Restriction map for
pFN21AE6236
(Circular) (Six-base) MAPSORT of: pFN21AE6236.seq Check: 7335 from: 1 to: 5422
pFN21AE6236 5422 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2638 2638
Size: 5422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3485 4869 5137 1632
Size: 1853 1384 268 1917
Fragments arranged by size:
1917 1853 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 2018 2132 1166
Size: 852 114 4456
Fragments arranged by size:
4456 852 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 4570
AlwNI CAG_nnn'CTG
Cuts at: 2029 2494 3165 4737 2029
Size: 465 671 1572 2714
Fragments arranged by size:
2714 1572 671 465
ApaI G_GGCC'C
Cuts at: 2671 2671
Size: 5422
ApaLI G'TGCA_C
Cuts at: 2314 3479 4635 2314
Size: 1165 1156 3101
Fragments arranged by size:
3101 1165 1156
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2275 2747 1870
Size: 73 332 472 4545
Fragments arranged by size:
4545 472 332 73
BamHI G'GATC_C
Cuts at: 2752 3119 2752
Size: 367 5055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2369 2537 2569 2743
Size: 325 217 627 327 255 168 32 174
Cuts at: 2743 4198 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 327 325 255 217 174
168 32
BanII G_rGCy'C
Cuts at: 1391 2154 2671 2741 1391
Size: 763 517 70 4072
Fragments arranged by size:
4072 763 517 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 2070 1548
Size: 522 4900
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2092 4033
Size: 107 122 71 631 605 343 77 1941
Cuts at: 4033 136
Size: 1525
Fragments arranged by size:
1941 1525 631 605 343 122 107 77
71
BglII A'GATC_T
Cuts at: 2199 5417 2199
Size: 3218 2204
BlpI GC'TnA_GC
Cuts at: 2042 2839 2042
Size: 797 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2117 2318 2336 2671 3483 4639 1790
Size: 276 51 201 18 335 812 1156 2573
Fragments arranged by size:
2573 1156 812 335 276 201 51 18
BmgBI CAC'GTC
Cuts at: 1318 2431 1318
Size: 1113 4309
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4111 442
Size: 416 53 83 399 24 1736 958 1753
Fragments arranged by size:
1753 1736 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 4082 1239
Size: 980 1863 2579
Fragments arranged by size:
2579 1863 980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 2719 3213 493
Size: 711 906 609 494 2702
Fragments arranged by size:
2702 906 711 609 494
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3229 3611 4246 275
Size: 53 83 186 936 1696 382 635 1451
Fragments arranged by size:
1696 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 1178
Size: 30 165 30 750 30 4417
Fragments arranged by size:
4417 750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2503 1186
Size: 1317 4105
BseYI C'CCAG_C
Cuts at: 2462 4625 5121 2462
Size: 2163 496 2763
Fragments arranged by size:
2763 2163 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2456 1163
Size: 573 306 414 4129
Fragments arranged by size:
4129 573 414 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2133 2872 3633 4661 1540
Size: 275 186 18 114 739 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 275 186 114 18
BsiHKAI G_wGCw'C
Cuts at: 2318 3483 3568 4639 2318
Size: 1165 85 1071 3101
Fragments arranged by size:
3101 1165 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
BsmBI CGTCTCn'nnnn_
Cuts at: 2416 2416
Size: 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2117 2154 2318 2336 2671 2741
Size: 399 276 51 37 164 18 335 70
Cuts at: 2741 3483 3568 4639 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 276 164 85
70 51 37 18
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2638 2777 844
Size: 1794 139 3489
Fragments arranged by size:
3489 1794 139
BsrBI CCG'CTC
Cuts at: 1394 1823 3309 1394
Size: 429 1486 3507
Fragments arranged by size:
3507 1486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2387 4066 4253 1700
Size: 110 577 1679 187 2869
Fragments arranged by size:
2869 1679 577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2286 2322 794
Size: 1492 36 3894
Fragments arranged by size:
3894 1492 36
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2431
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2199 2752 3119 3504 3521 4973 5059 5071
Size: 227 553 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 553 385 367 346 227 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2613 2938 3732 3752 2613
Size: 325 794 20 4283
Fragments arranged by size:
4283 794 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2016 2130
Size: 54 1425 50 275 18 177 9 114
Cuts at: 2130 2385 2406 2485 2685 2869 3758 5387 8
Size: 255 21 79 200 184 889 1629 43
Fragments arranged by size:
1629 1425 889 275 255 200 184 177
114 79 54 50 43 21 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2130 2869 1537
Size: 275 204 114 739 4090
Fragments arranged by size:
4090 739 275 204 114
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 3352 1353
Size: 1028 971 3423
Fragments arranged by size:
3423 1028 971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 4009 4383 4529 387
Size: 1064 138 521 1899 374 146 1280
Fragments arranged by size:
1899 1280 1064 521 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 3485 4082 4869 5137 1239
Size: 393 587 1266 597 787 268 1524
Fragments arranged by size:
1524 1266 787 597 587 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2491 2580 2590 2668 2491
Size: 89 10 78 5245
Fragments arranged by size:
5245 89 78 10
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5422
FseI GG_CCGG'CC
Cuts at: 2391 2391
Size: 5422
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 2222 4569 1678
Size: 544 2347 2531
Fragments arranged by size:
2531 2347 544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2487 10
Size: 54 1425 343 177 478 2945
Fragments arranged by size:
2945 1425 478 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2132 2306 3380 3739 3898 518
Size: 648 852 114 174 1074 359 159 2042
Fragments arranged by size:
2042 1074 852 648 359 174 159 114
MspA1I CmG'CkG
Cuts at: 1606 2455 2466 2648 2697 2838 3515 4663 4908
Size: 849 11 182 49 141 677 1148 245
Cuts at: 4908 1606
Size: 2120
Fragments arranged by size:
2120 1148 849 677 245 182 141 49
11
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 5422
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2598 1348
Size: 1250 4172
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2414 2414
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PmlI CAC'GTG
Cuts at: 2110 2110
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2667 2667
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2774 830
Size: 1944 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2648 2648
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2575 2770 3905 4586 4777 826
Size: 219 1530 195 1135 681 191 1471
Fragments arranged by size:
1530 1471 1135 681 219 195 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2015 2015
Size: 5422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2328 2861 513
Size: 552 939 324 533 3074
Fragments arranged by size:
3074 939 552 533 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2149 2153 2181 2185 5382 5414 2149
Size: 4 28 4 3197 32 2157
Fragments arranged by size:
3197 2157 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2428
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FspAI KasI NarI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI