 650 1907 2531
Fragments arranged by size:
2531 1907 650 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373
Size: 32 135 32 2494 32 42 32 2626
Fragments arranged by size:
2626 2494 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2482 2750 2482
Size: 268 5157
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3383 3742 3901 518
Size: 648 1179 1038 359 159 2042
Fragments arranged by size:
2042 1179 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2407 2571 2841 3518 4666 4911 1606
Size: 552 249 164 270 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 270 249 245 164
NaeI GCC'GGC
Cuts at: 2488 2612 2488
Size: 124 5301
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NgoMIV G'CCGG_C
Cuts at: 2486 2610 2486
Size: 124 5301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2119 2783 4328 1132
Size: 97 890 664 1545 2229
Fragments arranged by size:
2229 1545 890 664 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 2115 4324 2115
Size: 2209 3216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PmlI CAC'GTG
Cuts at: 2347 2347
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5425
PshAI GACnn'nnGTC
Cuts at: 1613 2243 1613
Size: 630 4795
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2390 2509 2645 2390
Size: 119 136 5170
Fragments arranged by size:
5170 136 119
PstI C_TGCA'G
Cuts at: 830 2777 830
Size: 1947 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
RsrII CG'GwC_CG
Cuts at: 2455 2455
Size: 5425
SacII CC_GC'GG
Cuts at: 2572 2572
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2773 3908 4589 4780 826
Size: 219 1728 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2234 2433 2650 2752 1872
Size: 362 199 217 102 4545
Fragments arranged by size:
4545 362 217 199 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SrfI GCCC'GGGC
Cuts at: 2650 2650
Size: 5425
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2124 2864 513
Size: 552 1059 740 3074
Fragments arranged by size:
3074 1059 740 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 3452 3469 3622 3807 4252 325
Size: 1255 750 1122 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1122 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2232 2431 2648 2750 1870
Size: 362 199 217 102 4545
Fragments arranged by size:
4545 362 217 199 102
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspXI PsrI PvuII SacI SanDI SapI
SexAI SfiI SfoI SwaI XcmI