Restriction map for
pFN21AE6234
(Circular) (Six-base) MAPSORT of: pFN21AE6234.seq Check: 6629 from: 1 to: 5590
pFN21AE6234 5590 bp
With 182 enzymes: SgfI *
July 20, 2018 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2390 2390
Size: 5590
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2208 2911 2208
Size: 703 4887
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 2457
Size: 32 5558
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2343 2920 3287 2343
Size: 577 367 4646
Fragments arranged by size:
4646 577 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 2275 2614 2911 4366
Size: 325 217 627 421 67 339 297 1455
Cuts at: 4366 618
Size: 1842
Fragments arranged by size:
1842 1455 627 421 339 325 297 217
67
BanII G_rGCy'C
Cuts at: 1391 2134 2462 2909 1391
Size: 743 328 447 4072
Fragments arranged by size:
4072 743 447 328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 2518 2710 2879 928
Size: 971 171 448 192 169 3639
Fragments arranged by size:
3639 971 448 192 171 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1998 2032 3781 3815 1903
Size: 34 61 34 1749 34 3678
Fragments arranged by size:
3678 1749 61 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 4462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4201 136
Size: 107 122 71 631 605 2529 1525
Fragments arranged by size:
2529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2619 3651 4807 1790
Size: 829 1032 1156 2573
Fragments arranged by size:
2573 1156 1032 829
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 3321 4279
Size: 416 53 83 399 24 1044 860 958
Cuts at: 4279 442
Size: 1753
Fragments arranged by size:
1753 1044 958 860 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 2043
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2873 4250 1239
Size: 1245 389 1377 2579
Fragments arranged by size:
2579 1377 1245 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 2262 4253 882
Size: 617 515 248 1991 2219
Fragments arranged by size:
2219 1991 617 515 248
BsaAI yAC'GTr
Cuts at: 493 1204 2735 3381 493
Size: 711 1531 646 2702
Fragments arranged by size:
2702 1531 711 646
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2346 2586 4023 4421 4695 4842 5253 2346
Size: 240 1437 398 274 147 411 2683
Fragments arranged by size:
2683 1437 411 398 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2457 2487 1178
Size: 30 165 30 786 30 238 30 4281
Fragments arranged by size:
4281 786 238 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2048 2051 2054 2057 2426 2468 2489
Size: 859 3 3 3 3 369 42 21
Cuts at: 2489 2861 2868 1186
Size: 372 7 3908
Fragments arranged by size:
3908 859 372 369 42 21 7 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2033 4793 5289 2033
Size: 2760 496 2334
Fragments arranged by size:
2760 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5017
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 3651 3736 4807 2499
Size: 1152 85 1071 3282
Fragments arranged by size:
3282 1152 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2462 2499 2619 2909 3651 3736
Size: 399 344 328 37 120 290 742 85
Cuts at: 3736 4807 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 328 290 120
85 37
BspEI T'CCGG_A
Cuts at: 2346 5253 2346
Size: 2907 2683
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2390 2945 844
Size: 1546 555 3489
Fragments arranged by size:
3489 1546 555
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 4085 4259 65
Size: 1183 1179 1658 174 1396
Fragments arranged by size:
1658 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2650 4234 4421 1700
Size: 110 840 1584 187 2869
Fragments arranged by size:
2869 1584 840 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2343 2440 2920 3287 3672 3689 5141 5227
Size: 371 97 480 367 385 17 1452 86
Cuts at: 5227 5239 5585 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 480 385 371 367 346 97
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3900 3920 3106
Size: 794 20 4776
Fragments arranged by size:
4776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555
Size: 54 1425 50 275 18 1207 889 1629
Cuts at: 5555 8
Size: 43
Fragments arranged by size:
1629 1425 1207 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 2749 2800 3520 1353
Size: 1368 28 51 720 3423
Fragments arranged by size:
3423 1368 720 51 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2873 3653 4250 5037 5305 1239
Size: 393 852 389 780 597 787 268 1524
Fragments arranged by size:
1524 852 787 780 597 393 389 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2224 2224
Size: 5590
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 4737 1678
Size: 3059 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 2563 2581 2595
Size: 32 135 32 537 32 422 18 14
Cuts at: 2595 2613 4231 4263 4305 4337 1373
Size: 18 1618 32 42 32 2626
Fragments arranged by size:
2626 1618 537 422 135 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2011 748
Size: 1263 4327
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2212 2915 2212
Size: 703 4887
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3768
Fragments arranged by size:
3768 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 3548 3907 4066 518
Size: 648 1032 1350 359 159 2042
Fragments arranged by size:
2042 1350 1032 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 3006 3683 4831 5076 1606
Size: 431 969 677 1148 245 2120
Fragments arranged by size:
2120 1148 969 677 431 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2948 4493 1132
Size: 97 1719 1545 2229
Fragments arranged by size:
2229 1719 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2457 2489 3659 3691 5172 5204 2457
Size: 32 1170 32 1481 32 2843
Fragments arranged by size:
2843 1481 1170 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 2757 3160 2757
Size: 403 5187
PstI C_TGCA'G
Cuts at: 830 2508 2541 2942 830
Size: 1678 33 401 3478
Fragments arranged by size:
3478 1678 401 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2037 2037
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SapI GCTCTTCn'nnn_
Cuts at: 2721 2721
Size: 5590
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2187 2242 2504 2537 2938 4073 4754
Size: 219 1142 55 262 33 401 1135 681
Cuts at: 4754 4945 826
Size: 191 1471
Fragments arranged by size:
1471 1142 1135 681 401 262 219 191
55 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3694 4595 4857 820
Size: 197 926 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2735 3381 493
Size: 2242 646 2702
Fragments arranged by size:
2702 2242 646
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2664 3029 513
Size: 552 939 660 365 3074
Fragments arranged by size:
3074 939 660 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 2852 3617 3634 3787 3972 4417
Size: 1255 863 409 765 17 153 185 445
Cuts at: 4417 325
Size: 1498
Fragments arranged by size:
1498 1255 863 765 445 409 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 3836 5548
Size: 274 80 33 51 1443 564 1295 1712
Cuts at: 5548 96
Size: 138
Fragments arranged by size:
1712 1443 1295 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 5550 5582 2189
Size: 32 3329 32 2197
Fragments arranged by size:
3329 2197 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI